Next Article in Journal / Special Issue
MetaGOmics: A Web-Based Tool for Peptide-Centric Functional and Taxonomic Analysis of Metaproteomics Data
Previous Article in Journal
Proteomic Contributions to Medicinal Plant Research: From Plant Metabolism to Pharmacological Action
Open AccessFeature PaperArticle

Multi-Omic Biogeography of the Gastrointestinal Microbiota of a Pre-Weaned Lamb

Porto Conte Ricerche, Science and Technology Park of Sardinia, Tramariglio, 07041 Alghero, Italy
Department of Biomedical Sciences, University of Sassari, 07100 Sassari, Italy
Department of Agricultural Science, University of Sassari, 07100 Sassari, Italy
Author to whom correspondence should be addressed.
Proteomes 2017, 5(4), 36;
Received: 17 November 2017 / Revised: 12 December 2017 / Accepted: 14 December 2017 / Published: 18 December 2017
(This article belongs to the Special Issue Metaproteomics)
The digestive functions of the pre-weaned lamb gastrointestinal tracts (GITs) have been the subject of much research in recent years, but the microbial and host functions underlying these complex processes remain largely unknown. Here, we undertook a proof-of-principle metaproteogenomic investigation on luminal and mucosal samples collected from 10 GITs of a 30-day-old pre-weaned lamb. We demonstrate that the analysis of the diverse ecological niches along the GITs can reveal microbiota composition and metabolic functions, although low amounts of microbial proteins could be identified in the small intestinal and mucosal samples. Our data suggest that a 30-day lamb has already developed mature microbial functions in the forestomachs, while the effect of the milky diet appears to be more evident in the remaining GITs. We also report the distribution and the relative abundance of the host functions, active at the GIT level, with a special focus on those involved in digestive processes. In conclusion, this pilot study supports the suitability of a metaproteogenomic approach to the characterization of microbial and host functions of the lamb GITs, opening the way to further studies aimed at investigating the impact of early dietary interventions on the GIT microbiota of small ruminants. View Full-Text
Keywords: metaproteomics; microbial community; mucosa; ruminant; sheep metaproteomics; microbial community; mucosa; ruminant; sheep
Show Figures

Figure 1

MDPI and ACS Style

Palomba, A.; Tanca, A.; Fraumene, C.; Abbondio, M.; Fancello, F.; Atzori, A.S.; Uzzau, S. Multi-Omic Biogeography of the Gastrointestinal Microbiota of a Pre-Weaned Lamb. Proteomes 2017, 5, 36.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop