Genome-Wide Identification and Expression Analysis of the Strawberry FvbZIP Gene Family and the Role of Key Gene FabZIP46 in Fruit Resistance to Gray Mold
Abstract
:1. Introduction
2. Results and Analysis
2.1. Members of the bZIP Transcription Factor Family in Strawberries
2.2. Phylogenetic Relationships among the Family Members of bZIP Transcription Factors in Strawberries
2.3. Chromosomal Localization and Analysis of the Gene Structure of Strawberry bZIP Genes
2.4. Response of the FabZIP Genes of Fragaria × Ananassa “Benihoppe” Strawberry to Stress Caused by B. Cinerea
2.5. Analysis of the Transient Expression of FabZIP46 in Strawberries
2.6. Effect of Overexpression and Silencing of FabZIP46 on the Expression of Disease-Resistant Genes
3. Discussion
4. Materials and Methods
4.1. Fungi and Plant Materials
4.2. Extraction of FvbZIP Genes from Strawberries and Prediction of Physicochemical Properties
4.3. Phylogenetic Analysis and Sequence Alignment of Strawberry FvbZIP Genes
4.4. Analysis of the Structure of FvbZIP Genes in the Strawberry and Analysis of the MEME Protein Motif
4.5. Real-Time Fluorescence Quantitative Analysis of Gene Expression
4.6. Gene Cloning and Vector Construction
4.7. Transient Transformation of Strawberries and Experimental Design
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Lu, B.; Wang, Y.; Zhang, G.; Feng, Y.; Yan, Z.; Wu, J.; Chen, X. Genome-Wide Identification and Expression Analysis of the Strawberry FvbZIP Gene Family and the Role of Key Gene FabZIP46 in Fruit Resistance to Gray Mold. Plants 2020, 9, 1199. https://doi.org/10.3390/plants9091199
Lu B, Wang Y, Zhang G, Feng Y, Yan Z, Wu J, Chen X. Genome-Wide Identification and Expression Analysis of the Strawberry FvbZIP Gene Family and the Role of Key Gene FabZIP46 in Fruit Resistance to Gray Mold. Plants. 2020; 9(9):1199. https://doi.org/10.3390/plants9091199
Chicago/Turabian StyleLu, Bei, Yuanhua Wang, Geng Zhang, Yingna Feng, Zhiming Yan, Jianhua Wu, and Xuehao Chen. 2020. "Genome-Wide Identification and Expression Analysis of the Strawberry FvbZIP Gene Family and the Role of Key Gene FabZIP46 in Fruit Resistance to Gray Mold" Plants 9, no. 9: 1199. https://doi.org/10.3390/plants9091199