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Article

Novel Bradykinin-Potentiating Peptides and Three-Finger Toxins from Viper Venom: Combined NGS Venom Gland Transcriptomics and Quantitative Venom Proteomics of the Azemiops feae Viper

1
Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia
2
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997 Moscow, Russia
3
Institute of Chemistry and Biochemistry, Freie Universität Berlin, 14195 Berlin, Germany
4
Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, 142290 Moscow Region, Russia
5
Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City 700000, Vietnam
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Biomedicines 2020, 8(8), 249; https://doi.org/10.3390/biomedicines8080249
Received: 29 June 2020 / Revised: 24 July 2020 / Accepted: 24 July 2020 / Published: 28 July 2020
(This article belongs to the Special Issue Animal Venoms–Curse or Cure?)
Feae’s viper Azemipos feae belongs to the Azemiopinae subfamily of the Viperidae family. The effects of Viperidae venoms are mostly coagulopathic with limited neurotoxicity manifested by phospholipases A2. From A. feae venom, we have earlier isolated azemiopsin, a novel neurotoxin inhibiting the nicotinic acetylcholine receptor. To characterize other A. feae toxins, we applied label-free quantitative proteomics, which revealed 120 unique proteins, the most abundant being serine proteinases and phospholipases A2. In total, toxins representing 14 families were identified, among which bradykinin-potentiating peptides with unique amino acid sequences possessed biological activity in vivo. The proteomic analysis revealed also basal (commonly known as non-conventional) three-finger toxins belonging to the group of those possessing neurotoxic activity. This is the first indication of the presence of three-finger neurotoxins in viper venom. In parallel, the transcriptomic analysis of venom gland performed by Illumina next-generation sequencing further revealed 206 putative venom transcripts. Together, the study unveiled the venom proteome and venom gland transciptome of A. feae, which in general resemble those of other snakes from the Viperidae family. However, new toxins not found earlier in viper venom and including three-finger toxins and unusual bradykinin-potentiating peptides were discovered. View Full-Text
Keywords: Feae’s viper; Azemiops feae; venom; venom gland; proteomics; transcriptomic; bradykinin-potentiating peptides; three-finger toxins Feae’s viper; Azemiops feae; venom; venom gland; proteomics; transcriptomic; bradykinin-potentiating peptides; three-finger toxins
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MDPI and ACS Style

Babenko, V.V.; Ziganshin, R.H.; Weise, C.; Dyachenko, I.; Shaykhutdinova, E.; Murashev, A.N.; Zhmak, M.; Starkov, V.; Hoang, A.N.; Tsetlin, V.; Utkin, Y. Novel Bradykinin-Potentiating Peptides and Three-Finger Toxins from Viper Venom: Combined NGS Venom Gland Transcriptomics and Quantitative Venom Proteomics of the Azemiops feae Viper. Biomedicines 2020, 8, 249. https://doi.org/10.3390/biomedicines8080249

AMA Style

Babenko VV, Ziganshin RH, Weise C, Dyachenko I, Shaykhutdinova E, Murashev AN, Zhmak M, Starkov V, Hoang AN, Tsetlin V, Utkin Y. Novel Bradykinin-Potentiating Peptides and Three-Finger Toxins from Viper Venom: Combined NGS Venom Gland Transcriptomics and Quantitative Venom Proteomics of the Azemiops feae Viper. Biomedicines. 2020; 8(8):249. https://doi.org/10.3390/biomedicines8080249

Chicago/Turabian Style

Babenko, Vladislav V., Rustam H. Ziganshin, Christoph Weise, Igor Dyachenko, Elvira Shaykhutdinova, Arkady N. Murashev, Maxim Zhmak, Vladislav Starkov, Anh Ngoc Hoang, Victor Tsetlin, and Yuri Utkin. 2020. "Novel Bradykinin-Potentiating Peptides and Three-Finger Toxins from Viper Venom: Combined NGS Venom Gland Transcriptomics and Quantitative Venom Proteomics of the Azemiops feae Viper" Biomedicines 8, no. 8: 249. https://doi.org/10.3390/biomedicines8080249

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