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Article

Glucose Biosensor Based on Dendritic Gold Nanostructures Electrodeposited on Graphite Electrode by Different Electrochemical Methods

1
NanoTechnas—Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, LT-03225 Vilnius, Lithuania
2
Department of Immunology, State Research Institute Centre for Innovative Medicine, Santariskiu 5, LT-08406 Vilnius, Lithuania
3
Department of Physical Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania
*
Author to whom correspondence should be addressed.
Academic Editors: Xudong Wang and Hongshang Peng
Chemosensors 2021, 9(8), 188; https://doi.org/10.3390/chemosensors9080188
Received: 22 June 2021 / Revised: 13 July 2021 / Accepted: 19 July 2021 / Published: 22 July 2021
In this research, we have demonstrated a one-step electrochemical deposition of dendritic gold nanostructures (DGNs) on a graphite rod (GR) electrode without any template, seeds, surfactants, or stabilizers. Three electrochemical methods, namely, constant potential amperometry (CPA), pulse amperometry, and differential pulse voltammetry, were used for DGN synthesis on GR electrode and further application in enzymatic glucose biosensors. Formed gold nanostructures, including DGNs, were characterized by a field emission scanning electron microscopy. The optimal concentration of HAuCl4 (6.0 mmol L−1), duration of DGNs synthesis (400 s), electrodeposition potential (−0.4 V), and the best electrochemical method (CPA) were determined experimentally. Then the enzyme, glucose oxidase, was adsorbed on the surface of DGNs and covalently cross-linked with glutaraldehyde vapor. The enzymatic glucose biosensor based on DGNs electrodeposited at optimal conditions and modified with glucose oxidase showed a quick response (less than 3 s), a high saturation current (291 μA), appropriate linear range (up to 9.97 mmol L−1 of glucose, R2 = 0.9994), good repeatability (RSD 2.4, 2.2 and 1.5% for 2, 30, 97 mmol L−1 of glucose), low limit of detection (0.059 mmol L−1, S/N = 3) and good stability. Additionally, this biosensor could be successfully applied for glucose determination in real samples with good accuracy. These results proved the principle of enzymatic glucose biosensor development based on DGNs as the basis for further investigations. View Full-Text
Keywords: dendritic gold nanostructures; constant potential amperometry; pulse amperometry; differential pulse voltammetry; glucose oxidase; glucose biosensor dendritic gold nanostructures; constant potential amperometry; pulse amperometry; differential pulse voltammetry; glucose oxidase; glucose biosensor
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MDPI and ACS Style

Ramanaviciene, A.; German, N.; Kausaite-Minkstimiene, A.; Ramanavicius, A. Glucose Biosensor Based on Dendritic Gold Nanostructures Electrodeposited on Graphite Electrode by Different Electrochemical Methods. Chemosensors 2021, 9, 188. https://doi.org/10.3390/chemosensors9080188

AMA Style

Ramanaviciene A, German N, Kausaite-Minkstimiene A, Ramanavicius A. Glucose Biosensor Based on Dendritic Gold Nanostructures Electrodeposited on Graphite Electrode by Different Electrochemical Methods. Chemosensors. 2021; 9(8):188. https://doi.org/10.3390/chemosensors9080188

Chicago/Turabian Style

Ramanaviciene, Almira, Natalija German, Asta Kausaite-Minkstimiene, and Arunas Ramanavicius. 2021. "Glucose Biosensor Based on Dendritic Gold Nanostructures Electrodeposited on Graphite Electrode by Different Electrochemical Methods" Chemosensors 9, no. 8: 188. https://doi.org/10.3390/chemosensors9080188

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