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Article

Development of Novel and Highly Specific ssDNA-Aptamer-Based Electrochemical Biosensor for Rapid Detection of Mercury (II) and Lead (II) Ions in Water

1
Material and Engineering Research Institute, Sheffield Hallam University, Sheffield S1 1WB, UK
2
Faculty of Science, University of Basrah, Basrah 61004, Iraq
3
Biomolecular Research Centre, Sheffield Hallam University, Sheffield S1 1WB, UK
*
Author to whom correspondence should be addressed.
Chemosensors 2019, 7(2), 27; https://doi.org/10.3390/chemosensors7020027
Received: 21 March 2019 / Revised: 7 May 2019 / Accepted: 28 May 2019 / Published: 4 June 2019
In this work, we report on the development of an electrochemical biosensor for high selectivity and rapid detection of Hg2+ and Pb2+ ions using DNA-based specific aptamer probes labeled with ferrocene (or methylene blue) and thiol groups at their 5′ and 3′ termini, respectively. Aptamers were immobilized onto the surface of screen-printed gold electrodes via the SH (thiol) groups, and then cyclic voltammetry and impedance spectra measurements were performed in buffer solutions with the addition of HgCl2 and PbCl2 salts at different concentrations. Changes in 3D conformation of aptamers, caused by binding their respective targets, e.g., Hg2+ and Pb2+ ions, were accompanied by an increase in the electron transfer between the redox label and the electrode. Accordingly, the presence of the above ions can be detected electrochemically. The detection of Hg2+ and Pb2+ ions in a wide range of concentrations as low as 0.1 ng/mL (or 0.1 ppb) was achieved. The study of the kinetics of aptamer/heavy metal ions binding gave the values of the affinity constants of approximately 9.10−7 mol, which proved the high specificity of the aptamers used. View Full-Text
Keywords: aptamer sensors; electrochemical sensors; heavy metal ions; cyclic voltammetry; impedance spectroscopy; binding kinetics aptamer sensors; electrochemical sensors; heavy metal ions; cyclic voltammetry; impedance spectroscopy; binding kinetics
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MDPI and ACS Style

Abu-Ali, H.; Nabok, A.; Smith, T.J. Development of Novel and Highly Specific ssDNA-Aptamer-Based Electrochemical Biosensor for Rapid Detection of Mercury (II) and Lead (II) Ions in Water. Chemosensors 2019, 7, 27. https://doi.org/10.3390/chemosensors7020027

AMA Style

Abu-Ali H, Nabok A, Smith TJ. Development of Novel and Highly Specific ssDNA-Aptamer-Based Electrochemical Biosensor for Rapid Detection of Mercury (II) and Lead (II) Ions in Water. Chemosensors. 2019; 7(2):27. https://doi.org/10.3390/chemosensors7020027

Chicago/Turabian Style

Abu-Ali, Hisham, Alexei Nabok, and Thomas J. Smith 2019. "Development of Novel and Highly Specific ssDNA-Aptamer-Based Electrochemical Biosensor for Rapid Detection of Mercury (II) and Lead (II) Ions in Water" Chemosensors 7, no. 2: 27. https://doi.org/10.3390/chemosensors7020027

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