Detection of Embryonic Suspensor Cell Death by Whole-Mount TUNEL Assay in Tobacco
Abstract
:1. Introduction
2. Results
2.1. Preparation of Hand-Made Tools
2.2. Collection of Living Embryos
2.3. TUNEL Assay
2.3.1. Fix the Embryos
2.3.2. Permeabilize and Equilibrate the Embryos
2.3.3. Label the Embryos
2.3.4. Analyze the Fluorescence
3. Materials and Equipment
3.1. Plant Materials
3.2. Reagents
3.3. Equipment
3.4. Solutions
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Shi, C.; Luo, P.; Zhao, P.; Sun, M.-X. Detection of Embryonic Suspensor Cell Death by Whole-Mount TUNEL Assay in Tobacco. Plants 2020, 9, 1196. https://doi.org/10.3390/plants9091196
Shi C, Luo P, Zhao P, Sun M-X. Detection of Embryonic Suspensor Cell Death by Whole-Mount TUNEL Assay in Tobacco. Plants. 2020; 9(9):1196. https://doi.org/10.3390/plants9091196
Chicago/Turabian StyleShi, Ce, Pan Luo, Peng Zhao, and Meng-Xiang Sun. 2020. "Detection of Embryonic Suspensor Cell Death by Whole-Mount TUNEL Assay in Tobacco" Plants 9, no. 9: 1196. https://doi.org/10.3390/plants9091196