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Breeding Maize Maternal Haploid Inducers
Open AccessArticle

Development of In Vivo Haploid Inducer Lines for Screening Haploid Immature Embryos in Maize

National Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Yuanmingyuan West Road, Haidian District, Beijing 100193, China
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Plants 2020, 9(6), 739; https://doi.org/10.3390/plants9060739
Received: 2 April 2020 / Revised: 17 May 2020 / Accepted: 27 May 2020 / Published: 12 June 2020
(This article belongs to the Special Issue Doubled Haploid Technology in Plant Breeding)
Doubled haploid technology is widely applied in maize. The haploid inducer lines play critical roles in doubled haploid breeding. We report the development of specialized haploid inducer lines that enhance the purple pigmentation of crossing immature embryos. During the development of haploid inducer lines, two breeding populations derived from the CAU3/S23 and CAU5/S23 were used. Molecular marker-assisted selection for both qhir1 and qhir8 was used from BC1F1 to BC1F4. Evaluation of the candidate individuals in each generation was carried out by pollinating to the tester of ZD958. Individuals with fast and clear pigmentation of the crossing immature embryos, high number of haploids per ear, and high haploid induction rate were considered as candidates. Finally, three new haploid inducer lines (CS1, CS2, and CS3) were developed. The first two (CS1 and CS2) were from the CAU3/S23, with a haploid induction rate of 8.29%–13.25% and 11.54%–15.54%, respectively. Meanwhile, the CS3 was from the CAU5/S23. Its haploid induction rate was 8.14%–12.28%. In comparison with the donor haploid inducer lines, the 24-h purple embryo rates of the newly developed haploid inducer lines were improved by 10%–20%, with a ~90% accuracy for the identification of haploid immature embryos. These new haploid inducer lines will further improve the efficiency of doubled haploid breeding of maize. View Full-Text
Keywords: haploid induction rate; haploid immature embryo; haploid identification; maize haploid induction rate; haploid immature embryo; haploid identification; maize
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Chen, C.; Xiao, Z.; Zhang, J.; Li, W.; Li, J.; Liu, C.; Chen, S. Development of In Vivo Haploid Inducer Lines for Screening Haploid Immature Embryos in Maize. Plants 2020, 9, 739.

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