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Biomedicines
Volume 10
Issue 12
10.3390/biomedicines10123143
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Brief Report

Cardiac Mesenchymal Stem Cell-like Cells Derived from a Young Patient with Bicuspid Aortic Valve Disease Have a Prematurely Aged Phenotype

by
Rachel A. Oldershaw
1,*,
Gavin Richardson
2,
Phillippa Carling
2,
W. Andrew Owens
2,3,
David J. Lundy
4 and
Annette Meeson
2
1
Department of Musculoskeletal and Ageing Science, Institute of Life Course and Medical Sciences, Faculty of Health and Life Sciences, University of Liverpool, William Henry Duncan Building, 6 West Derby Street, Liverpool L7 8TX, UK
2
Newcastle University Bioscience Institute, Newcastle University, International Centre for Life, Central Parkway, Newcastle upon Tyne NE1 3BZ, UK
3
Department of Cardiothoracic Surgery, South Tees Hospitals NHS Foundation Trust, Middlesbrough TS4 3BW, UK
4
Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei 110, Taiwan
*
Author to whom correspondence should be addressed.
Biomedicines 2022, 10(12), 3143; https://doi.org/10.3390/biomedicines10123143
Submission received: 4 November 2022 / Revised: 25 November 2022 / Accepted: 28 November 2022 / Published: 6 December 2022
(This article belongs to the Special Issue Cardiomyopathy and Vasculopathy: From Molecular Basis to Advanced Therapy)
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Abstract

There is significant interest in the role of stem cells in cardiac regeneration, and yet little is known about how cardiac disease progression affects native cardiac stem cells in the human heart. In this brief report, cardiac mesenchymal stem cell-like cells (CMSCLC) from the right atria of a 21-year-old female patient with a bicuspid aortic valve and aortic stenosis (referred to as biscuspid aortic valve disease BAVD-CMSCLC), were compared with those of a 78-year-old female patient undergoing coronary artery bypass surgery (referred to as coronary artery disease CAD-CMSCLC). Cells were analyzed for expression of MSC markers, ability to form CFU-Fs, metabolic activity, cell cycle kinetics, expression of NANOG and p16, and telomere length. The cardiac-derived cells expressed MSC markers and were able to form CFU-Fs, with higher rate of formation in CAD-CMSCLCs. BAVD-CMSCLCs did not display normal MSC morphology, had a much lower cell doubling rate, and were less metabolically active than CAD-CMSCLCs. Cell cycle analysis revealed a population of BAVD-CMSCLC in G2/M phase, whereas the bulk of CAD-CMSCLC were in the G0/G1 phase. BAVD-CMSCLC had lower expression of NANOG and shorter telomere lengths, but higher expression of p16 compared with the CAD-CMSCLC. In conclusion, BAVD-CMSCLC have a prematurely aged phenotype compared with CAD-CMSCLC, despite originating from a younger patient.
Keywords: cardiac mesenchymal stem cell-like cells; bicuspid aortic valve disease; coronary artery disease; mesenchymal stem cells; ageing; senescence cardiac mesenchymal stem cell-like cells; bicuspid aortic valve disease; coronary artery disease; mesenchymal stem cells; ageing; senescence

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MDPI and ACS Style

Oldershaw, R.A.; Richardson, G.; Carling, P.; Owens, W.A.; Lundy, D.J.; Meeson, A. Cardiac Mesenchymal Stem Cell-like Cells Derived from a Young Patient with Bicuspid Aortic Valve Disease Have a Prematurely Aged Phenotype. Biomedicines 2022, 10, 3143. https://doi.org/10.3390/biomedicines10123143

AMA Style

Oldershaw RA, Richardson G, Carling P, Owens WA, Lundy DJ, Meeson A. Cardiac Mesenchymal Stem Cell-like Cells Derived from a Young Patient with Bicuspid Aortic Valve Disease Have a Prematurely Aged Phenotype. Biomedicines. 2022; 10(12):3143. https://doi.org/10.3390/biomedicines10123143

Chicago/Turabian Style

Oldershaw, Rachel A., Gavin Richardson, Phillippa Carling, W. Andrew Owens, David J. Lundy, and Annette Meeson. 2022. "Cardiac Mesenchymal Stem Cell-like Cells Derived from a Young Patient with Bicuspid Aortic Valve Disease Have a Prematurely Aged Phenotype" Biomedicines 10, no. 12: 3143. https://doi.org/10.3390/biomedicines10123143

APA Style

Oldershaw, R. A., Richardson, G., Carling, P., Owens, W. A., Lundy, D. J., & Meeson, A. (2022). Cardiac Mesenchymal Stem Cell-like Cells Derived from a Young Patient with Bicuspid Aortic Valve Disease Have a Prematurely Aged Phenotype. Biomedicines, 10(12), 3143. https://doi.org/10.3390/biomedicines10123143

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