Targeting NF-κB Signaling by Calebin A, a Compound of Turmeric, in Multicellular Tumor Microenvironment: Potential Role of Apoptosis Induction in CRC Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Antibodies and Chemicals
2.2. Cells and Cell Culture Conditions
2.3. Experimental Study Design
2.4. Alginate Culture
2.5. Vitality and Proliferation
2.6. Colonosphere Formation and Invasion
2.7. Immunofluorescence
2.8. Western Blotting Investigations
2.9. DNA-Binding Assay
2.10. Statistical Evaluation
3. Results
3.1. Calebin A Suppresses Proliferation Promoted by TME Cultures in 3D-Alginate CRC Cells
3.2. Calebin A Suppresses Invasion and Colony Formation Ability, Promoted by TME in 3D-Alginate CRC Cells
3.3. Calebin A Decreases TME-Induced Activation and Nuclear Translocation of p65-NF-κB in CRC Cells
3.4. Calebin A Represses TME-Induced NF-κB Activation and NF-κB-Dependent Gene End-Products Involved in Cell Proliferation, Invasion, and Apoptosis in CRC Cells
3.5. Calebin A Reduces TME-Induced NF-κB-Dependent Anti-Apoptotic Gene Biomarkers in CRC Cells
3.6. Calebin A Disturbs the Direct Interaction of NF-κB with DNA in CRC Cells
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Buhrmann, C.; Shayan, P.; Banik, K.; Kunnumakkara, A.B.; Kubatka, P.; Koklesova, L.; Shakibaei, M. Targeting NF-κB Signaling by Calebin A, a Compound of Turmeric, in Multicellular Tumor Microenvironment: Potential Role of Apoptosis Induction in CRC Cells. Biomedicines 2020, 8, 236. https://doi.org/10.3390/biomedicines8080236
Buhrmann C, Shayan P, Banik K, Kunnumakkara AB, Kubatka P, Koklesova L, Shakibaei M. Targeting NF-κB Signaling by Calebin A, a Compound of Turmeric, in Multicellular Tumor Microenvironment: Potential Role of Apoptosis Induction in CRC Cells. Biomedicines. 2020; 8(8):236. https://doi.org/10.3390/biomedicines8080236
Chicago/Turabian StyleBuhrmann, Constanze, Parviz Shayan, Kishore Banik, Ajaikumar B. Kunnumakkara, Peter Kubatka, Lenka Koklesova, and Mehdi Shakibaei. 2020. "Targeting NF-κB Signaling by Calebin A, a Compound of Turmeric, in Multicellular Tumor Microenvironment: Potential Role of Apoptosis Induction in CRC Cells" Biomedicines 8, no. 8: 236. https://doi.org/10.3390/biomedicines8080236