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In Silico Identification of Antimicrobial Peptides in the Proteomes of Goat and Sheep Milk and Feta Cheese

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The Cyprus Institute of Neurology & Genetics, Bioinformatics Group, 6 International Airport Avenue, 2370 Nicosia, Cyprus, P.O. Box 23462, 1683 Nicosia, Cyprus
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The Cyprus School of Molecular Medicine, 6 International Airport Avenue, 2370 Nicosia, Cyprus, P.O. Box 23462, 1683 Nicosia, Cyprus
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Proteomics Research Unit, Biomedical Research Foundation of the Academy of Athens, 115 27 Athens, Greece
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Author to whom correspondence should be addressed.
Proteomes 2019, 7(4), 32; https://doi.org/10.3390/proteomes7040032
Received: 8 August 2019 / Revised: 16 September 2019 / Accepted: 19 September 2019 / Published: 21 September 2019
(This article belongs to the Special Issue Top-down Proteomics: In Memory of Dr. Alfred Yergey)
Milk and dairy products are a major functional food group of growing scientific and commercial interest due to their nutritional value and bioactive “load”. A major fraction of the latter is attributed to milk’s rich protein content and its biofunctional peptides that occur naturally during digestion. On the basis of the identified proteome datasets of milk whey from sheep and goat breeds in Greece and feta cheese obtained during previous work, we applied an in silico workflow to predict and characterise the antimicrobial peptide content of these proteomes. We utilised existing tools for predicting peptide sequences with antimicrobial traits complemented by in silico protein cleavage modelling to identify frequently occurring antimicrobial peptides (AMPs) in the gastrointestinal (GI) tract in humans. The peptides of interest were finally assessed for their stability with respect to their susceptibility to cleavage by endogenous proteases expressed along the intestinal part of the GI tract and ranked with respect to both their antimicrobial and stability scores. View Full-Text
Keywords: peptidomics; proteomics; antimicrobial peptides; milk whey; proteolysis; proteases; in silico digestion; functional foods; gastrointestinal tract peptidomics; proteomics; antimicrobial peptides; milk whey; proteolysis; proteases; in silico digestion; functional foods; gastrointestinal tract
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Tomazou, M.; Oulas, A.; Anagnostopoulos, A.K.; Tsangaris, G.T.; Spyrou, G.M. In Silico Identification of Antimicrobial Peptides in the Proteomes of Goat and Sheep Milk and Feta Cheese. Proteomes 2019, 7, 32.

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