Quality Assessment of Platelet-Rich Fibrin-Like Matrix Prepared from Whole Blood Samples after Extended Storage
by
Hideo Kawabata 1,
Kazushige Isobe 1,
Taisuke Watanabe 1,
Toshimitsu Okudera 1,
Masayuki Nakamura 1,
Masashi Suzuki 1,
Jietsu Ryu 1,
Yutaka Kitamura 1,
Hajime Okudera 1,
Kazuhiro Okuda 2,
Koh Nakata 3 and
Tomoyuki Kawase 4,*
Hideo Kawabata 1,
Kazushige Isobe 1,
Taisuke Watanabe 1,
Toshimitsu Okudera 1,
Masayuki Nakamura 1,
Masashi Suzuki 1,
Jietsu Ryu 1,
Yutaka Kitamura 1,
Hajime Okudera 1,
Kazuhiro Okuda 2,
Koh Nakata 3 and
Tomoyuki Kawase 4,*
1
Tokyo Plastic Dental Society, Kita-ku, Tokyo 1140002, Japan
2
Division of Periodontology, Institute of Medicine and Dentistry, Niigata University, Niigata 9518514, Japan
3
Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, Niigata 9518520, Japan
4
Division of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University, Niigata 9518514, Japan
*
Author to whom correspondence should be addressed.
Biomedicines 2017, 5(3), 57; https://doi.org/10.3390/biomedicines5030057
Received: 20 August 2017 / Revised: 11 September 2017 / Accepted: 14 September 2017 / Published: 18 September 2017
(This article belongs to the Special Issue Bone Cells and Related Interactions)
The platelet-rich fibrin–like matrix (PRFM) is usually prepared onsite and immediately used for regenerative therapy. Nonetheless, to meet the clinical necessity of preserving the PRFM without quality deterioration, we developed a method for preparation of PRFMs from short-term-stored whole blood (WB) samples. In this study, to evaluate the practical expiration date of storage, we extended the storage time of WB samples from 2 to 7 days and assessed the quality of the resulting PRFMs. WB samples collected with acid-citrate-dextrose were stored with gentle agitation at ambient temperature. To prepare PRFMs, the stored WB samples were mixed with CaCl2 in glass tubes and centrifuged. Fibrin fiber networks, CD41 and CD62P expression, and Platelet Derived Growth Factor-BB (PDGF-BB) levels were examined by scanning electron microscopy (SEM), flow cytometry, and an Enzyme-Linked ImmunoSorbent Assay (ELISA), respectively. Long-term storage had no significant effect on either blood cell counts or platelet functions tested. The resulting PRFMs were visually identical to freshly prepared ones. PDGF-BB levels did not markedly decrease in a time-dependent manner. However, fibrin fibers gradually became thinner after storage. Although the coagulation activity may diminish, we propose that PRFMs can be prepared—without evident loss of quality—from WB samples stored for up to 7 days by our previously developed method.
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MDPI and ACS Style
Kawabata, H.; Isobe, K.; Watanabe, T.; Okudera, T.; Nakamura, M.; Suzuki, M.; Ryu, J.; Kitamura, Y.; Okudera, H.; Okuda, K.; Nakata, K.; Kawase, T. Quality Assessment of Platelet-Rich Fibrin-Like Matrix Prepared from Whole Blood Samples after Extended Storage. Biomedicines 2017, 5, 57.
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