Next Article in Journal
CSF Proteomic Alzheimer’s Disease-Predictive Subtypes in Cognitively Intact Amyloid Negative Individuals
Next Article in Special Issue
Comparison of Different Label-Free Techniques for the Semi-Absolute Quantification of Protein Abundance
Previous Article in Journal
Evaluation of the Sensitivity of Proteomics Methods Using the Absolute Copy Number of Proteins in a Single Cell as a Metric
Previous Article in Special Issue
A Novel Urinary Proteomics Classifier for Non-Invasive Evaluation of Interstitial Fibrosis and Tubular Atrophy in Chronic Kidney Disease
Article

Impact of Exposure to Chronic Light–Dark Phase Shifting Circadian Rhythm Disruption on Muscle Proteome in Periparturient Dairy Cows

1
Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA
2
Purdue Proteomics Facility, Bindley Bioscience Center, Purdue University, West Lafayette, IN 47907, USA
3
Department of Comparative Pathobiology, Purdue University, West Lafayette, IN 47907, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Jacek R. Wisniewski
Proteomes 2021, 9(3), 35; https://doi.org/10.3390/proteomes9030035
Received: 14 June 2021 / Revised: 10 July 2021 / Accepted: 13 July 2021 / Published: 29 July 2021
(This article belongs to the Special Issue Mass Spectrometry-Based Quantitative Proteomics)
Muscle tissue serves as a key nutrient reservoir that dairy cows utilize to meet energy and amino acid requirements for fetal growth and milk production. Circadian clocks act as homeostatic regulators so that organisms can anticipate regular environmental changes. The objective of this study was to use liquid chromatography tandem mass spectrometry (LC-MS/MS) to determine how chronic circadian disruption in late gestation affected the muscle tissue proteome. At five weeks before expected calving (BEC), multiparous Holstein cows were assigned to either a control (CON, n = 8) or a 6 h forward phase shift (PS, n = 8) of the light–dark cycle every 3 days. At calving, all animals were exposed to CON light–dark cycles. Muscle biopsies were collected from longissimus dorsi muscles at 21 days BEC and at 21 days postpartum (PP). At p < 0.1, 116 and 121 proteins were differentially abundant between PS and CON at 21 days BEC and 21 days PP, respectively. These proteins regulate beta oxidation and glycolysis. Between pregnancy and lactation, 134 and 145 proteins were differentially abundant in CON and PS cows, respectively (p < 0.1). At both timepoints, PS cows exhibited an oxidative stress signature. Thus, dairy cattle management strategies that minimize circadian disruptions may ensure optimal health and production performance. View Full-Text
Keywords: transition cow; oxidative stress; mitochondrial oxidation transition cow; oxidative stress; mitochondrial oxidation
Show Figures

Figure 1

MDPI and ACS Style

McCabe, C.J.; Aryal, U.K.; Casey, T.; Boerman, J. Impact of Exposure to Chronic Light–Dark Phase Shifting Circadian Rhythm Disruption on Muscle Proteome in Periparturient Dairy Cows. Proteomes 2021, 9, 35. https://doi.org/10.3390/proteomes9030035

AMA Style

McCabe CJ, Aryal UK, Casey T, Boerman J. Impact of Exposure to Chronic Light–Dark Phase Shifting Circadian Rhythm Disruption on Muscle Proteome in Periparturient Dairy Cows. Proteomes. 2021; 9(3):35. https://doi.org/10.3390/proteomes9030035

Chicago/Turabian Style

McCabe, Conor J., Uma K. Aryal, Theresa Casey, and Jacquelyn Boerman. 2021. "Impact of Exposure to Chronic Light–Dark Phase Shifting Circadian Rhythm Disruption on Muscle Proteome in Periparturient Dairy Cows" Proteomes 9, no. 3: 35. https://doi.org/10.3390/proteomes9030035

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop