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Article

Chlorophyll a Fluorescence Transient and 2-Dimensional Electrophoresis Analyses Reveal Response Characteristics of Photosynthesis to Heat Stress in Malus. ‘Prairifire’

1
Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China
2
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, China
*
Author to whom correspondence should be addressed.
Plants 2020, 9(8), 1040; https://doi.org/10.3390/plants9081040
Received: 18 July 2020 / Revised: 12 August 2020 / Accepted: 13 August 2020 / Published: 15 August 2020
(This article belongs to the Special Issue Responses of Plants to Environmental Stresses)
Flowering crabapples are a series of precious ornamental woody plants. However, their growth and development are inhibited in the subtropical regions due to the weak photosynthesis under high-temperature environment in the summer. Chlorophyll a fluorescence transient and 2-dimensional electrophoresis (2-DE) analyses were conducted to investigate the response characteristics of photosynthesis under simulated 38 °C heat stress in leaves of Malus. ‘Prairifire’, a spring-red leaf cultivar of flowering crabapple with strong thermal adaptability. In the present study, the net photosynthetic rate (Pn) was significantly decreased during the heat shock process, which showed a similar trend to the stomatal conductance (Gs), indicating a sensitive stomatal behavior to heat stress. Moreover, an efficient reaction center in photosystem II (PSII), and a functionally intact oxygen-evolving complex (OEC) conferred strong photosynthetic adaptability under heat stress. The higher level of transketolase (TK) under 48-h heat shock treatment was considered a protective mechanism of photosynthetic apparatus. However, heat stress inhibited the functions of light harvesting complex II (LHCII), electron transport in PSII, and the levels of key enzymes in the Calvin cycle, which were considered as the reasons causing an increase in the proportion of non-stomatal restrictions. View Full-Text
Keywords: heat stress; Malus. ‘Prairifire’; photosynthetic characteristics; chlorophyll a fluorescence; 2-dimensional electrophoresis heat stress; Malus. ‘Prairifire’; photosynthetic characteristics; chlorophyll a fluorescence; 2-dimensional electrophoresis
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MDPI and ACS Style

Wang, T.; Luo, S.; Ma, Y.; Li, L.; Xie, Y.; Zhang, W. Chlorophyll a Fluorescence Transient and 2-Dimensional Electrophoresis Analyses Reveal Response Characteristics of Photosynthesis to Heat Stress in Malus. ‘Prairifire’. Plants 2020, 9, 1040. https://doi.org/10.3390/plants9081040

AMA Style

Wang T, Luo S, Ma Y, Li L, Xie Y, Zhang W. Chlorophyll a Fluorescence Transient and 2-Dimensional Electrophoresis Analyses Reveal Response Characteristics of Photosynthesis to Heat Stress in Malus. ‘Prairifire’. Plants. 2020; 9(8):1040. https://doi.org/10.3390/plants9081040

Chicago/Turabian Style

Wang, Tao, Siqian Luo, Yingli Ma, Lingyu Li, Yinfeng Xie, and Wangxiang Zhang. 2020. "Chlorophyll a Fluorescence Transient and 2-Dimensional Electrophoresis Analyses Reveal Response Characteristics of Photosynthesis to Heat Stress in Malus. ‘Prairifire’" Plants 9, no. 8: 1040. https://doi.org/10.3390/plants9081040

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