Next Article in Journal
Unlocking the NF-κB Conundrum: Embracing Complexity to Achieve Specificity
Next Article in Special Issue
Toward the Selection of Cell Targeting Aptamers with Extended Biological Functionalities to Facilitate Endosomal Escape of Cargoes
Previous Article in Journal
Hitting the Holy Grail of Hematopoietic Cell Transplantation with Naive T-Cell Depleted Allografts—Graft Engineered Hematopoietic Stem Cell Transplant
Previous Article in Special Issue
Aptamer-siRNA Chimeras: Discovery, Progress, and Future Prospects
Open AccessReview

Aptamer Cell-Based Selection: Overview and Advances

Istituto di Endocrinologia ed Oncologia Sperimentale “G. Salvatore”, CNR, Naples 80100, Italy
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Biomedicines 2017, 5(3), 49; https://doi.org/10.3390/biomedicines5030049
Received: 12 July 2017 / Revised: 3 August 2017 / Accepted: 8 August 2017 / Published: 14 August 2017
(This article belongs to the Special Issue Engineering Aptamers for Biomedical Applications)
Aptamers are high affinity single-stranded DNA/RNA molecules, produced by a combinatorial procedure named SELEX (Systematic Evolution of Ligands by Exponential enrichment), that are emerging as promising diagnostic and therapeutic tools. Among selection strategies, procedures using living cells as complex targets (referred as “cell-SELEX”) have been developed as an effective mean to generate aptamers for heavily modified cell surface proteins, assuring the binding of the target in its native conformation. Here we give an up-to-date overview on cell-SELEX technology, discussing the most recent advances with a particular focus on cancer cell targeting. Examples of the different protocol applications and post-SELEX strategies will be briefly outlined. View Full-Text
Keywords: aptamer; cell- Systematic Evolution of Ligands by Exponential enrichment; cell targeting aptamer; cell- Systematic Evolution of Ligands by Exponential enrichment; cell targeting
Show Figures

Graphical abstract

MDPI and ACS Style

Catuogno, S.; Esposito, C.L. Aptamer Cell-Based Selection: Overview and Advances. Biomedicines 2017, 5, 49. https://doi.org/10.3390/biomedicines5030049

AMA Style

Catuogno S, Esposito CL. Aptamer Cell-Based Selection: Overview and Advances. Biomedicines. 2017; 5(3):49. https://doi.org/10.3390/biomedicines5030049

Chicago/Turabian Style

Catuogno, Silvia; Esposito, Carla Lucia. 2017. "Aptamer Cell-Based Selection: Overview and Advances" Biomedicines 5, no. 3: 49. https://doi.org/10.3390/biomedicines5030049

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop