Short-Chain Fatty Acids Modulate Metabolic Pathways and Membrane Lipids in Prevotella bryantii B14
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cultivation in Medium M2-A for Proteome Analysis
2.2. Cultivation for Long-Chain Fatty Acid Determination
2.3. Glucose Based Medium M2-B
2.4. Short-Chain Fatty Acid Determination
2.5. Long-Chain Fatty Acid Determination
2.6. Protein Extraction
2.7. In Gel Protein Digestion
2.8. LC-ESI-MS/MS
2.9. Data Analysis for Proteomics
3. Results
3.1. Cultivation of P. bryantii B14 Using Single SCFAs
3.2. Proteome Inventory of P. bryantii B14
3.3. Effect of SCFA Exposure on Long-Chain Fatty Acids in Membranes from P. bryantii
3.4. Effect of Carbon Source on Growth of P. bryantii
4. Discussion
4.1. SCFAs Replace Rumen Fluid for Cultivation of P. bryantii B14
4.2. SCFA Influence the Abundance of Iron Transport Proteins
4.3. SCFAs are Incorporated into Long-Chain Fatty Acids of Lipid Membranes
4.4. Isovaleric Acid Enhance Branched-Chain Amino Acid Synthesis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Cultivation Condition | Mean ± SD of OD600 | ∆pH | Production/Consumption (mM) of ____ | |||||||
---|---|---|---|---|---|---|---|---|---|---|
C2 | C3 | iC4 | C4 | iC5 | C5 | |||||
8 h incubation | Acet | 1.44 | ±0.19 | 1.34 | 9.55 | 0.00 | 0.00 | 0.00 | 0.06 | 0.00 |
Prop | 1.50 | ±0.17 | 1.32 | 10.03 | 0.70 | 0.00 | −0.04 | 0.08 | 0.07 | |
But | 1.43 | ±0.16 | 1.33 | 10.14 | −0.05 | 0.00 | −0.10 | 0.09 | 0.00 | |
iBut | 1.23 | ±0.07 | 0.85 | 6.06 | 0.00 | −0.49 | 0.00 | 0.08 | 0.00 | |
Val | 1.73 | ±0.04 | 1.47 | 11.77 | 0.00 | 0.00 | −0.03 | 0.16 | −0.69 | |
iVal | 1.16 | ±0.09 | 0.56 | 6.21 | 0.00 | 0.00 | 0.00 | −0.24 | 0.00 | |
Cultivation Condition | Mean ± SD of OD600 | ∆pH | Production/Consumption (mM) of ____ | |||||||
C2 | C3 | iC4 | C4 | iC5 | C5 | |||||
24 h incubation | Acet | 1.67 | ±0.04 | 1.38 | 13.94 | 0.00 | 0.00 | 0.00 | 0.08 | 0.00 |
Prop | 1.64 | ±0.26 | 1.57 | 16.05 | 0.27 | 0.00 | −0.04 | 0.07 | 0.06 | |
But | 1.66 | ±0.15 | 1.61 | 16.79 | −0.10 | 0.00 | −0.43 | 0.10 | 0.00 | |
iBut | 1.64 | ±0.06 | 1.49 | 14.02 | 0.00 | −0.87 | 0.00 | 0.09 | 0.00 | |
Val | 1.77 | ±0.13 | 1.62 | 15.59 | 0.00 | 0.00 | −0.01 | 0.17 | −0.89 | |
iVal | 1.54 | ±0.34 | 1.05 | 10.82 | 0.00 | 0.00 | 0.00 | −0.46 | 0.00 |
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Trautmann, A.; Schleicher, L.; Deusch, S.; Gätgens, J.; Steuber, J.; Seifert, J. Short-Chain Fatty Acids Modulate Metabolic Pathways and Membrane Lipids in Prevotella bryantii B14. Proteomes 2020, 8, 28. https://doi.org/10.3390/proteomes8040028
Trautmann A, Schleicher L, Deusch S, Gätgens J, Steuber J, Seifert J. Short-Chain Fatty Acids Modulate Metabolic Pathways and Membrane Lipids in Prevotella bryantii B14. Proteomes. 2020; 8(4):28. https://doi.org/10.3390/proteomes8040028
Chicago/Turabian StyleTrautmann, Andrej, Lena Schleicher, Simon Deusch, Jochem Gätgens, Julia Steuber, and Jana Seifert. 2020. "Short-Chain Fatty Acids Modulate Metabolic Pathways and Membrane Lipids in Prevotella bryantii B14" Proteomes 8, no. 4: 28. https://doi.org/10.3390/proteomes8040028