In Vivo Study on Doxycycline Protective Mechanisms during Myocardial Ischemia Injury in Rats
Abstract
1. Introduction
2. Materials and Methods
2.1. Experimental Animals and Ethical Statement
2.2. Drug Preparation
2.3. Myocardial Infarction Surgery
2.4. Transthoracic Echocardiography
2.5. Evans Blue Followed by 2,3,5-Triphenyltetrazolium Chloride Staining of Hearts
2.6. Hematoxylin/Eosin Staining of Hearts
2.7. Preparation of Heart Tissue Homogenate
2.8. Quantitative Measurement of Cardiac Troponin I Protein
2.9. Measurement of LDH Activity
2.10. Assessment of Cytotoxicity Level in Rat Hearts
2.11. Matrix Metalloproteinase Activity Assay
2.12. Measurement of Total Matrix Metalloproteinase 2 and 9 Concentration in Heart Homogenates
2.13. Measurement of MLC1 Concentration
2.14. Statistical Analysis
3. Results
3.1. Procedures and Mortality
3.2. Doxycycline Protects from Heart Tissue Damage
3.3. Doxycycline Protects from I/R Associated Impairment of Left Ventricular Function
3.4. Effect of Doxy on MMPs Activity and Concentration
3.5. Administration of Doxy Protects from Cardiac Contractile Protein’s Degradation Due to I/R Injury
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Echocardiographic Parameters | Heart Rate (bpm) | Cardiac Output (mL/min) | Stroke Volume (µL) | Fractional Shortening (%) | Ejection Fraction (%) | Enddiastolic Volume (µL) | Endsystolic Volume (µL) | |
---|---|---|---|---|---|---|---|---|
baseline | sham | 386.7 ± 20.2 | 64.7 ± 12 | 206 ± 27.4 | 48.2 ± 5.7 | 87.1 ± 2.5 | 176.6 ± 36 | 36.77 ± 8 |
I/R | 349.3 ± 30.3 | 68 ± 12 | 204.4 ± 21.6 | 46.2 ± 10.9 | 79.4 ± 11.5 | 159.8 ± 46.7 | 26.77 ± 12.6 | |
I/R Doxy | 384.2 ± 48.6 | 83.6 ± 31 | 211.1 ± 55.4 | 51.32 ± 3.7 | 83.3 ± 11.8 | 174.9 ± 42.3 | 32.49 ± 17.6 | |
7 days | sham | 408.7 ± 16.3 | 80.9 ± 9.7 | 212.5 ± 30 | 48.53 ± 5.5 | 95.11 ± 5.7 | 176.5 ± 17 | 38 ± 5.6 |
I/R | 378.3 ± 35 | 51.73 ± 15.6 | 140 ± 25.1 | 27.56 ± 7.5 | 63.59 ± 12.9 | 290 ± 99.8 | 60.93 ± 13.77 | |
I/R Doxy | 384.4 ± 23 | 89.24 ± 18.4 | 201.4 ± 45.2 | 49.02 ± 12.9 | 87.69 ± 6 | 219 ± 54.4 | 26.03 ± 9.8 | |
14 days | sham | 369.3 ±18 | 87.18 ± 17.5 | 220.9 ± 29.3 | 50.64 ± 2 | 93.21 ± 2 | 184.7 ± 15 | 28.78 ±7.7 |
I/R | 378 ± 34.9 | 54.71 ± 13.1 | 111.6 ± 44 | 35.06 ± 8.8 | 64.99 ± 12.2 | 280.3 ± 78 | 55.7, ± 32.9 | |
I/R Doxy | 391.2 ± 43.5 | 79.17 ± 11.4 | 194.6 ± 36.3 | 50.51 ± 7.6 | 86.38 ± 5 | 219.4 ± 54 | 31.93 ±11.5 | |
p | sham vs. I/R | 0.328/0.313/>0.99 | 0.971/0.049 */0.009 * | 0.996/0.032 */0.006 * | 0.934/0.018 */0.012 * | 0.57/0.002 */0.002 * | 0.844/0.006 */0.016 * | 0.566/0.033 */0.002 * |
sham vs. I/R Doxy | 0.995/0.502/0.201 | 0.446/0.756/0.49 | 0.983/0.9/0.636 | 0.864/0.997/0.1 | 0.882/0.582/0.573 | 0.999/0.39/0.827 | 0.907/0.36/0.924 | |
I/R vs. I/R Doxy | 0.266/0.931/0.791 | 0.427/0.004 */0.037 * | 0.947/0.033 */0.012 * | 0.556/0.005 */0.004 * | 0.812/0.004 */0.004 * | 0.826/0.037 */0.020 * | 0.767/0.001 */0.001 * |
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Krzywonos-Zawadzka, A.; Olejnik, A.; Sawicki, G.; Bil-Lula, I. In Vivo Study on Doxycycline Protective Mechanisms during Myocardial Ischemia Injury in Rats. Biomedicines 2024, 12, 634. https://doi.org/10.3390/biomedicines12030634
Krzywonos-Zawadzka A, Olejnik A, Sawicki G, Bil-Lula I. In Vivo Study on Doxycycline Protective Mechanisms during Myocardial Ischemia Injury in Rats. Biomedicines. 2024; 12(3):634. https://doi.org/10.3390/biomedicines12030634
Chicago/Turabian StyleKrzywonos-Zawadzka, Anna, Agnieszka Olejnik, Grzegorz Sawicki, and Iwona Bil-Lula. 2024. "In Vivo Study on Doxycycline Protective Mechanisms during Myocardial Ischemia Injury in Rats" Biomedicines 12, no. 3: 634. https://doi.org/10.3390/biomedicines12030634
APA StyleKrzywonos-Zawadzka, A., Olejnik, A., Sawicki, G., & Bil-Lula, I. (2024). In Vivo Study on Doxycycline Protective Mechanisms during Myocardial Ischemia Injury in Rats. Biomedicines, 12(3), 634. https://doi.org/10.3390/biomedicines12030634