Novel Bradykinin-Potentiating Peptides and Three-Finger Toxins from Viper Venom: Combined NGS Venom Gland Transcriptomics and Quantitative Venom Proteomics of the Azemiops feae Viper
Abstract
:1. Introduction
2. Experimental Section
2.1. Specimen Collection and Tissue Preparation
2.2. cDNA Library Preparation and Sequencing
2.2.1. cDNA Synthesis and cDNA Amplification
2.2.2. Illumina Sequencing
2.3. NGS Data Analysis
2.4. Other Computational Tool
2.5. BioProject and Raw Sequence Data
2.6. Reduction, Alkylation and Digestion of the Proteins
2.7. Tryptic Peptides Desalting
2.8. Liquid Chromatography and Mass Spectrometry
2.9. Data Analysis
2.10. Characterization of Bradykinin-Potentiating Peptides by MALDI Mass Spectrometry
2.11. Peptide Synthesis
2.12. Blood Pressure Measurements
3. Results
3.1. Research Outline: RNA Sequencing and Analysis of the Initial Data
3.2. Venom-Gland Transcriptome
3.3. Venom Proteomics/Peptidomics
3.3.1. Serine Proteases
3.3.2. Phospholipases A2
3.3.3. Cysteine-Rich Seceretory Proteins
3.3.4. L-Amino Acid Oxidase
3.3.5. Snake Venom Metalloproteinase
3.3.6. Snake Venom Vascular Endothelial Growth Factor
3.3.7. Three-Finger Toxins
3.3.8. Bradykinin-Potentiating Peptides and Azemiopsin
3.3.9. Biological Activity of New Bradykinin-Potentiating Peptides
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Babenko, V.V.; Ziganshin, R.H.; Weise, C.; Dyachenko, I.; Shaykhutdinova, E.; Murashev, A.N.; Zhmak, M.; Starkov, V.; Hoang, A.N.; Tsetlin, V.; et al. Novel Bradykinin-Potentiating Peptides and Three-Finger Toxins from Viper Venom: Combined NGS Venom Gland Transcriptomics and Quantitative Venom Proteomics of the Azemiops feae Viper. Biomedicines 2020, 8, 249. https://doi.org/10.3390/biomedicines8080249
Babenko VV, Ziganshin RH, Weise C, Dyachenko I, Shaykhutdinova E, Murashev AN, Zhmak M, Starkov V, Hoang AN, Tsetlin V, et al. Novel Bradykinin-Potentiating Peptides and Three-Finger Toxins from Viper Venom: Combined NGS Venom Gland Transcriptomics and Quantitative Venom Proteomics of the Azemiops feae Viper. Biomedicines. 2020; 8(8):249. https://doi.org/10.3390/biomedicines8080249
Chicago/Turabian StyleBabenko, Vladislav V., Rustam H. Ziganshin, Christoph Weise, Igor Dyachenko, Elvira Shaykhutdinova, Arkady N. Murashev, Maxim Zhmak, Vladislav Starkov, Anh Ngoc Hoang, Victor Tsetlin, and et al. 2020. "Novel Bradykinin-Potentiating Peptides and Three-Finger Toxins from Viper Venom: Combined NGS Venom Gland Transcriptomics and Quantitative Venom Proteomics of the Azemiops feae Viper" Biomedicines 8, no. 8: 249. https://doi.org/10.3390/biomedicines8080249