Protein Unfolding: Denaturant vs. Force
Round 1
Reviewer 1 Report
Protein folding and unfolding biology is very challenging, the current ms work compares the energetic barriers and folding rates derived from chemical, thermal, and mechanical experiments using an immunoglobulin-like domain from the muscle protein as a model system. Author drawn various method to understand protein unfolding process. The paper is clear and presented data clearly with minimal experiments. I don’t have any further comments, and this is outstanding paper in understanding molecular and biophysical analysis of protein function in general.
Author Response
Thank you for the kind comments.
Reviewer 2 Report
Manuscript submitted by Kelly et. al "Protein Unfolding: Denaturant vs Force" in Biomedicines (biomedicines-1392288). The presented data will be important in terms of protein unfolding. In this regard, this paper is worth for publication in the journal. However some changes and update is necessary. Comments:
- Page 5, section 3.1: line “Protein refolding experiments……………..of mechanical force”. Author should cite these articles to support the sentence with literature: “Cell Biochem Biophys 2012, 62, 487-499”. ‘Cell Biochem Biophys. 2011; 61, 551-560”.
- Page 5, section 3.1: line “This suggests that, while the……………requirement of refolding following thermal denaturation.” To support this sentence with literature author should cite article: “RSC Advances 2015, 5 (26), 20115-20131”. “Appl Microbiol Biotechnol 2014, 98, 2533-2543”.
Author Response
Thank you for the suggested references. We have added those to the manuscript.
