Study of Androgenic Plant Families of Alloplasmic Introgression Lines (H. vulgare) –T. aestivum and the Use of Sister DH Lines in Breeding
Abstract
:1. Introduction
- -
- to determine how often the formation of regenerant families occurs in comparison with the development of single plantlets in the anther culture of bread wheat genotypes of different origin;
- -
- to characterize families of regenerants R0 by the level of fertility, which was manifested in plants as a result of spontaneous doubling of the number of chromosomes;
- -
- to study the association of reduced fertility in R1 regenerants with aneuploidy;
- -
- to evaluate the prospects of alloplasmic sister DH lines for further breeding based on field trials studying resistance to fungal pathogens and agronomic traits.
2. Results
2.1. Development of Embryo-Like Structures and Plant Regeneration
2.2. Efficiency of Development of Single Green Plantlets and Green Plantlet Families from ELS
2.3. Evaluation of Families and Androgenic R0 plants
2.4. Evaluation of Sister Line Families in R1
2.5. Evaluation of Agronomic Parameters and Resistance to Fungal Pathogens in Alloplasmic Sister DH Lines of Three Hybrid Combinations
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Anther Culture and Plant Regeneration
4.3. Acclimatized Anther Culture-Derived Regenerants
4.4. Analysis of the Results
4.5. Cytological and Molecular Analysis
4.6. Field Studies of Alloplasmic Sister DH Lines
4.7. Statistical Analyses
5. Conclusions
6. Patents
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Genotype | Number of ELS/100 Anthers | Number of all Plantlets/100 Anthers | Number of Green Plantlets/100 Anthers |
---|---|---|---|
Pyr28 | 57.53 ab | 29.23 ab | 7.00 a |
Om37 | 24.76 a | 12.20 a | 5.06 a |
Om38 | 8.93 a | 3.16 a | 0.20 a |
L-17(3) | 10.76 a | 16.60 a | 9.86 a |
L-311(4) | 124.46 c | 105.20 c | 61.76 c |
L-311(4) × Om37 | 65.51 b | 78.03 b | 42.73 bc |
Om37 × L-311(4) | 67.63 b | 60.33 b | 39.20 b |
L-311(4) × L-134 | 85.43 b | 36.76 ab | 20.33 ab |
L-311(4) × Om38 | 69.77 b | 47.70 b | 19.46 ab |
L-311(4) × 2870 | 46.05 ab | 51.23 b | 14.39 a |
Mean | 56.08 | 44.04 | 21.99 |
LSD5% = | 18.70 | 11.76 | 10.29 |
Genotype | Number of Single GPs/100 EPS (Mean ± SE) | Number of GP Families/100 EPS (Mean ± SE) | Number of GPs/Family | GP Variation in One Family |
---|---|---|---|---|
Pyr28 | 1.17 ± 0.33 a | 2.75 ± 0.51 b | 4.35 | 2–10 |
Om37 | 1.00 ± 0.44 a | 3.40 ± 0.81 b | 3.70 | 3–12 |
Om38 | 0 | 2.27 ± 1.58 ns | 2.00 | – |
L-17(3) | 1.20 ± 0.84 a | 7.22 ± 2.00 b | 5.61 | 2–22 |
L-311(4) | 0.95 ± 0.22 a | 5.11 ± 0.52 b | 8.83 | 2–31 |
L-311(4) × Om37 | 0.83 ± 0.37 a | 5.68 ± 0.94 b | 9.91 | 2–36 |
Om37 × L-311(4) | 1.83 ± 0.57 a | 8.42 ± 1.18 b | 6.78 | 2–20 |
L-311(4) × L-134 | 0.79 ± 0.29 a | 3.84 ± 0.64 b | 5.00 | 3–25 |
L-311(4) × Om38 | 1.91 ± 0.57 a | 7.15 ± 1.07 b | 5.14 | 3–28 |
L-311(4) × 2870 | 1.01 ± 0.41 a | 7.89 ± 1.11 b | 6.75 | 2–35 |
Medium | 1.07 ± 0.12 a | 5.37 ± 0.27 b | 5.81 | 2–36 |
Genotype | Number of Families with Green Plants | Frequency (%) Families with Androgenic Plants with Certain Level of Sterility/Fertility | |||
---|---|---|---|---|---|
S | PF | F | S + F and S + PF + F | ||
Pyr28 | 11 | 63.64 | 9.09 | 18.18 | 9.09 |
Om37 | 12 | 41.66 | 8.33 | 16.68 | 33.33 |
L-17(3) | 13 | 61.54 | 15.38 | 7.70 | 15.38 |
L-311(4) | 45 | 57.78 | 8.89 | 15.55 | 17.78 |
L-311(4) × Om37 | 20 | 45.00 | 10.00 | 25.00 | 20.00 |
Om37 × L-311(4) | 34 | 52.94 | 8.82 | 17.65 | 20.59 |
L-311(4) × L-134 | 18 | 44.44 | 5.56 | 27.78 | 22.22 |
L-311(4) × Om38 | 20 | 55.00 | 10.00 | 5.00 | 30.00 |
L-311(4) × 2870 | 47 | 23.40 | 8.51 | 38.30 | 29.79 |
Mean | 220 | 46.82 | 9.09 | 20.00 | 24.09 |
Genotype | No. of Tested Plants | No. of Partially Fertile and Fertile Plants (DH) | Frequency (%) of Spontaneous Rediploidization |
---|---|---|---|
Pyr28 | 48 | 14 | 29.17 |
Om37 | 44 | 10 | 22.72 |
L-17(3) | 86 | 38 | 44.18 |
L-311(4) | 297 | 136 | 45.79 |
L-311(4) × Om37 | 198 | 67 | 33.83 |
Om37 × L-311(4) | 174 | 40 | 22.98 |
L-311(4) × L-134 | 85 | 20 | 23.52 |
L-311(4) × Om38 | 90 | 17 | 18.88 |
L-311(4) × L-2870 | 292 | 154 | 52.73 |
Mean | 1314 | 496 | 37.74 |
Genotype | No. of Families Tested | Frequency (%) of Families | ||
---|---|---|---|---|
Group I | Group II | Group III | ||
100% Fertile Plants in R1SL | 75–95% Fertile Plants in R1SL | Less than 50% of Fertile Plants in R1SL | ||
L-311(4) | 15 | 80.00 a | 13.33 b | 6.67 b |
L-311(4) × Om37 | 10 | 40.00 ab | 40.00 ab | 20.00 ab |
L-311(4) × L-134 | 8 | 37.50 ab | 25.00 ab | 37.50 ab |
L-311(4) × 2870 | 19 | 36.84 b | 47.37 a | 15.79 ab |
Mean of hybrid | 37 | 37.84 b | 40.54 a | 21.62 a |
Mean | 52 | 50.00 | 32.69 | 17.31 |
Genotype | Total Green Plants R1 Tested | Plants with Ploidy Level | |||
---|---|---|---|---|---|
Hexaploids | Aneuploids | ||||
No. | Frequency (%) | No. | Frequency (%) | ||
Fertile Plants from Group I and Group II | |||||
L-311(4) | 39 | 37 | 94.87 | 2 | 5.13 |
L-311(4) × Om37 | 31 | 28 | 90.32 | 3 | 9.68 |
L-311(4) × L-134 | 24 | 22 | 91.67 | 2 | 8.33 |
L-311(4) × 2870 | 48 | 41 | 85.42 | 7 | 14.58 |
Mean | 142 | 128 | 90.14 | 14 | 9.86 |
Partly Fertile Plants from Group II and Group III | |||||
L-311(4) | 14 | 2 | 14.29 | 12 | 85.71 |
L-311(4) × Om37 | 10 | 0 | 0 | 10 | 100 |
L-311(4) × L-134 | 11 | 1 | 9.09 | 10 | 90.91 |
L-311(4) × 2870 | 15 | 4 | 26.67 | 11 | 73.33 |
Mean | 50 | 7 | 14.00 | 43 | 86.00 |
Genotype, Families, Sister DH lines | The Presence of Fungal Pathogen Resistance Genes in Plants R1, Sources of DH Lines | Number of Plants of DH Lines Reached Heading/Number of Seeds Sown (%) | Resistance to Fungal Pathogens | |||
---|---|---|---|---|---|---|
Lr26/Sr31 | Lr19/Sr25 | Leaf Rust | Stem Rust | Powdery Mildew | ||
L-311(4) × Om37 | ||||||
Family 1 | ||||||
R16-DH1 | + | + | 84.44 b | 0 | 0 | MS |
R16-DH2 | + | + | 82.22 b | R | 0 | MR |
R16-DH3 | + | + | 86.67 b | 0 | 0 | MR |
R16-DH4 | + | + | 88.89 b | 0 | 0 | MS |
Family 2 | ||||||
R17-DH1 | + | + | 84.44 b | 0 | 0 | MS |
R17-DH2 | + | + | 88.89 b | 0 | 0 | MR |
R17-DH3 | + | + | 75.55 b | R | 0 | MS |
R17-DH4 | + | + | 86.67 b | 0 | 0 | MR |
Family 3 | ||||||
R18-DH1 | + | + | 46.67 a | R | 0 | MS |
R18-DH2 | + | + | 51.11 a | 0 | 0 | MS |
R18-DH3 | + | + | 44.44 a | 0 | 0 | MS |
R18-DH4 | + | + | 48.89 a | 0 | 0 | MS |
R18-DH5 | + | + | 42.22 a | 0 | 0 | MS |
R18-DH6 | + | + | 73.33 b | R | R | MS |
L-311(4) × L-134 | ||||||
Family 1 | ||||||
R26-DH1 | + | + | 82.22 b | R | 0 | MS |
R26-DH2 | + | + | 86.67 b | R | 0 | MS |
R26-DH3 | + | + | 84.44 b | 0 | 0 | MR |
R26-DH4 | + | + | 80.00 b | R | 0 | MS |
Family 2 | ||||||
R27-DH1 | + | + | 82.22 b | 0 | 0 | MR |
R27-DH2 | + | + | 73.33 b | 0 | 0 | MS |
R27-DH3 | + | + | 75.55 b | 0 | 0 | MS |
Family 3 | ||||||
R28-DH1 | + | + | 86.67 b | 0 | 0 | R |
R28-DH2 | + | + | 88.89 b | 0 | 0 | R |
L-311(4) × 2870 | ||||||
Family 1 | ||||||
R51-DH1 | + | - | 88.89 b | 0 | 0 | 0 |
R51-DH2 | + | - | 84.44 b | 0 | 0 | 0 |
R51-DH3 | + | - | 86.67 b | 0 | 0 | 0 |
R51-DH4 | + | - | 88.89 b | 0 | 0 | 0 |
Family 2 | ||||||
R52-DH1 | + | - | 82.22 b | MS | 0 | MS |
R52-DH2 | + | - | 77.77 b | MS | 0 | MS |
R52-DH3 | + | - | 84.44 b | MS | 0 | MS |
Family 3 | ||||||
R53-DH1 | + | - | 82.22 b | MS | 0 | MS |
R53-DH2 | + | - | 86.67 b | MS | 0 | MS |
R53-DH3 | + | - | 84.44 b | MS | 0 | MS |
R53-DH4 | + | - | 80.00 b | MS | 0 | MS |
Controls | ||||||
Omskaya 33 | - | - | 80.00 b | HS | HS | S |
Om37 | + | + | 86.66 b | 0 | 0 | MS |
L-311(4) | + | - | 88.88 b | MS | 0 | MS |
Genotype and Sister DH Lines | Heading Date | Yield (t/ha) | 1000-Grain Weight, (g) | Protein Content, (%) | Resistance to Fungal Pathogens | ||
---|---|---|---|---|---|---|---|
Lea fRust | Stem Rust | Powdery Mildew | |||||
L-311(4) × Om37 | |||||||
Family 1 | |||||||
R16-DH1 DH1 | 102 c | 3.01 ab | - | - | 0 | 0 | MS |
R16-DH2 | 99 c | 3.12 ab | - | - | 0 | 0 | MS |
R16-DH3 | 100 c | 2.60 a | - | - | R | 0 | MR |
R16-DH4 | 98 c | 2.98 ab | - | - | 0 | 0 | MS |
Family 2 | |||||||
R17-DH1 | 90 a | 4.14 ab | 36.00 bc | 16.53 c | 0 | R | MR |
R17-DH2 | 89 a | 4.87 ab | 35.45 bc | 16.02 bc | R | 0 | MS |
R17-DH3 | 91 a | 4.46 ab | 38.40 bc | 17.13 c | 0 | R | MS |
R17-DH4 | 90 a | 4.90 ab | 37.65 bc | 16.13 bc | 0 | 0 | MR |
L-311(4) × L-134 | |||||||
R28-DH1 | 94 b | 4.15 ab | 42.40 c | 13.96 b | 0 | 0 | MR |
R28-DH2 | 96 bc | 4.30 ab | 37.20 bc | 14.00 b | 0 | 0 | MR |
L-311(4) × 2870 | |||||||
R51-DH1 | 93 ab | 4.94 ab | 39.50 c | 14.42 bc | 0 | 0 | 0 |
R51-DH2 | 92 ab | 5.03 b | 39.50 c | 15.76 bc | 0 | 0 | 0 |
R51-DH3 | 94 b | 5.14 b | 36.80 bc | 14.31 bc | 0 | 0 | 0 |
R51-DH4 | 95 bc | 4.89 ab | 39.40 c | 16.13 bc | 0 | 0 | 0 |
Controls | |||||||
Omskaya 33 | 94 b | 3.27 ab | 23.00 a | 11.23 a | HS | HS | S |
Om37 | 96 bc | 4.58 ab | 33.00 bc | 14.29 bc | 0 | 0 | MS |
L-311(4) | 93 ab | 4.78 ab | 33.20 b | 14.19 bc | MS | 0 | S |
LSD5%= | 2 | 1.21 | 2.76 | 1.25 |
Genotype and Sister DH Lines | Heading Date | Yield (t/ha) | 1000-Grain Weight, (g) | Protein Content, (%) | Resistance by Fungal Pathogens | ||
---|---|---|---|---|---|---|---|
Leaf Rust | Stem Rust | Powdery Mildew | |||||
L-311(4) × Om37 | |||||||
R17-DH1 | 89 ab | 4.56 a | 35.9 ab | 15.57 ab | 0 | R | MR |
R17-DH2 | 90 ab | 4.74 a | 38.40 b | 17.13 b | R | R | MS |
R17-DH3 | 88 a | 4.80 a | 36.70 ab | 15.73 ab | 0 | R | MR |
R17-DH4 | 92 ab | 5.03 a | 39.90 bc | 15.16 ab | 0 | 0 | MR |
L-311(4) × L-134 | |||||||
R28-DH1 | 93 ab | 4.66 a | 40.70 bc | 16.10 b | R | 0 | 0 |
R28-DH2 | 95 b | 4.80 a | 39.70 bc | 16.19 b | R | 0 | 0 |
L-311(4) × 2870 | |||||||
R51-DH1 | 97 b | 5.07 a | 39.90 bc | 16.90 b | 0 | 0 | R |
R51-DH2 | 98 b | 4.76 a | 37.00 a | 16.40‘b | 0 | 0 | R |
R51-DH3 | 96 b | 4.97 a | 39.80 bc | 17.56 b | 0 | 0 | 0 |
R51-DH4 | 98 b | 5.12 a | 39.40 b | 17.11 b | 0 | 0 | 0 |
Controls | |||||||
Omskaya33 | 92 ab | 2.78 a | 33.10 a | 13.67 a | HS | HS | MS |
Om37 | 96 b | 3.80 a | 34.80 a | 15.61 ab | R | R | MS |
L-311(4) | 94 ab | 5.12 a | 44.20 c | 15.40 ab | MR | 0 | MS |
LSD5%= | 3 | 1.38 | 2.35 | 1.14 |
Genotype and Sister DH Lines | Heading Date | Yield (t/ha) | 1000-Grain Weight, (g) | Protein Content, (%) | Resistance to Fungal Pathogens | ||
---|---|---|---|---|---|---|---|
Leaf Rust | Stem Rust | Powdery Mildew | |||||
L-311(4) × Om37 | |||||||
R17-DH1 | 97 ab | 4.51 ab | 38.4 b | 17.31 b | R | R | MR |
R17-DH2 | 94 a | 4.75 ab | 38.4 b | 15.73 a | 0 | 0 | MR |
R17-DH3 | 94 a | 4.73 ab | 36.1 b | 17.10 b | 0 | 0 | MR |
R17-DH4 | 94 a | 5.02 b | 39.9 b | 15.16 a | 0 | 0 | MR |
L-311(4) × L-134 | |||||||
R28-DH1 | 102 b | 4.20 ab | 39.4 b | 16.70 b | 0 | 0 | R |
R28-DH2 | 98 ab | 4.55 ab | 44.5 c | 16.53 ab | 0 | 0 | R |
L-311(4) × 2870 | |||||||
R51-DH1 | 90 a | 4.72 ab | 40.4 b | 16.71 b | 0 | 0 | 0 |
R51-DH2 | 91 a | 5.08 b | 40.3 b | 17.61 b | 0 | 0 | 0 |
R51-DH3 | 91 a | 4.98 b | 39.2 b | 17.43 b | 0 | 0 | 0 |
R51-DH4 | 92 a | 5.00 b | 38.9 b | 17.56 b | 0 | 0 | 0 |
Controls | |||||||
Omskaya 33 | 93 a | 3.53 a | 24.8 a | 14.36 a | HS | HS | S |
Om37 | 96 ab | 4.40 ab | 37.9 b | 15.56 a | 0 | 0 | MR |
L-311(4) | 94 a | 4.63 ab | 38.4 b | 16.02 ab | MS | 0 | S |
LSD5%= | 3.0 | 0.62 | 2.33 | 1.10 |
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Pershina, L.; Trubacheeva, N.; Badaeva, E.; Belan, I.; Rosseeva, L. Study of Androgenic Plant Families of Alloplasmic Introgression Lines (H. vulgare) –T. aestivum and the Use of Sister DH Lines in Breeding. Plants 2020, 9, 764. https://doi.org/10.3390/plants9060764
Pershina L, Trubacheeva N, Badaeva E, Belan I, Rosseeva L. Study of Androgenic Plant Families of Alloplasmic Introgression Lines (H. vulgare) –T. aestivum and the Use of Sister DH Lines in Breeding. Plants. 2020; 9(6):764. https://doi.org/10.3390/plants9060764
Chicago/Turabian StylePershina, Lidiya, Nataliya Trubacheeva, Ekaterina Badaeva, Igor Belan, and Ludmila Rosseeva. 2020. "Study of Androgenic Plant Families of Alloplasmic Introgression Lines (H. vulgare) –T. aestivum and the Use of Sister DH Lines in Breeding" Plants 9, no. 6: 764. https://doi.org/10.3390/plants9060764