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Article

Evaluation of the Effectiveness of an Innovative Polycomponent Formulation on Adult and Aged Human Dermal Fibroblasts

by
Francesca Rosaria Augello
1,†,
Francesca Lombardi
1,†,
Serena Artone
2,
Alessia Ciafarone
3,
Serena Altamura
2,
Luisa Di Marzio
4,
Maria Grazia Cifone
1,
Paola Palumbo
1,
Maurizio Giuliani
1,5 and
Benedetta Cinque
1,*
1
Department of Life, Health & Environmental Sciences, University of L’Aquila, 67100 L’Aquila, Italy
2
PhD School in Medicine and Public Health, Department of Life, Health and Environmental Sciences, University of L’Aquila, 67100 L’Aquila, Italy
3
PhD School in Health & Environmental Sciences, Department of Life, Health and Environmental Sciences, University of L’Aquila, 67100 L’Aquila, Italy
4
Department of Pharmacy, University of Chieti—Pescara “G. D’Annunzio”, 66100 Chieti, Italy
5
Unit of Plastic and Reconstructive Surgery, Casa Di Cura Di Lorenzo SrL, Via Vittorio Veneto 37, 67051 Avezzano, Italy
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Biomedicines 2023, 11(9), 2410; https://doi.org/10.3390/biomedicines11092410
Submission received: 3 August 2023 / Revised: 24 August 2023 / Accepted: 26 August 2023 / Published: 28 August 2023
(This article belongs to the Special Issue Biomedicines: 10th Anniversary)
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Abstract

Skin aging is a dynamic process that determines structural alterations in ECM and reduction in dermal fibroblasts. The recent availability on the market of an innovative polycomponent formulation (KARISMA Rh Collagen® FACE, K) containing noncrosslinked high-molecular-weight hyaluronic acid (HMW-HA), a human recombinant polypeptide of collagen-1 alpha chain, and carboxymethyl cellulose (CMC), attracted our scientific interest in evaluating its biomolecular effects on human dermal adult and aged fibroblasts. After treatment with increasing K concentrations, cell proliferation, collagen I, prolyl 4-hydroxylase (P4HA1), an essential protein in collagen biosynthesis, and α-SMA levels were assessed. The fibroblast contractility, TGF-β1 levels, and oxidative stress markers were also evaluated. K formulation exposure led to a significant and dose-dependent increase in the proliferation and migration of adult fibroblasts. Of note, the K exposure counteracted the H2O2-induced aging by promoting cell proliferation, reducing β-galactosidase activity, and neutralizing the aging-associated oxidative damage. Moreover, an increase in collagen I, P4HA1, α-SMA, TGF-β1 levels, and improved contractility of adult and aged fibroblasts were observed after treatment. Overall, our results show evidence that the K treatment is efficacious in improving biological functions in adult fibroblasts and suppressing the biomolecular events associated with H2O2-induced cellular aging, thus supporting the regenerative and bio-revitalizing action of the K formulation helpful in preventing or treating skin aging.
Keywords: polycomponent formulation; skin aging; dermal fibroblast; human recombinant polypeptide of collagen-1 α chain; TGF-β1; oxidative stress polycomponent formulation; skin aging; dermal fibroblast; human recombinant polypeptide of collagen-1 α chain; TGF-β1; oxidative stress
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MDPI and ACS Style

Augello, F.R.; Lombardi, F.; Artone, S.; Ciafarone, A.; Altamura, S.; Di Marzio, L.; Cifone, M.G.; Palumbo, P.; Giuliani, M.; Cinque, B. Evaluation of the Effectiveness of an Innovative Polycomponent Formulation on Adult and Aged Human Dermal Fibroblasts. Biomedicines 2023, 11, 2410. https://doi.org/10.3390/biomedicines11092410

AMA Style

Augello FR, Lombardi F, Artone S, Ciafarone A, Altamura S, Di Marzio L, Cifone MG, Palumbo P, Giuliani M, Cinque B. Evaluation of the Effectiveness of an Innovative Polycomponent Formulation on Adult and Aged Human Dermal Fibroblasts. Biomedicines. 2023; 11(9):2410. https://doi.org/10.3390/biomedicines11092410

Chicago/Turabian Style

Augello, Francesca Rosaria, Francesca Lombardi, Serena Artone, Alessia Ciafarone, Serena Altamura, Luisa Di Marzio, Maria Grazia Cifone, Paola Palumbo, Maurizio Giuliani, and Benedetta Cinque. 2023. "Evaluation of the Effectiveness of an Innovative Polycomponent Formulation on Adult and Aged Human Dermal Fibroblasts" Biomedicines 11, no. 9: 2410. https://doi.org/10.3390/biomedicines11092410

APA Style

Augello, F. R., Lombardi, F., Artone, S., Ciafarone, A., Altamura, S., Di Marzio, L., Cifone, M. G., Palumbo, P., Giuliani, M., & Cinque, B. (2023). Evaluation of the Effectiveness of an Innovative Polycomponent Formulation on Adult and Aged Human Dermal Fibroblasts. Biomedicines, 11(9), 2410. https://doi.org/10.3390/biomedicines11092410

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