Arabidopsis Plastid-RNA Polymerase RPOTp Is Involved in Abiotic Stress Tolerance
Abstract
:1. Introduction
2. Results
2.1. Knock-Down of RPOTp Alters NaCl, Mannitol and ABA Responses
2.2. Analysis of Gene Ontologies in the Transcriptome of the sca3-2 Mutant
2.3. RPOTp Expression is Controlled by Plastid Retrograde Signaling in Response to Salt Stress
2.4. Salt Stress Differentially Affects the Transcript Abundance of Plastid-Encoded Genes
2.5. ABA Down-Regulates the Expression of NEP and Genes Encoding PEP Subunits
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Germination and Growth Sensitivity Assays
4.3. Quantitative RT-PCR (qRT-PCR)
4.4. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Gene | Normalized Transcript Levels in Col-0 Plants Exposed to 100 mM NaCl Relative to Control Plants | ||
---|---|---|---|
Protein Product | Fold Change | p-Value | |
Nuclear genes | |||
RPOTp/SCA3 | NEP 1 RPOTp | 2.27 ± 0.84 | 4.113 × 10−5 ** |
RPOTmp | NEP 1 RPOTmp | 3.50 ± 1.70 | 3.996 × 10−4 ** |
RPOTm | NEP 1 RPOTm | 1.34 ± 0.33 | 0.036 * |
AOX1A | Alternative oxidase 1A | 3.90 ± 1.00 | 4.113 × 10−5 ** |
LHCB1 | Light-harvesting complex protein B1 | 0.70 ± 0.44 | 0.036 * |
mTERF5 | mTERF5 | 0.77 ± 0.31 | 0.008 ** |
mTERF9 | mTERF9 | 1.10 ± 0.30 | 0.015 * |
COR15B | Cold-regulated 15B | 4.76 ± 2.11 | 2.20 × 10−10 ** |
RD29A | Responsive to desiccation 29A | 2.74 ± 0.76 | 5.83 × 10−4 ** |
Chloroplast genes | |||
psaA | Photosystem I reaction center protein | 1.12 ± 0.49 | 0.709 |
psaB | Photosystem I reaction center protein | 1.04 ± 0.28 | 0.709 |
psbA | Chlorophyll binding protein D1 | 0.60 ± 0.22 | 4.113 × 10−5 ** |
clpP | ATP-dependent protease | 1.18 ± 0.27 | 0.036 * |
rps18 | Ribosomal protein S18 | 0.83 ± 0.23 | 0.709 |
rpoA | PEP 2 α subunit | 0.63 ± 0.27 | 0.001 ** |
rpoB | PEP 2 β subunit | 0.61 ± 0.09 | 0.002 ** |
rpoC1 | PEP 2 β’ subunit | 0.86 ± 0.20 | 0.220 |
accD | Carboxytransferase β subunit of the Acetyl-CoA carboxylase | 0.88 ± 0.35 | 0.709 |
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Lidón-Soto, A.; Núñez-Delegido, E.; Pastor-Martínez, I.; Robles, P.; Quesada, V. Arabidopsis Plastid-RNA Polymerase RPOTp Is Involved in Abiotic Stress Tolerance. Plants 2020, 9, 834. https://doi.org/10.3390/plants9070834
Lidón-Soto A, Núñez-Delegido E, Pastor-Martínez I, Robles P, Quesada V. Arabidopsis Plastid-RNA Polymerase RPOTp Is Involved in Abiotic Stress Tolerance. Plants. 2020; 9(7):834. https://doi.org/10.3390/plants9070834
Chicago/Turabian StyleLidón-Soto, Abel, Eva Núñez-Delegido, Iván Pastor-Martínez, Pedro Robles, and Víctor Quesada. 2020. "Arabidopsis Plastid-RNA Polymerase RPOTp Is Involved in Abiotic Stress Tolerance" Plants 9, no. 7: 834. https://doi.org/10.3390/plants9070834