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Open AccessTechnical Note

Antibody-Free Labeling of Malaria-Derived Extracellular Vesicles Using Flow Cytometry

1
Faculty of Biochemistry, Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot 7610001, Israel
2
Flow Cytometry Unit, Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot 7610001, Israel
*
Authors to whom correspondence should be addressed.
Biomedicines 2020, 8(5), 98; https://doi.org/10.3390/biomedicines8050098
Received: 24 March 2020 / Revised: 19 April 2020 / Accepted: 21 April 2020 / Published: 27 April 2020
Extracellular vesicles (EVs) are cell-derived membrane-bound structures that are believed to play a major role in intercellular communication by allowing cells to exchange proteins and genetic cargo between them. In particular, pathogens, such as the malaria parasite Plasmodium (P.) falciparum, utilize EVs to promote their growth and to alter their host’s response. Thus, better characterization of these secreted organelles will enhance our understanding of the cellular processes that govern EVs’ biology and pathological functions. Here we present a method that utilizes a high-end flow cytometer system to characterize small EVs, i.e., with a diameter less than 200 nm. Using this method, we could evaluate different parasite-derived EV populations according to their distinct cargo by using antibody-free labeling. It further allows to closely monitor a sub-population of vesicles carrying parasitic DNA cargo. This ability paves the way to conducting a more ‘educated’ analysis of the various EV cargo components. View Full-Text
Keywords: malaria; extracellular vesicles; Plasmodium falciparum; flow cytometry malaria; extracellular vesicles; Plasmodium falciparum; flow cytometry
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MDPI and ACS Style

Dekel, E.; Abou Karam, P.; Ohana-Daniel, Y.; Biton, M.; Regev-Rudzki, N.; Porat, Z. Antibody-Free Labeling of Malaria-Derived Extracellular Vesicles Using Flow Cytometry. Biomedicines 2020, 8, 98.

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