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Open AccessFeature PaperArticle

Proteome Profiling of Diabetic Mellitus Patient Urine for Discovery of Biomarkers by Comprehensive MS-Based Proteomics

Biofluid Biomarker Center, Niigata University, Niigata 950-2181, Japan
Department of Pediatrics, National Hospital Organization Niigata Hospital, Kashiwazaki 945-8585, Japan
Department of Nephrology, Shinrakuen Hospital, Niigata 950-2087, Japan
Author to whom correspondence should be addressed.
Proteomes 2018, 6(1), 9;
Received: 27 December 2017 / Revised: 19 January 2018 / Accepted: 2 February 2018 / Published: 6 February 2018
(This article belongs to the Special Issue Proteomic Biomarkers in Human Diseases)
Diabetic mellitus (DM) is a disease that affects glucose homeostasis and causes complications, such as diabetic nephropathy (DN). For early diagnosis of DN, microalbuminuria is currently one of the most frequently used biomarkers. However, more early diagnostic biomarkers are desired in addition to microalbuminuria. In this study, we performed comprehensive proteomics analysis of urine proteomes of diabetic mellitus patients without microalbuminuria and healthy volunteers to compare the protein profiles by mass spectrometry. With high confidence criteria, 942 proteins in healthy volunteer urine and 645 proteins in the DM patient urine were identified with label-free semi-quantitation, respectively. Gene ontology and pathway analysis were performed with the proteins, which were up- or down-regulated in the DM patient urine to elucidate significant changes in pathways. The discovery of a useful biomarker for early DN discovery is expected. View Full-Text
Keywords: urine; biomarker; diabetic mellitus urine; biomarker; diabetic mellitus
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Hirao, Y.; Saito, S.; Fujinaka, H.; Miyazaki, S.; Xu, B.; Quadery, A.F.; Elguoshy, A.; Yamamoto, K.; Yamamoto, T. Proteome Profiling of Diabetic Mellitus Patient Urine for Discovery of Biomarkers by Comprehensive MS-Based Proteomics. Proteomes 2018, 6, 9.

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