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Article

Comparison of Surface and Spectral Properties of Optical Sensor Layers Prepared by Spin/Spray Coating and Printing Techniques

by
Nena Dimitrušev
1,2,3,*,
Polonca Nedeljko
2,
A. F. P. Allwin Mabes Raj
2,4,5 and
Aleksandra Lobnik
2,3
1
Messer Slovenija, Jugova ulica 20, 2342 Ruše, Slovenia
2
Institute for Environmental Protection and Sensors, Beloruska 7, 2000 Maribor, Slovenia
3
Faculty of Mechanical Engineering, Centre of Sensor Technology, University of Maribor, Smetanova 17, 2000 Maribor, Slovenia
4
Department of Environmental Science, Jožef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia
5
Jožef Stefan International Postgraduate School, Jamova 39, 1000 Ljubljana, Slovenia
*
Author to whom correspondence should be addressed.
Chemosensors 2023, 11(2), 136; https://doi.org/10.3390/chemosensors11020136
Submission received: 3 November 2022 / Revised: 31 January 2023 / Accepted: 7 February 2023 / Published: 13 February 2023
(This article belongs to the Section Optical Chemical Sensors)

Abstract

:
This study investigated the surface properties of optical sensor layers prepared using sol-gel technology and their response to dissolved NH3. A glass substrate was used to fabricate the optical sensor layers. The sol-gel solution was applied to the glass substrate using three different techniques: spin coating (SC), inkjet printing (IP), and spray coating (SP). In this work, we have attempted to investigate the effects of the different techniques for producing the sensor layers and to determine their response in the presence of ammonia. The surface properties (surface free energy—SFE and surface chemical composition—XPS) and spectral properties (response to ammonia and real-time response) of the prepared optical sensor layers were characterised. The results show that the sensor layers prepared by different techniques have similar SFE and XPS values, but different responses to dissolved NH3 solution and different responses in real-time measurements (exposure to fresh fish). Sensor layers prepared with a spray coating (SP) are the most responsive, the most sensitive, and have a higher response over time and the biggest colour change compared to SC and IP sensor layers.

1. Introduction

Compared to conventional electrical sensors, the advantages of optical chemical sensors are insensitivity to electromagnetic interference, selectivity, and safety when working with flammable and explosive compounds. They are widely used because of their many capabilities, such as sensitivity, cost-effectiveness, and non-destructiveness. The sol-gel process offers a simple and easy way to obtain thin films with high purity, homogeneous composition, and good properties in just one step and at a low cost [1].
The basis to produce optical chemical sensors is usually an indicator dye, which is immobilized in the corresponding polymer base according to the chosen immobilization method. The properties of the sol-gel material and its final shape can be influenced by various process parameters. A major influence on the final properties of the optical sensor is the immobilization of the indicator in a suitable polymer carrier, which can be in the form of thin membranes—sensor layers, gels, optical fibers, parts, etc.-etc. Thin polymeric supports are most used to produce sensor layers. The sensing layers can be produced by various application techniques. However, the sensory properties of optical chemical sensors can also be altered by the technique of applying the sol-gel solution to a suitable substrate.
Optical chemical sensors can be found in various fields, such as medicine, biotechnology, personal protection, and the chemical industry. Recently also for medical applications such as disease diagnosis from breath analysis [2]. Optical chemical sensors can be manufactured by various methods, but in recent years the sol-gel method is one of the most used techniques. The sol-gel method allows the fabrication of optical sensors and biosensor layers for the measurement of pH, gas vapours and ionic species, as described by Jeronimo et al. [3]. Other reports over 10 years (2005–2015) deal with sol-gel-based optical sensor layers (absorption and luminescence) for analysts such as oxygen, pH, ammonia, carbon dioxide and heavy metal ions [1,4]. Optical pH sensor based on sol-gel was also investigated by Zhang et al. [5]. Different sensors from different research groups were developed in the environmental field: Biring et al. [6] develop dual gas sensors for the simultaneous detection of oxygen and ammonia gases, and optical hydrogen sensors were developed by a group of Matsuura et al. [7]. For the detection of hydrogen peroxide was developed ratiometric fluorescence sensors based on CgSe/ZnS quantum dots by Doung et al. [8].
In environmental protection, monitoring environmental parameters is crucial to control or limit excess concentrations that can be lethal to living organisms. One of these parameters is ammonia gas (NH3), which occurs naturally in the atmosphere and is also widely used in various industrial and agricultural activities. In most cases, ammonia is used in the production of fertilizers and other chemical substances. Existing and standardized methods for determining the concentrations of various environmental parameters are time-consuming and require specialized sample preparation and highly skilled personnel. Therefore, there is a need to develop alternative methods for the determination of environmental parameters with analytical devices that are small and portable and allow the acquisition of analytical signals on-site, continuously (online), and at remote locations. Optical chemical sensors are one such alternative.
The most used techniques for the preparation of sensors are spin coating, dip coating, inkjet printing, and, in recent years, most commonly, spray coating. Spin coating is currently the laboratory standard for the rapid development of small-area films from solution [9] and has been used to produce uniform coatings of desired thickness [10]. However, the spin-coating technique has a lot of disadvantages, such as large amounts of waste [9,10] and incompatibility with reel-to-reel or large-area processing [9,11,12]. It is not readily scalable [9]. The spin coating technique is also more sensitive to variations in precursor compositions, resulting in sharper changes in the size of surface features [13]. The biggest problem of the spin coating technique is problematic manufacture in a large area, while inkjet printing and spray coating are techniques that can fulfil all requirements for large-scale production [14].
Inkjet printing is an important tool in industrial mass fabrication and one of the most versatile methods available for prototyping [15]. It is used in research laboratories for the fabrication of (bio)chemical sensing devices or single functional elements of such devices [16]. With inkjet printing, electronic data can be transferred to paper or overhead transparencies. It is one of the key technologies in the field of defined polymer deposition [17]. More recently, inkjet technology used in the fields of electronics and mechanics has been adopted for the deposition of biological reagents onto surfaces. This kind of printing involves the digitally controlled ejection of drops of fluid from a print head onto a substrate [18]. The inkjet printing system is simple, rapid [15,19,20], low cost, high resolution, flexible, and non-contact, and generates a low amount of waste [15,16,20,21]. It also allows room temperature deposition, applies to large areas and flexible substrates [20], and all the controlled deposition of small droplets of liquids (ink) onto a substrate [16,21]. The system is compatible with paper substrates and amenable to pattern formation [19]. The disadvantages of this system are that the short gelation time of silica sols can cause gelation and clogging of the inkjet nozzles [19], cavitation bubbles, undesired satellite drops, nozzle clogging, droplets instabilities, agglomeration, precipitation, or deposition of solutes [21]. Printability problems may occur when the particle size becomes of the order of 1 µm [17]. Rheological properties, such as viscosity and surface tension, are the most critical part of the formulation of the bio-ink [18,19]. A low ink viscosity can cause uneven jetting, but if the ink is too viscous, jetting will not occur [18]. The surface tension is responsible for the spheroidal shape of the liquid drop emerging from the nozzle [17,18].
Because of the disadvantages of these two techniques, the use of Spray coating for large-scale production of materials with different coatings is increased. Spray coating has many advantages compared with other techniques, namely: the ability to coat complex geometries at an industrial scale [1,22] and the ability to coat large surfaces with different morphologies and roughness [1]. During the deposition of the material, the amount discarded is minimized [11], and that’s what makes these techniques promising to substitute the spin coating technique [10]. With the spray coating technique, we can generate very thin layers on different substrates, which is promising for the industrial application of organic-inorganic thin films [22]. It is also very promising for large-area deposition with controlled thickness [9,10,11]. Thicknesses ranging from 250 to 450 nm can be produced with a single-pass spray process [11] and it is not directly proportional to the number of layers but to the deposited mass [23]. Using spray coating, different thicknesses can be achieved by changing different parameters, but the most important are pressure [1,22] and the distance between the extreme of the gun and the target [1,10,22]. How deposition occurs depends also on the speed of the gun in the lateral movement (x-axis), the distance between depositions in the y-axis [22], the fluid viscosity, and the shape and size of the nozzle (size is usually fixed by the spray gun) [1]. The amount that can be applied to the surface depends on the nozzle speed (s), flow rate (FR) (i.e., rate of liquid ejection from the spray nozzle), and nozzle height (h) (distance from the substrate) [24]. The latter (distance from the substrate) has a great impact on the morphology of the deposited layer [10]. By changing parameters such as distance and speed of depositions, roughness can be changed. At larger distances and higher speeds, the surface roughness decreases [1].
In Table 1, we list the sensors for NH3 (gas or aqueous) prepared with different coating techniques (spin coating, spray coating etc.).
Polyaniline-based ammonia gas sensors were investigated [14]. The authors prepared ammonia gas sensors with spray coating and inkjet printing and concluded that the different roughness of the layers made with inkjet printing did not contribute to a change in sensor performance.
Although measuring the contact angle and hydrophilicity/hydrophobicity of sol-gel films is critical for many sensing applications [4], there is very little, almost no, research on how surface properties affect the responsiveness of optical sensing layers and how different sensor fabrication techniques affect responsiveness.
The novelty of this study is the comparison of the surface properties of sensor layers prepared by three different deposition techniques: spin coating (SC), inkjet printing (IP) and spray coating (SP) and the simultaneous monitoring of the effects of different deposition techniques on the response of the sensor in the presence of dissolved ammonia (NH3). This work may be useful for a deeper understanding of the fact that different preparation techniques of sensor layers have different effects on their response in the presence of the analyte.

2. Materials and Methods

2.1. Preparation of Optical Sensor Layers

In this study, a glass substrate (d = 20.0 mm, thickness = 1.6 mm) was used as a solid support onto which the sol-gel solution was applied. The glass substrate was activated by treatment with concentrated nitric acid (65%; HNO3) for 24 h, then washed with distilled water and ethanol and dried at 70 °C for 24 h. Afterwards, the glass substrate was prepared for sol-gel application using various techniques. The sensor solutions were prepared by combinations of hydrophilic and hydrophobic sol-gel precursors and absorption indicator dyes. The sol-gel process was carried out using an acid-catalysed method. Detailed information on the composition of the sol-gel solutions is confidential.
Sensor layers were prepared with three different techniques: spin coating, inkjet printing and spray coating.

2.1.1. Spin Coating

Sensor thin layers were prepared using a WS-650MZ-23NPP/LITE spin coater from Laurell Technologies Co. (Lansdale, PA, USA). A 100 μL aliquot of the solution was spread on a glass substrate and spin-coated at a maximum of 1500 rpm for 30 s. The prepared optical sensor layers were marked as Sensor_SC.

2.1.2. Inkjet Printing

Inkjet printing was carried out using a Dimatix inkjet printer (DMP-2831) with DMP-2800 series software (Fujifilm Dimatix Inc., Santa Clara, CA, USA). For printing, a 10 pL cartridge was used and optical sensor layers were printed using 50 µm of Drop spacing. The prepared optical sensor layers were marked as Sensor_IP.

2.1.3. Inkjet Printing

For spray coating was used an automatic self-made system equipped with an Airbrush spray gun and Inkscape vector graphics software. Optical sensor layers were prepared with one sprayed layer and 1 bar of pressure with a 10 cm distance between the gun and glass substrate. The prepared optical sensor layers were marked as Sensor_SP.

2.2. Characterization of Optical Sensors

In this study, the surface properties of the prepared optical sensor layers with different techniques were studied. The spectral properties were also studied through the determination of the responsiveness of the optical sensors in an aqueous medium, and the real-time response with the determination of the colour of tested optical sensors on the responsiveness to fresh fish.
A schematic representation of the response of the optical sensors to NH3 is presented in Figure 1.

2.2.1. ATR-FTIR Spectroscopy

ATR-FTIR were recorded on a Perkin Elmer spectrometer. The absorbance measurements were carried out within the range of 450–4000 cm−1 with 16 scans and a resolution of 4 cm−1.

2.2.2. Surface Free Energy (SFE)

The surface free energy was calculated with contact angle (CA) measurements and surface-free energy (SFE) calculations. For the CA measurements, an OCA5 contact-angle measurement system from Dataphysics (Filderstadt, Germany) was used. CA was determined from the tangent of the sessile drop profile at the three-phase contact point drown onto the photoprint. This method was used for the surface free energy calculation, using three different liquids: Milli-Q water, diiodomethane, and formamide. The total SFE (ɣsTOT) was calculated according to [33] and the SFE components were then calculated according to [34]. Total surface free energy is divided into Lifshitz–van der Waals interactions (ɣsLW) and acid-based interactions (ɣsAB). Lifshitz–van der Waals interactions comprise dispersion, dipolar, and induction interactions, while acid-based interactions comprise all the electron donor-acceptor interactions, like hydrogen bonding. The acid-base interactions are also subdivided into electron donor ɣs (Lewis base) and electron acceptor ɣs+ (Lewis acid) parts [34].

2.2.3. Surface Chemical Composition (XPS)

Surface chemical composition with determining amounts of differently bound carbons was investigated with X-ray photoelectron spectroscopy (XPS) using a PHI-TFA XPS spectrometer produced by Physical Electronics Inc. (Chanhassen, MN, USA). For the analysis, a sensor area of 0.4 mm in diameter was used. A pass energy of 29 eV (using an energy analyzer operating at a resolution of 0.6 eV) was used to acquire the high-energy resolution spectra, with an accuracy of the binding energy of about 0.3 eV. Surface composition was quantified from XPS peak intensities considering relative sensitivity factors provided by the instrument manufacturer [35].

2.2.4. Avantes AvaSpec Spectrophotometer

The spectral properties (absorbance measurements) of the optical sensor layers in the presence of ammonia were performed with an Avantes AvaSpec spectrophotometer. The spectrophotometer was connected to a flow cell that has been custom designed. An optical chemical sensor was placed in the flow cell and the sample solution was let into the cell. Using a peristaltic pump (Heidolph Pumpdrive 5001) the flow rate of the sample solution was kept constant and was 7.5T mL/min. The spectrophotometer used an AvaLight DHC-light source. The detector was set to 10 ms integration time with 40 cycles and the number of points for smoothing the spectral data was set to 5. For generating the analytical signal Avasoft 7.6 software was used.
With the system, the Avantes AvaSpec spectrometer also determined the response time (Time drive) of prepared sensor layers in buffer pH7 and a certain concentration of ammonia (5 × 10−3 M NH4Cl). To this end, 5 × 10−3 M NH4Cl was dissolved in pH7 buffer from a stock 0.1 M solution of ammonium chloride in pH7 buffer. We started with the introduction of the pH buffer and after 10 min, the signal was stable. Then, we alternately added 5 × 10−3 M NH4Cl and buffer pH7 for 30 min each, two times.

2.2.5. Real-Time Response of Optical Sensors

The real-time response of the prepared optical sensor layers was determined using EOPTIS CLM19x colourimeter analysis. Optical sensor layers were exposed to fresh fish (from the market) in a closed container under real conditions in the refrigerator. The colour change of the sensor layers in the container in the presence of fish was measured daily.

3. Results

3.1. Preparation of Optical Sensor Layers with Different Techniques

In this article, we present three different techniques to produce optical sensor layers: spin coating, inkjet printing, and spray coating. All techniques are suitable to produce optical sensor layers. The optical sensor layers produced using the three different techniques are shown in Figure 2.
It can be seen from Figure 2 that all sensor layers have a light-yellow colour and a thin layer. In the case of the optical sensor layers produced by coating (spin coating and spray coating), the entire glass surface is covered with the solution, while in the case of the optical sensors produced with the inkjet printing process, only the centre is covered in the form of a rectangle that was used for printing. The sensor layers produced with the spin coating (Figure 2a) are slightly yellow in the middle and more intensely yellow at the edges. The coating also has a very flat surface. The sensor layer produced with inkjet printing (Figure 2b) has a rectangle shape and the entire rectangle is covered with solution. These optical sensor layers are thicker and rougher and have a distinct roughness. The sensor layer produced with spray coating (Figure 2c) has a flat surface that is completely covered with the solution.

3.2. Characterization of Optical Sensor Layers

3.2.1. ATR-FTIR Spectroscopy

ATR-FTIR spectroscopy was used to confirm the efficiency of the preparation of optical sensor layers on glass carriers. The results are represented in Figure 3. Only the region from 2000 cm−1 to 500 cm−1 is shown.
Figure 3 shows the FTIR spectra of optical sensor layers prepared on a glass substrate using the three different techniques. The spectra of the differently prepared sensor layers are represented by different coloured lines: without application of sol-gel solution (pure glass—black line), spin coating (SC—red line), inkjet printing (IP—blue line), and spray coating (SP—green line).
Two peaks are visible on the spectrum of the pure glass substrate (black line). The first peak is at 916 cm−1, characteristic of Si-O-Si (oxygen-silicon bond) and the second peak is at 767 cm−1, characteristic of Si-O vibrations [36]. In the other three cases, where the sensor layers were prepared using different application techniques, identical peaks were present. The only difference is in the intensity of the peaks, which is the least intense in the case of the sensor coating produced with the inkjet printing technique (blue line). In all four spectra, the same peaks are seen in the range from 1250 cm−1 to 700 cm−1 characteristic of silica [37], while the range around 1430 cm−1 to 1625 cm−1 is characteristic of C-C stretches in the region [38]. The peak at around 1600 cm−1 is characteristic of C-C in the ring [39]. The peak at around 1430 cm−1 is characteristic of the stretching of silicon attached to the ring (Si-C6H5) [39,40]; the peak at 1130 cm−1 is characteristic of Si-O-Si asymmetric stretching [40] or Si-O-CH3 [39] and the peak at around 1050 cm−1 is characteristic of Si-O-C stretching [40]. The peaks at 736 cm−1 and 696 cm−1 are characteristic of ring breathing [37], which is presented in ormosil and used for preparing optical sensor layers.
ATR-FTIR spectroscopy confirmed that the glass substrate was successfully covered with solution (for preparation of optical sensor layers) with the presentation of the same functional groups on all optical sensor layers.

3.2.2. Water Contact Angle and Surface Free Energy

Hydrophilic/Hydrophobic Character of Glass and Optical Sensor Layers Prepared with Different Techniques

The hydrophilic/hydrophobic character is calculated by the water contact angles (WCA) measurements. The results for the uncoated glass and glass substrates with optical sensor layers prepared by the three different techniques are shown in Table 2 and are the average of at least five measurements.
From Table 2, it can be seen that the uncoated glass substrate has a very small water contact angle of 21.19 ± 0.63°. This indicates a hydrophilic character of the optical sensor layers, because of the presence of silanol groups (Si-O-H) on the surface of glass [41], also proved by FTIR analysis (Figure 3).
The optical sensor layers made with spin coating and spray coating have almost the same hydrophilic/hydrophobic character, with a contact angle of 80°, while the optical sensor layers made with inkjet printing have a higher contact angle of around 84°. This is probably due to the higher roughness of the surfaces of the optical sensor layers made by inkjet printing. Films made by Inkjet printing have high visible roughness. The roughness was so large it was impossible to measure with the AFM technique. Kangur et al., reported that high contact angle and high surface roughness are more or less correlated [42]. The reason for the higher contact angle with water in the case of the optical sensor layers is also a result of nonpolar groups presented in the ormosils used for making sensors (Figure 3).

Surface Free Energy of Glass and Optical Sensor Layers Prepared with DifferentTechniques

The results of surface free energy (SFE) calculations of the substrate and optical sensor layers prepared with different techniques are represented in Figure 4. There are represented total surface free energy (ɣsTOT) and Lifshitz–van der Waals contributions (ɣLW), which represent the nonpolar part and the polar part, which is divided into the electron-donor contribution (ɣ−) and the electron-acceptor contribution (ɣ+).
All these parameters are calculated from contact angle measurements with three test liquids performed using goniometry. These liquids are water, formamide, and diiodomethane.
The SFE (ɣsTOT) of the glass substrate is the highest at 53.99 ± 1.63 mJ/m2, with the highest electron donor (ɣ−) component (52.36 ± 1.02 mJ/m2). This is considered a high-energy surface.
The surface free energies of the optical sensor layers prepared with spin coating and spray coating are the same, with an SFE (ɣsTOT) of around 29 mJ/m2, which is smaller by around 45% in comparison with the glass substrate. The electron donor component (ɣ−) is around 10 mJ/m2 and is smaller by around 80% in comparison with the glass substrate. Lifshitz–van der Waals contributions (ɣLW) are around 30 mJ/m2 and are increased by around 2.6% for optical sensor layers made by spin coating and 5.2% for optical sensor layers made by spray coating.
From all SFE results, optical sensor layers made with inkjet printing have the highest SFE (ɣsTOT), at around 34 mJ/m2, and the lowest electron donor component, around 4 mJ/m2. The total SFE (ɣsTOT) is smaller by around 37% and the electron donor component is smaller by around 92% in comparison with the glass substrate.

3.2.3. X-ray Photoelectron Spectroscopy (XPS)

X-ray photoelectron spectroscopy was used to determine the surface elemental composition and relative amounts of differently bound carbons in the glass and optical sensor layers prepared with different techniques: spin coating, inkjet printing, and spray coating. The results are represented in Table 3 and Table 4.
Different elements presented on the surface of the glass are characteristic of the substrate material. The O/C ratio of glass is 0.73, which means that glass is hydrophilic, which is proved by the measurements of the hydrophilic/hydrophobic character and the polar component of the surface free energy (Table 2 and Figure 4).
Optical sensor layers made with spin coating and spray coating have almost identical surface elemental composition, while optical sensor layers made by inkjet printing are a little different but still broadly the same. All optical sensor layers contain about 62–64 at. % of C, 22–24 at.% of O and 12–14 at.% of Si. The O/C ratio is also the same. In all cases, the amount of C is increased by more than 30% and the content of O decreased for almost the same amount. The Si content also decreased by around 7% in the case of spin coating and inkjet printing and by around 16% in the case of spray coating. The O/C ratio also decreased to around 47% in the case of optical sensor layers prepared with inkjet printing and to around 52% in the case of optical sensor layers prepared with coating techniques. The O/C ratio indicates that the surfaces of all optical sensor layers are more hydrophobic, which is proved by the determination of SFE (Figure 4).
From Table 4 we can see that relative surface carbon amounts involved in the C-C bonds in the case of optical sensor layers are higher compared to the glass substrate, and the C-O, C-OH, and C-Si carbon fractions also increased. The glass substrate contains carboxylic O-C=O groups. The presence of carboxylic groups is responsible for the hydrophilic character of the glass substrate (Table 2) and the highest O/C ratio (Table 3).
From these results, we can also see that using different techniques for preparing optical sensor layers did not affect the differently bonded carbon atoms. All optical sensor layers have similar surface chemical characteristics. In all cases, C atoms are bonded similarly: C-C and C-H bonds represent around 90% of C-atoms and 6–10% are bonded in C-O/C-OH/C-Si bonds.
Optical sensor layers prepared with coating techniques (spin and spray) have higher carbon amounts involved in the C-C bond by around 3%, meanwhile optical sensor layers prepared with inkjet printing the figure is 5%. Carbon fractions of C-O, C-OH, and C-Si are for optical sensor layers prepared with coating techniques higher by around 30% and for optical sensor layers prepared with inkjet printing smaller by around 1%.
These results are in good correlation with the SFE results (Figure 4). The portions of carbon involved within the C-C bonds on the surface of the glass substrate is 88.5% and on the surface of optical sensor layers prepared with different techniques is more than 91%. This means that optical sensor layers have a bigger amount of C-C bonds compared to a glass substrate by 2.8% and 3% in the case of optical sensor layers prepared with spin coating and spray coating respectively and by around 5% in the case of optical sensor layers prepared with inkjet printing. At the same time, the Lifchitz–van der Waals contribution (γsLW) of the surface free energy is bigger by 2.6% in the case of optical sensor layers prepared with spin coating, 5.2% in the case of optical sensor layers prepared with spray coating and 7.4% in the case of optical sensor layers prepared with inkjet printing. The glass substrate has carbon fractions involved within the C-OH and O=C-O bonds of about 6.9 and 4.6%, respectively. The content of these polar groups proves that the glass substrate has a hydrophilic character. This is also proved by the O/C ratio, which is 0.73. Optical sensor layers prepared with different techniques had no O=C-O groups, but optical sensor layers prepared with coating techniques have a bigger amount of C-OH and C-Si groups by around 30%. Optical sensor layers prepared with inkjet printing have almost the same amount of C-OH and C-Si groups as the glass substrate. This is proof of the smaller amount of polar distribution of all the optical sensor layers and the hydrophobic character of all the optical sensor layers. All the polar contribution in the case of the optical sensor layers is because of the presence of C-OH groups, which are present in a small amount. Electron donor contributions are smaller by around 80% in the case of optical sensor layers prepared with coating techniques and by more than 90% in the case of optical sensor layers prepared with inkjet printing. The O/C ratio of all the optical sensor layers is smaller by 52.1% in the case of optical sensor layers prepared with coating techniques and by 47% in the case of optical sensor layers prepared with inkjet printing. Meanwhile, the total SFE of optical sensor layers also decreased by around 45% and 37% in the case of optical sensor layers prepared with coating techniques and inkjet printing, respectively.
If we compare all the optical sensor layers, we can conclude that optical sensor layers prepared with coating techniques have almost identical surfaces with the same total SFE, the same Lifchitz–van der Waals contribution and the same electron donor and electron acceptor contribution. Optical sensor layers prepared with inkjet printing have higher total SFE, higher Lifchitz–van der Waals contribution and smaller electron donor and electron acceptor contribution compared to optical sensor layers prepared with coating techniques. Optical sensor layers prepared with inkjet printing also have the highest O/C ratio and the highest amount of C-C bonds and the smallest amount of C-OH and C-Si groups.

3.2.4. Spectral Properties of Sensor Layers in the Presence of Ammonia

Response to Ammonia in an Aqueous Medium

The response to ammonia was determined through absorbance measurements of optical sensor layers in different concentrations of ammonia. The results are represented in Figure 5 and Figure 6.
Figure 5 presents the absorption spectra of the sensor layers prepared by the three different techniques; Figure 5a spin coating (SC), Figure 5b inkjet printing (IP), and Figure 5c spray coating (SP) in the presence of dissolved NH3 in the concentration range between 1.0 × 10−5 M and 7.0 × 10−1 M.
The sensor layers (SC, IP, SP) showed similar spectral properties in the presence of dissolved NH3 (1.0 × 10−5 M–7.0 × 10−1 M). The intensity of the absorption peak at 600 nm (blue colour) increased in the presence of higher NH3 concentration, whereas the absorption peak between 400 and 460 nm (yellow colour) decreased. In the case of the sensor layer SP, the increase in the absorption peak at 600 nm is the highest, while in the case of IP, the increase is the smallest. Inkjet printing takes place at an elevated temperature and the sol-gel is probably more cross-linked and the dye less accessible, as a result, which is reflected in the response of the sensor coating, which in this case is the lowest. The difference between spray and spin coating is due to the physically more accessible indicator in the case of spray coating. In spin coating, a lot of the sol-gel solution is dispersed during the spin itself, which results in less dye on the surface of the glass substrates. Unlike with spin coating, when spray coating is used, the solution is concentrated on a single point of the surface, which results in better accessibility of the dye, as is clearly visible in Figure 5c and Figure 6.
For a better comparison of the most optimal sensor application for NH3 detection, the spectral properties of the sensor layers (SC, IP, SP) are shown in Figure 6 as a function of different NH3 concentrations at constant wavelength, which also shows the working range of NH3 detection. The working range for the detection of dissolved NH3 with the individual types of sensor layers (SC, IP, SP) is shown as a change in the absorbance signal at maximum absorbance (Amax = 600 nm) as a function of the logarithmic values of the NH3 concentrations. We can determine in which concentration range the sensor layers (SC, IP, SP) are most sensitive to the NH3 or in which concentration range the signal change is directly proportional to the concentration of the dissolved NH3.
A comparison of the calibration curves showing absorbance as a function of logarithmic NH3 concentration was performed between sensor layers produced by the three different techniques. As can be seen in Figure 6, the highest signal change of 0.5 in the presence of NH3 is obtained with the sensor layer SP, while the lowest signal change of 0.16 occurred with the sensor layer IP. The sensor layer SC has a signal change of 0.3. For the sensor layer SP produced by spray coating, the signal change was 213% higher than the sensor layer IP produced by inkjet printing and 67% higher than the sensor layer SC produced by spin coating.
At lower concentrations of dissolved NH3, the optical SC and SP sensor layers have the same responsiveness. With increasing dissolved ammonia concentrations, responsiveness is higher for the optical sensor prepared with SP than for the optical sensor prepared with SC. The highest response to dissolved NH3 is by the optical sensor layers prepared by spray coating (10−5 M–3 × 10−1 M). Optical sensor layers prepared with inkjet printing (IP) are much less sensitive, especially in the lower concentration range of dissolved NH3.
If we compare the results in terms of manufacturing techniques, we can see that, in all three cases, optical sensor layers prepared with the three different techniques are responsive in the presence of low concentrations of dissolved NH3 but deviations between them occur at higher concentrations. At higher concentrations, sensor layers prepared with spray coating (SP) techniques are more responsive and sensitive than the sensor layers prepared with the other two techniques (SC, IP).

Response Time of Sensor Layers (Time Drive)

The response time, t95, of sensor layers prepared with different techniques was tested by continuous monitoring of absorbance changes at Amax 600 nm after the addition of NH4Cl (5 × 10−3 M) in buffer pH7 solution. The results are represented in Figure 7.
Figure 7 shows the response time of three different sensor layers in the absence and presence of NH4Cl (5 × 10−3 M). After exposure to the buffer solution signal was constant, when the sensor layer was exposed to the NH4Cl solution, the signal increased very steeply to an absorbance of 0.035 in the case of SP sensor layers and an absorbance of 0.01 in the case of the sensor layers SC and IP. After 30 min of exposure to the NH4Cl solution, the signal of the SP sensor layers had still not stabilized (with longer exposure the signal continued to increase). The SC and IP sensor layer signals stabilized after less than 10 min of exposure to the NH4Cl solution. When the buffer solution was reintroduced, the absorbance signal dropped almost to the original value in all three cases (SC, IP, SP). After the re-introduction of the NH4Cl solution (a further 30 min), the absorbance signal increased again to an even higher value than at the initial introduction of the solution. In the case of the SP layers, the absorbance value reached a value of 0.045, while in the case of SC and IP, the absorbance values were similar at 0.01. After the last cycle of introducing the buffer solution, the absorbance signal returned to almost the original value.
The results obtained from response time measurement are comparable to the results from the response to dissolved NH3 in solution. With this measurement, we also proved that the optical sensor layers prepared with the SP technique have a higher response compared to sensors prepared with the other two techniques (SC, IP). The sensor layers SC and IP have a much lower response.

3.2.5. Real-Time Response of Optical Sensor Layers

The real-time response of the prepared optical sensor layers (SC, IP, SP) was evaluated using EOPTIS CLM19x colourimeter analysis based on the colour change. Optical sensor layers were exposed to fresh fish (from the market) in a closed container under real conditions in the refrigerator over time (6 days). The colour change in the sensor layers was monitored and measured daily. The colour change in the sensor layers is the result of a reaction between the indicator inside the sensor layers and the analyte, which is released during the ageing of the fish meat. When the fish is fresh, the colour of the sensor layers is yellow and when the fish is spoiled, the colour changes from yellow to blue. The results of the colourimetric analysis of the different optical sensor layers are summarized in Table 5 and graphically shown in Figure 8.
From Table 5, we can see that all three sensor coatings (SC, IP, SP) in the presence of analyte vapour show a colour change over time. The best response in terms of the largest colour change is shown by the SP sensor layers, which can also be seen in Figure 8. In these cases, the colour changed from yellow to green, and, finally, to blue, which indicates spoiled fish with a very bad smell. In the case of the SC optical sensor layers, the colour changes from yellow to green. On the last day, when the fish was spoiled, the sensor layers were still green. The IP sensor layers prepared with the inkjet printing technique were brighter compared to the other two techniques (SC, SP). In this case, the colour change went from yellow to light green.
To summarize, sensor layers prepared with the spray coating (SP) technique give the best results in terms of maximum colour change in the presence of analyte vapour released during spoilage of fish meat. When the fish was spoiled, the SP sensor layers were clearly blue, while the IP and SC sensor layers were still green.
Figure 8 is a graphical representation of the measurement results from Table 5. We can see that the smallest colour change was determined in the case of optical sensor layers prepared by inkjet printing (IP), a larger colour change was determined in the case of optical sensor layers prepared by spin coating (SC), and the biggest colour change was determined in the case of optical sensor layers prepared by spray coating (SP). These results are very comparable to those obtained in an aqueous medium represented by the calibration curves in Figure 6. In all cases, the sensor receptor prepared with the spraying technique (SP) shows a better response in the presence of the analyte.

4. Conclusions

In this study, three different preparation methods (spin coating, inkjet printing and spray coating) of optical sensor layers were used to verify surface properties and responsiveness to ammonia. ATR-FTIR spectroscopy confirmed that the glass substrate was successfully covered with a solution with the presentation of the same functional groups on all optical sensor layers. The water contact angle (WCA) and surface free energy are the same for all the optical sensor layers, especially for optical sensor layers made with spin and spray coating. The WCA for the SC and SP optical sensor layers is around 80°, and for IP optical sensor layers is 83°. The surface free energy is also the same for the SC and SP optical sensor layers at 29 mJ/m2, while for the IP optical sensor layer, it is 34 mJ/m2. All the optical sensor layers have the same surface elemental composition (with an atomic proportion of C of around 63 at. %, O of 23 at. %, and Si of 14 at. %) and the same amounts of differently bound carbons (C1 = 91%; C2 = 9%, and without C4).
Although all the surface properties are the same, all the optical sensor layers (SC, IP, SP) have different responses to dissolved NH3 solution and different responses in real-time measurements (exposure to fresh fish). If we compare results from the response of the optical sensor layers (SC, IP, SP) in the presence of dissolved NH3 in an aqueous solution according to the manufacturing technique, we can see that all the optical sensor layers are responsive at low concentrations. At higher concentrations, the SP sensor layers are more responsive and sensitive than the SC and IP sensor layers, which are, at higher concentrations, much less sensitive.
A similar response of the three different sensor layers (SC, SP, IP) was also confirmed by time-drive measurements in the presence of NH4Cl solution in buffer media and by colourimetric analysis of sensor layers in the presence of a real sample, fish meat. The higher response over time and the biggest colour change were found with the SP sensor layers. Only the SP optical sensor layers changed colour from yellow, through green, to blue when the fish was spoiled. In the case of the other two layers (SC, IP), blue was not reached. We can conclude that the spray coating technique is the best for the optical sensor layers preparation for amine compound determination applications.

Author Contributions

Conceptualization, N.D., P.N. and A.F.P.A.M.R.; Methodology, N.D. and A.L.; Formal analysis, N.D. and P.N.; Investigation, N.D. and A.F.P.A.M.R.; Supervision, A.L.; Project administration, A.L. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the Ministry of Education, Science and Sport, Republic of Slovenia and European Union, European Regional Development Fund (ERDF), Early Research Careers 2.1 (Contract No. C3330-19-952032). The research work was also conducted within the framework of the Research Program P2-0438 Optical chemical/biosensor systems (OPTISENS). This work is also supported by an international research project, the Marie Skłodowska-Curie Action Global Mercury Observation and Training Network in Support to the Minamata Convention, financed under the funding line “excellent science” of the Horizon 2020 research and innovation program of the European Commission under the Marie Skłodowska-Curie grant agreement no. 860497.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Data available on request due to commercial restrictions.

Acknowledgments

We also want to thank Miran Mozetič and Janez Kovač from the Institute Jožef Stefan for the XPS measurements and their interpretation.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. Schematic representation of the response of the optical sensors to NH3.
Figure 1. Schematic representation of the response of the optical sensors to NH3.
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Figure 2. Optical sensor layers produced on glass substrates using three different techniques: (a) spin coating (SC), (b) inkjet printing (IC), and (c) spray coating (SP).
Figure 2. Optical sensor layers produced on glass substrates using three different techniques: (a) spin coating (SC), (b) inkjet printing (IC), and (c) spray coating (SP).
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Figure 3. FTIR spectra of pure glass substrates without application of the sol-gel solution (pure glass—black line) and sol-gel coated glass substrates: spin coating (SC—red line), inkjet printing (IP—blue line), and spray coating (SP—green line).
Figure 3. FTIR spectra of pure glass substrates without application of the sol-gel solution (pure glass—black line) and sol-gel coated glass substrates: spin coating (SC—red line), inkjet printing (IP—blue line), and spray coating (SP—green line).
Chemosensors 11 00136 g003
Figure 4. Surface free energy of glass and optical sensor layers prepared with different techniques: spin coating (Sensor_SC), inkjet printing (Sensor_IP), and spray coating (Sensor_SP).
Figure 4. Surface free energy of glass and optical sensor layers prepared with different techniques: spin coating (Sensor_SC), inkjet printing (Sensor_IP), and spray coating (Sensor_SP).
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Figure 5. Spectral properties of sensor layers (SC, IP, SP) in the presence of dissolved NH3. (a) Glass_SC, (b) Glass_IP, (c) Glass_SP.
Figure 5. Spectral properties of sensor layers (SC, IP, SP) in the presence of dissolved NH3. (a) Glass_SC, (b) Glass_IP, (c) Glass_SP.
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Figure 6. Calibration curves for optical sensor layers (SC, IP, SP) in the presence of dissolved NH3 in the concentration range 1.0 × 10−5 M–7.0 × 10−1 M.
Figure 6. Calibration curves for optical sensor layers (SC, IP, SP) in the presence of dissolved NH3 in the concentration range 1.0 × 10−5 M–7.0 × 10−1 M.
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Figure 7. Response time of sensor layers (SC, IP, SP) in the presence of NH4Cl (5 × 10−3 M), pH 7.
Figure 7. Response time of sensor layers (SC, IP, SP) in the presence of NH4Cl (5 × 10−3 M), pH 7.
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Figure 8. Colour change of three different sensor layers (SC, IP, SP) in the presence of fish meat over time.
Figure 8. Colour change of three different sensor layers (SC, IP, SP) in the presence of fish meat over time.
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Table 1. Comparison of sensing characteristics of various sensors for NH3 detection in different media, prepared with coating techniques.
Table 1. Comparison of sensing characteristics of various sensors for NH3 detection in different media, prepared with coating techniques.
MaterialMethodMediaLOD (ppm)Response Time (s)Recovery Time (s)Ref.
3% Er-doped ZnONebulizer spraygas12512010[25]
CA matrixSpin coatinggas/13203600[6]
Sol-gelcoatingaqueous0.2300/[26]
WO3/Si; WO3/PSspin coatinggas/16.1; 14.119.7; 19.2[27]
1D supramolecular nanofibresdrop-castinghumidity<1 ppb2.052.25[28]
Pani-SnO2
Nanocomposite
Spin coatinggas/12–1580[29]
Sol-gelSpin coatinggas34<600480[30]
α-Fe O2 3-ZnOsol-gelSpin coatinggas/2020[31]
ZnOSpray pyrolysisgas/2025[32]
Sol-gelSpray coatingaqueous0.151500Present work
Table 2. Water contact angles (WCA) of substrates and optical sensor layers prepared with different techniques: spin coating (Sensor_SC), inkjet printing (Sensor_IP), and spray coating (Sensor_SP).
Table 2. Water contact angles (WCA) of substrates and optical sensor layers prepared with different techniques: spin coating (Sensor_SC), inkjet printing (Sensor_IP), and spray coating (Sensor_SP).
SamplesWater Contact Angles (˚)
Glass21.19 ± 0.63
Sensor_SC79.84 ± 1.41
Sensor_IP83.69 ± 2.46
Sensor_SP80.06 ± 1.36
Table 3. Surface elemental composition of the substrate (glass) and optical sensor layers prepared with different techniques: spin coating (Sensor_SC), inkjet printing (Sensor_IP), and spray coating (Sensor_SP).
Table 3. Surface elemental composition of the substrate (glass) and optical sensor layers prepared with different techniques: spin coating (Sensor_SC), inkjet printing (Sensor_IP), and spray coating (Sensor_SP).
SamplesAtomic Proportion (at. %) of Surface Composition
COSiO/C
Glass47.434.815.20.73
Sensor_SC63.522.414.10.35
Sensor_IP61.624.114.20.39
Sensor_SP64.622.512.80.35
Table 4. Relative amounts of differently bound carbons obtained from the XPS survey spectra for the glass and optical sensor layers prepared with different techniques: spin coating (Sensor_SC), inkjet printing (Sensor_IP), and spray coating (Sensor_SP).
Table 4. Relative amounts of differently bound carbons obtained from the XPS survey spectra for the glass and optical sensor layers prepared with different techniques: spin coating (Sensor_SC), inkjet printing (Sensor_IP), and spray coating (Sensor_SP).
SamplesC1 (%)
C-C; C-H
C2 (%)
C-O; C-OH; C-Si
C4 (%)
O-C=O
Glass88.56.94.6
Sensor_SC91.09.00
Sensor_IP93.26.80
Sensor_SP91.28.80
Table 5. Colour changes (dEab*) of sensor layers (SC, IP, SP) in the presence of fish meat over time.
Table 5. Colour changes (dEab*) of sensor layers (SC, IP, SP) in the presence of fish meat over time.
SC_SensorIP_SensorSP_Sensor
dEab*ColourdEab*ColourdEab*Colour
Day 01.13Chemosensors 11 00136 i0011.19Chemosensors 11 00136 i0771.46Chemosensors 11 00136 i014
Day 11.69Chemosensors 11 00136 i0021.53Chemosensors 11 00136 i0087.7Chemosensors 11 00136 i015
Day 21.93Chemosensors 11 00136 i0032.03Chemosensors 11 00136 i0098.12Chemosensors 11 00136 i016
Day 35.3Chemosensors 11 00136 i0042.8Chemosensors 11 00136 i01014.9Chemosensors 11 00136 i017
Day 44.07Chemosensors 11 00136 i0053.66Chemosensors 11 00136 i01120Chemosensors 11 00136 i018
Day 58.14Chemosensors 11 00136 i0062.92Chemosensors 11 00136 i01223.27Chemosensors 11 00136 i019
Day 69.25Chemosensors 11 00136 i0074.17Chemosensors 11 00136 i01327.38Chemosensors 11 00136 i020
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Dimitrušev, N.; Nedeljko, P.; Mabes Raj, A.F.P.A.; Lobnik, A. Comparison of Surface and Spectral Properties of Optical Sensor Layers Prepared by Spin/Spray Coating and Printing Techniques. Chemosensors 2023, 11, 136. https://doi.org/10.3390/chemosensors11020136

AMA Style

Dimitrušev N, Nedeljko P, Mabes Raj AFPA, Lobnik A. Comparison of Surface and Spectral Properties of Optical Sensor Layers Prepared by Spin/Spray Coating and Printing Techniques. Chemosensors. 2023; 11(2):136. https://doi.org/10.3390/chemosensors11020136

Chicago/Turabian Style

Dimitrušev, Nena, Polonca Nedeljko, A. F. P. Allwin Mabes Raj, and Aleksandra Lobnik. 2023. "Comparison of Surface and Spectral Properties of Optical Sensor Layers Prepared by Spin/Spray Coating and Printing Techniques" Chemosensors 11, no. 2: 136. https://doi.org/10.3390/chemosensors11020136

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