Next Article in Journal
Understory Vegetation Change Following Woodland Reduction Varies by Plant Community Type and Seeding Status: A Region-Wide Assessment of Ecological Benefits and Risks
Next Article in Special Issue
Innovative Extraction Techniques Using Deep Eutectic Solvents and Analytical Methods for the Isolation and Characterization of Natural Bioactive Compounds from Plant Material
Previous Article in Journal
Transcriptomic Analysis of a Susceptible African Maize Line to Fusarium verticillioides Infection
Article

Plukenetia huayllabambana Fruits: Analysis of Bioactive Compounds, Antibacterial Activity and Relative Action Mechanisms

1
CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 437004, China
2
Department of Biomedical Sciences, Faculty of Health Sciences, University of Buea, P.O. Box 63, Buea, Cameroon
3
Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 437004, China
4
Innovation Academy for Drug Discovery and Development, Chinese Academy of Sciences, Shanghai 201203, China
5
Centre for Natural Products Discovery, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool L3 3AF, UK
6
Unit of Research in Microbiology and Antimicrobial Substances/Laboratory of Cancer Research, Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon
*
Author to whom correspondence should be addressed.
Plants 2020, 9(9), 1111; https://doi.org/10.3390/plants9091111
Received: 5 August 2020 / Revised: 24 August 2020 / Accepted: 25 August 2020 / Published: 28 August 2020
(This article belongs to the Special Issue Plant Analytical Chemistry)
Plukenetia huayllabambana is an edible plant traditionally used to cure wounds and various infections. The present work assessed, for the first time, the antibacterial efficacy of solvent fractions from P. huayllabambana fruits. The crude methanol extract was obtained applying ultrasound-assisted extraction, followed by partitioning through successive depletion among solvents of increasing polarity to yield fractions (n-hexane, dichloromethane, ethyl acetate, and n-butanol). The minimal inhibitory concentration (MIC) was determined following antibacterial testing, using the broth microdilution technique against a panel of drug-resistant Gram-negative and Gram-positive bacteria. Possible modes of action of the most active fraction were also investigated. Gas chromatography–mass spectrometry (GC–MS) was used to identify phytocompounds that may account for the recorded activities. Methanol, n-hexane (PH-n-Hex), and ethyl acetate extracts inhibited 100% of studied bacteria, with the recorded MIC ranging from 0.125–1 mg/mL. PH-n-Hex appeared as the most active partition, exerting a bacteriostatic effect. PH-n-Hex probably acts by interfering with bacterial biofilm formation, proton pumps, and bacterial cell membrane integrity. The GC–MS analysis of PH-n-Hex led to the identification of 11 potentially bioactive components, including fatty acids, phytosterol, and diterpene alcohol as major ones. P. huayllabambana can be considered as a plant of pharmacological value—a source of potent anti-infective drug entities. View Full-Text
Keywords: Plukenetia huayllabambana; solvent fractions; antibacterial; modes of action; GC–MS Plukenetia huayllabambana; solvent fractions; antibacterial; modes of action; GC–MS
Show Figures

Figure 1

MDPI and ACS Style

Seukep, A.J.; Fan, M.; Sarker, S.D.; Kuete, V.; Guo, M.-Q. Plukenetia huayllabambana Fruits: Analysis of Bioactive Compounds, Antibacterial Activity and Relative Action Mechanisms. Plants 2020, 9, 1111. https://doi.org/10.3390/plants9091111

AMA Style

Seukep AJ, Fan M, Sarker SD, Kuete V, Guo M-Q. Plukenetia huayllabambana Fruits: Analysis of Bioactive Compounds, Antibacterial Activity and Relative Action Mechanisms. Plants. 2020; 9(9):1111. https://doi.org/10.3390/plants9091111

Chicago/Turabian Style

Seukep, Armel J., Minxia Fan, Satyajit D. Sarker, Victor Kuete, and Ming-Quan Guo. 2020. "Plukenetia huayllabambana Fruits: Analysis of Bioactive Compounds, Antibacterial Activity and Relative Action Mechanisms" Plants 9, no. 9: 1111. https://doi.org/10.3390/plants9091111

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop