Quality Assessment of Platelet-Rich Fibrin-Like Matrix Prepared from Whole Blood Samples after Extended Storage

The platelet-rich fibrin–like matrix (PRFM) is usually prepared onsite and immediately used for regenerative therapy. Nonetheless, to meet the clinical necessity of preserving the PRFM without quality deterioration, we developed a method for preparation of PRFMs from short-term-stored whole blood (WB) samples. In this study, to evaluate the practical expiration date of storage, we extended the storage time of WB samples from 2 to 7 days and assessed the quality of the resulting PRFMs. WB samples collected with acid-citrate-dextrose were stored with gentle agitation at ambient temperature. To prepare PRFMs, the stored WB samples were mixed with CaCl₂ in glass tubes and centrifuged. Fibrin fiber networks, CD41 and CD62P expression, and Platelet Derived Growth Factor-BB (PDGF-BB) levels were examined by scanning electron microscopy (SEM), flow cytometry, and an Enzyme-Linked ImmunoSorbent Assay (ELISA), respectively. Long-term storage had no significant effect on either blood cell counts or platelet functions tested. The resulting PRFMs were visually identical to freshly prepared ones. PDGF-BB levels did not markedly decrease in a time-dependent manner. However, fibrin fibers gradually became thinner after storage. Although the coagulation activity may diminish, we propose that PRFMs can be prepared—without evident loss of quality—from WB samples stored for up to 7 days by our previously developed method. View Full-Text

Keywords: platelets; platelet-rich fibrin; platelet-derived growth factor; fibrin fiber; storage

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MDPI and ACS Style

Kawabata, H.; Isobe, K.; Watanabe, T.; Okudera, T.; Nakamura, M.; Suzuki, M.; Ryu, J.; Kitamura, Y.; Okudera, H.; Okuda, K.; Nakata, K.; Kawase, T. Quality Assessment of Platelet-Rich Fibrin-Like Matrix Prepared from Whole Blood Samples after Extended Storage. Biomedicines 2017, 5, 57. https://doi.org/10.3390/biomedicines5030057

AMA Style

Kawabata H, Isobe K, Watanabe T, Okudera T, Nakamura M, Suzuki M, Ryu J, Kitamura Y, Okudera H, Okuda K, Nakata K, Kawase T. Quality Assessment of Platelet-Rich Fibrin-Like Matrix Prepared from Whole Blood Samples after Extended Storage. Biomedicines. 2017; 5(3):57. https://doi.org/10.3390/biomedicines5030057

Chicago/Turabian Style

Kawabata, Hideo, Kazushige Isobe, Taisuke Watanabe, Toshimitsu Okudera, Masayuki Nakamura, Masashi Suzuki, Jietsu Ryu, Yutaka Kitamura, Hajime Okudera, Kazuhiro Okuda, Koh Nakata, and Tomoyuki Kawase. 2017. "Quality Assessment of Platelet-Rich Fibrin-Like Matrix Prepared from Whole Blood Samples after Extended Storage" Biomedicines 5, no. 3: 57. https://doi.org/10.3390/biomedicines5030057

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Quality Assessment of Platelet-Rich Fibrin-Like Matrix Prepared from Whole Blood Samples after Extended Storage

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