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Open AccessArticle

Sensitive Electrochemical Detection of Tryptophan Using a Hemin/G-Quadruplex Aptasensor

1
Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Korea
2
Electroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar 47416-95447, Iran
*
Author to whom correspondence should be addressed.
Chemosensors 2020, 8(4), 100; https://doi.org/10.3390/chemosensors8040100
Received: 13 September 2020 / Revised: 12 October 2020 / Accepted: 12 October 2020 / Published: 15 October 2020
In this study, we design an electrochemical aptasensor with an enzyme-free amplification method to detect tryptophan (Trp). For the amplified electrochemical signal, the screen-printed electrode was modified with dendritic gold nanostructures (DGNs)/magnetic double-charged diazoniabicyclo [2.2.2] octane dichloride silica hybrid (Fe3O4@SiO2/DABCO) to increase the surface area as well as electrical conductivity, and the hemin/G-quadruplex aptamer was immobilized. The presence of Trp improved the catalytic characteristic of hemin/G-quadruplex structure, which resulted in the efficient catalysis of the H2O2 reduction. As the concentration of Trp increased, the intensity of H2O2 reduction signal increased, and Trp was measured in the range of 0.007–200 nM with a detection limit of 0.002 nM. Compared with previous models, our sensor displayed higher detection sensitivity and specificity for Trp. Furthermore, we demonstrated that the proposed aptasensor successfully determined Trp in human serum samples, thereby proving its practical applicability.
Keywords: magnetic nanocomposite; aptamer; tryptophan; G-quadruplex; electrochemical sensor; dendritic gold nanostructures magnetic nanocomposite; aptamer; tryptophan; G-quadruplex; electrochemical sensor; dendritic gold nanostructures
MDPI and ACS Style

Hashkavayi, A.B.; Raoof, J.B.; Park, K.S. Sensitive Electrochemical Detection of Tryptophan Using a Hemin/G-Quadruplex Aptasensor. Chemosensors 2020, 8, 100.

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