Iron-Sulfur Cluster Complex Assembly in the Mitochondria of Arabidopsis thaliana
Abstract
:1. Introduction
2. Results
2.1. Effect of Frataxin (AtFH) and AtISD11 on Desulfurase (AtNFS1) Kinetic Parameters
2.2. Pull-Down Assays and Interaction Studies between AtNFS1, AtFH, and AtISD11
2.3. Evaluation of the Attenuation of Fenton Reaction in the Presence of AtFH, AtISD11, and AtNFS1
3. Discussion
4. Materials and Methods
4.1. Cloning, Expression and Purification of AtNFS1, AtFH, and AtISD11
4.2. Cysteine Desulfurase Assay
4.3. Pull-Down Assays
4.4. Oxidative Degradation Assays
4.5. Additional Methods
4.6. Statistical Analyses
Author Contributions
Funding
Conflicts of Interest
References
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Armas, A.M.; Balparda, M.; Terenzi, A.; Busi, M.V.; Pagani, M.A.; Gomez-Casati, D.F. Iron-Sulfur Cluster Complex Assembly in the Mitochondria of Arabidopsis thaliana. Plants 2020, 9, 1171. https://doi.org/10.3390/plants9091171
Armas AM, Balparda M, Terenzi A, Busi MV, Pagani MA, Gomez-Casati DF. Iron-Sulfur Cluster Complex Assembly in the Mitochondria of Arabidopsis thaliana. Plants. 2020; 9(9):1171. https://doi.org/10.3390/plants9091171
Chicago/Turabian StyleArmas, Alejandro M., Manuel Balparda, Agustina Terenzi, Maria V. Busi, Maria A. Pagani, and Diego F. Gomez-Casati. 2020. "Iron-Sulfur Cluster Complex Assembly in the Mitochondria of Arabidopsis thaliana" Plants 9, no. 9: 1171. https://doi.org/10.3390/plants9091171