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Biomedicines 2017, 5(2), 32;

Biocompatibility Analyses of Al2O3-Treated Titanium Plates Tested with Osteocyte and Fibroblast Cell Lines

Dipartimento di Scienze Biomediche, Metaboliche e Neuroscienze, Sezione di Morfologia Umana, Università di Modena e R.E, 41124 Modena, Italy
Author to whom correspondence should be addressed.
Received: 9 April 2017 / Revised: 31 May 2017 / Accepted: 13 June 2017 / Published: 16 June 2017
(This article belongs to the Special Issue Bone Cells and Related Interactions)
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Osseointegration of a titanium implant is still an issue in dental/orthopedic implants durable over time. The good integration of these implants is mainly due to their surface and topography. We obtained an innovative titanium surface by shooting different-in-size particles of Al2O3 against the titanium scaffolds which seems to be ideal for bone integration. To corroborate that, we used two different cell lines: MLO-Y4 (murine osteocytes) and 293 (human fibroblasts) and tested the titanium scaffolds untreated and treated (i.e., Al2O3 shot-peened titanium surfaces). Distribution, density, and expression of adhesion molecules (fibronectin and vitronectin) were evaluated under scanning electron microscope (SEM) and confocal microscope (CM). DAPI and fluorochrome-conjugated antibodies were used to highlight nuclei, fibronectin, and vitronectin, under CM; cell distribution was analyzed after gold-palladium sputtering of samples by SEM. The engineered biomaterial surfaces showed under SEM irregular morphology displaying variously-shaped spicules. Both SEM and CM observations showed better outcome in terms of cell adhesion and distribution in treated titanium surfaces with respect to the untreated ones. The results obtained clearly showed that this kind of surface-treated titanium, used to manufacture devices for dental implantology: (i) is very suitable for cell colonization, essential prerequisite for the best osseointegration, and (ii) represents an excellent solution for the development of further engineered implants with the target to obtain recovery of stable dental function over time. View Full-Text
Keywords: osseointegration; titanium plate; osteocyte; biocompatibility; implants osseointegration; titanium plate; osteocyte; biocompatibility; implants

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Smargiassi, A.; Bertacchini, J.; Checchi, M.; Cavani, F.; Ferretti, M.; Palumbo, C. Biocompatibility Analyses of Al2O3-Treated Titanium Plates Tested with Osteocyte and Fibroblast Cell Lines. Biomedicines 2017, 5, 32.

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