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Biomedicines
Volume 14
Issue 2
10.3390/biomedicines14020319
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Adverse Histopathological Features in Colorectal Cancer Associated with KRAS rs61764370 SNP: A Preliminary Study

by
Tradian Ciprian Berisha
1,2,†,
Mihai Gabriel Cucu
3,4,†,
Alexandru Calotă-Dobrescu
4,
Simona Serban Sosoi
3,
Ana-Maria Ciurea
2,5,
Alina Maria Mehedințeanu
2,
Puiu Olivian Stovicek
2,6,
Ramona Adriana Schenker
2,
Cecil Sorin Mirea
7,
Monica-Laura Cara
2,8,*,
Florin Burada
3,4,*,‡ and
Michael Schenker
2,5,‡
1
Doctoral School, University of Medicine and Pharmacy Craiova, 200349 Craiova, Romania
2
Sf. Nectarie Oncology Center, 200347 Craiova, Romania
3
Human Genomics Laboratory, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania
4
Regional Centre of Medical Genetics Dolj, Emergency Clinical County Hospital Craiova, 200642 Craiova, Romania
5
Department of Oncology, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania
6
Department of Pharmacology, Faculty of Nursing, Târgu Jiu Subsidiary, “Titu Maiorescu” University, 040441 Bucharest, Romania
7
Department of Surgical Semiology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania
8
Department of Public Health, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
‡
These authors also contributed equally to this work.
Biomedicines 2026, 14(2), 319; https://doi.org/10.3390/biomedicines14020319
Submission received: 30 December 2025 / Revised: 28 January 2026 / Accepted: 28 January 2026 / Published: 30 January 2026
(This article belongs to the Special Issue Recent Advances in Gastrointestinal Cancers: From Microbiota Modulation to New Therapeutic Approaches—2nd Edition)
Versions Notes

Abstract

Background/Objectives: The KRAS rs61764370 T>G single-nucleotide polymorphism (SNP), located in a let-7 microRNA binding site within the 3′ untranslated region (3′UTR) of the KRAS gene, may modulate tumor aggressiveness by altering post-transcriptional gene regulation. This study evaluated its association with adverse histopathological features in colorectal cancer (CRC). Methods: A preliminary study on 83 CRC patients carrying either the TT (wild-type, n = 64) or TG (heterozygous, n = 19) genotype was analyzed. Clinicopathological variables included patient sex, tumor location, American Joint Committee on Cancer (AJCC) staging system, histological grade, perineural invasion (PNI), and lymphovascular invasion (LVI). A composite “tumor aggressiveness” score was defined based on the presence of Grade 3 differentiation, LVI, and/or PNI. Group comparisons were performed using the Chi-square test or Fisher’s exact test, as appropriate. Results: No statistically significant differences were observed in sex (p = 0.689), tumor location (p = 0.781), or stage at diagnosis (p = 0.812). Poorly differentiated tumors (Grade 3) were present in 20.3% of TT patients and absent in TG carriers (p = 0.06), while low-grade tumors (Grade 1) were more prevalent among TG patients (47.4%) compared to TT (29.7%). The composite high-aggressiveness score was lower in TG (36.8%) than in TT (48.4%), while co-occurrence of PNI and LVI was similar in both groups (~26%). Conclusions: Although no significant associations were identified, TG carriers showed a tendency toward lower-grade, less aggressive tumors. Given the limited sample size, these findings should be interpreted with caution, necessitating larger cohorts in order to validate results.
Keywords: colorectal cancer; KRAS; rs61764370; LCS6; SNP; microRNA; aggressiveness; biomarker colorectal cancer; KRAS; rs61764370; LCS6; SNP; microRNA; aggressiveness; biomarker

Share and Cite

MDPI and ACS Style

Berisha, T.C.; Cucu, M.G.; Calotă-Dobrescu, A.; Sosoi, S.S.; Ciurea, A.-M.; Mehedințeanu, A.M.; Stovicek, P.O.; Schenker, R.A.; Mirea, C.S.; Cara, M.-L.; et al. Adverse Histopathological Features in Colorectal Cancer Associated with KRAS rs61764370 SNP: A Preliminary Study. Biomedicines 2026, 14, 319. https://doi.org/10.3390/biomedicines14020319

AMA Style

Berisha TC, Cucu MG, Calotă-Dobrescu A, Sosoi SS, Ciurea A-M, Mehedințeanu AM, Stovicek PO, Schenker RA, Mirea CS, Cara M-L, et al. Adverse Histopathological Features in Colorectal Cancer Associated with KRAS rs61764370 SNP: A Preliminary Study. Biomedicines. 2026; 14(2):319. https://doi.org/10.3390/biomedicines14020319

Chicago/Turabian Style

Berisha, Tradian Ciprian, Mihai Gabriel Cucu, Alexandru Calotă-Dobrescu, Simona Serban Sosoi, Ana-Maria Ciurea, Alina Maria Mehedințeanu, Puiu Olivian Stovicek, Ramona Adriana Schenker, Cecil Sorin Mirea, Monica-Laura Cara, and et al. 2026. "Adverse Histopathological Features in Colorectal Cancer Associated with KRAS rs61764370 SNP: A Preliminary Study" Biomedicines 14, no. 2: 319. https://doi.org/10.3390/biomedicines14020319

APA Style

Berisha, T. C., Cucu, M. G., Calotă-Dobrescu, A., Sosoi, S. S., Ciurea, A.-M., Mehedințeanu, A. M., Stovicek, P. O., Schenker, R. A., Mirea, C. S., Cara, M.-L., Burada, F., & Schenker, M. (2026). Adverse Histopathological Features in Colorectal Cancer Associated with KRAS rs61764370 SNP: A Preliminary Study. Biomedicines, 14(2), 319. https://doi.org/10.3390/biomedicines14020319

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