Aptamer-Based Electrochemical Sensing of Lysozyme
1
International Center of Biodynamics, 1B Intrarea Portocalelor, Sector 6, Bucharest 060101, Romania
2
Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR CNRS 8520, Lille1 University, Avenue Poincaré-BP60069, Villeneuve d’Ascq 59652, France
3
Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials, Shandong University, Jinan 250061, China
*
Authors to whom correspondence should be addressed.
Academic Editors: Paolo Ugo and Ligia Moretto
Chemosensors 2016, 4(2), 10; https://doi.org/10.3390/chemosensors4020010
Received: 10 March 2016 / Revised: 28 May 2016 / Accepted: 8 June 2016 / Published: 14 June 2016
(This article belongs to the Special Issue Electrochemical Immunosensors and Aptasensors)
Abstract
Protein analysis and quantification are required daily by thousands of laboratories worldwide for activities ranging from protein characterization to clinical diagnostics. Multiple factors have to be considered when selecting the best detection and quantification assay, including the amount of protein available, its concentration, the presence of interfering molecules, as well as costs and rapidity. This is also the case for lysozyme, a 14.3-kDa protein ubiquitously present in many organisms, that has been identified with a variety of functions: antibacterial activity, a biomarker of several serious medical conditions, a potential allergen in foods or a model of amyloid-type protein aggregation. Since the design of the first lysozyme aptamer in 2001, lysozyme became one of the most intensively-investigated biological target analytes for the design of novel biosensing concepts, particularly with regards to electrochemical aptasensors. In this review, we discuss the state of the art of aptamer-based electrochemical sensing of lysozyme, with emphasis on sensing in serum and real samples. View Full-TextKeywords:
lysozyme; aptamers; surface modification; electrochemical methods
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Vasilescu, A.; Wang, Q.; Li, M.; Boukherroub, R.; Szunerits, S. Aptamer-Based Electrochemical Sensing of Lysozyme. Chemosensors 2016, 4, 10.
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