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Open AccessArticle

Development of a NanoMIPs-SPR-Based Sensor for β-Lactoglobulin Detection

1
Advanced Diagnostics and Sensors Group, Cranfield University, Cranfield, Bedfordshire MK43 0AL, UK
2
School of Chemical Engineering and Analytical Science, University of Manchester, Oxford Road, Manchester M13 9PL, UK
3
Safety and Environmental Assurance Centre, Colworth Science Park, Unilever plc, Sharnbrook, Bedford MK44 1LQ, UK
*
Authors to whom correspondence should be addressed.
Current address: Department of Health Technology, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark.
Chemosensors 2020, 8(4), 94; https://doi.org/10.3390/chemosensors8040094
Received: 2 August 2020 / Revised: 23 September 2020 / Accepted: 26 September 2020 / Published: 1 October 2020
(This article belongs to the Special Issue Sensors for Allergen Detection)
Food manufacturers are aiming to manage the levels of cross-contamination of allergens within food processing plants and ultimately move away from precautionary labelling. Hence, the need for rapid methods to detect allergens cross-contamination. A sensitive and selective label-free nanoMIPs based sensor was developed and tested for the detection of β-lactoglobulin (BLG). NanoMIPs were synthesized using solid-phase synthesis and appeared as spherical nanoparticles with sizes ranging from 264–294 nm, using dynamic light scattering (DLS). The nanoMIPs were functionalized with amine groups and attached to the surface of the SPR gold chip via amine-coupling protocol. The SPR nanoMIPs-based sensor demonstrated a detection limit of 3 ng mL−1 (211 pM) over a linear range of 1–5000 ng mL−1, with binding affinity of 7.0 × 10−8 M and specificity towards BLG. With further testing and final optimization, the developed nanosensor can be integrated on-line or at-line cleaning-in-place (CIP) wash systems, allowing to effectively monitor milk protein allergens as a rapid, point-of-source methodology. View Full-Text
Keywords: β-lactoglobulin (BLG); molecularly imprinted polymer (MIP); nanoparticles; surface plasmon resonance; food safety; allergen management β-lactoglobulin (BLG); molecularly imprinted polymer (MIP); nanoparticles; surface plasmon resonance; food safety; allergen management
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MDPI and ACS Style

D’Aurelio, R.; Ashley, J.; Rodgers, T.L.; Trinh, L.; Temblay, J.; Pleasants, M.; Tothill, I.E. Development of a NanoMIPs-SPR-Based Sensor for β-Lactoglobulin Detection. Chemosensors 2020, 8, 94.

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