Next Article in Journal
The Fabp4-Cre-Model is Insufficient to Study Hoxc9 Function in Adipose Tissue
Previous Article in Journal
Endothelial Dysfunction in Diabetes
Open AccessArticle

Hyaluronan Metabolism is Associated with DNA Repair Genes in Breast and Colorectal Cancer. Screening of Potential Progression Markers Using qPCR

1
Laboratorio de Microambiente Tumoral; Centro de Investigaciones Básicas y Aplicadas (CIBA), CIT NOBA, Universidad Nacional del Noroeste de la Pcia. de Bs. As. Consejo Nacional de Investigaciones Científicas y Técnicas (UNNOBA-CONICET), Junín B6000, Argentina
2
Laboratorio de Fisiopatología de la Hipófisis; Centro de Investigaciones Básicas y Aplicadas (CIBA), CIT NOBA, Universidad Nacional del Noroeste de la Pcia. de Bs. As. Consejo Nacional de Investigaciones Científicas y Técnicas (UNNOBA-CONICET), Junín B6000, Argentina
3
Hospital Interzonal General de Agudos Dr. Abraham F. Piñeyro, Junín B6000, Argentina
4
Clínica Centro, Junín B6000, Argentina
5
Instituto de Medicina Traslacional e Ingeniería Biomédica (IMTIB)-CONICET-Instituto Universitario del Hospital Italiano-Hospital Italiano Buenos Aires (HIBA), Buenos Aires C1199ACL, Argentina
*
Author to whom correspondence should be addressed.
Biomedicines 2020, 8(7), 183; https://doi.org/10.3390/biomedicines8070183
Received: 21 April 2020 / Revised: 1 June 2020 / Accepted: 3 June 2020 / Published: 29 June 2020
(This article belongs to the Section Tumor Cell Biology)
In this work, we compared mRNA levels of Hyaluronan (HA) metabolism members and BRCA genes, known to be involved in the tumoral process, between tumor and non-tumor adjacent tissue and its correlation with previously proposed biomarkers (ER, PR, HER2 and KI67) in order to assess their value as a progression biomarkers. We show alteration in HA metabolism in colorectal but not breast cancer. However, we found a decrease in Hyaluronidase 1 HYAL1 levels in the breast but not colorectal cancer. We also show lower HA levels in tumor compared with normal tissue that could indicate a possible influence of tumor on its surrounding “normal” tissue. In both breast and colorectal cancer, CD44 and BRCA2 showed a strong positive correlation. Besides, our results show first indicators that qPCR of the analyzed genes could be used as an easy and low cost procedure for the evaluation of molecular markers we propose here. View Full-Text
Keywords: tumor microenvironment; hyaluronan; hyaluronan metabolism; breast cancer; colorectal cancer; qPCR tumor microenvironment; hyaluronan; hyaluronan metabolism; breast cancer; colorectal cancer; qPCR
Show Figures

Figure 1

MDPI and ACS Style

Sevic, I.; Spinelli, F.M.; Vitale, D.L.; Icardi, A.; Romano, L.; Brandone, A.; Giannoni, P.; Cristina, C.; Bolontrade, M.F.; Alaniz, L. Hyaluronan Metabolism is Associated with DNA Repair Genes in Breast and Colorectal Cancer. Screening of Potential Progression Markers Using qPCR. Biomedicines 2020, 8, 183.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop