Next Article in Journal
Recent Progress on the Evolution of Pourbaix Sensors: Molecular Logic Gates for Protons and Oxidants
Previous Article in Journal
Advancements and Potential Applications of Microfluidic Approaches—A Review
Previous Article in Special Issue
Modelling and Development of Electrical Aptasensors: A Short Review
Article Menu

Export Article

Open AccessCommunication
Chemosensors 2018, 6(4), 47; https://doi.org/10.3390/chemosensors6040047

IR-783 Labeling of a Peptide Receptor for ‘Turn-On’ Fluorescence Based Sensing

1
Department of Pharmacology, College of Medicine, University of Arizona, 1501 N. Campbell Ave., Tucson, AZ 85724, USA
2
Department of Bioengineering, University of Texas, Arlington, 500 UTA Blvd., Arlington, TX 76010, USA
*
Author to whom correspondence should be addressed.
Received: 22 August 2018 / Revised: 27 September 2018 / Accepted: 12 October 2018 / Published: 16 October 2018
(This article belongs to the Special Issue Aptamer Technologies)
  |  
PDF [1131 KB, uploaded 16 October 2018]
  |  

Abstract

In this study, we examine a means for developing near-IR fluorescent sensors through streamlined, site-specific coupling with peptide-based receptors. As the penultimate step of solid-phase synthesis of a peptide-based receptor, we show a simple means of labeling the N’ terminus with the near IR fluorophore IR-783 to afford a viable fluorescent sensor after cleavage from the resin. The proof-of-concept probe utilized a biotin mimetic peptide sequence as the receptive moiety. Here we revealed a “turn-on” fluorescence enhancement upon binding of the biotin mimetic probe to its intended streptavidin target. Not all peptide-receptive moieties tested were able to generate such an enhancement upon target binding, and as such, the rationale for the observed fluorescence response properties is discussed. View Full-Text
Keywords: peptide; probe; fluorescence; sensor peptide; probe; fluorescence; sensor
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Kwak, E.-A.; Kydd, L.; Lim, B.; Jaworski, J. IR-783 Labeling of a Peptide Receptor for ‘Turn-On’ Fluorescence Based Sensing. Chemosensors 2018, 6, 47.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Chemosensors EISSN 2227-9040 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top