Freezing Tolerance and Expression of β-amylase Gene in Two Actinidia arguta Cultivars with Seasonal Changes

Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, China

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Plants 2020, 9(4), 515; https://doi.org/10.3390/plants9040515

Received: 30 March 2020 / Revised: 11 April 2020 / Accepted: 15 April 2020 / Published: 16 April 2020

(This article belongs to the Special Issue Abiotic Stress in Fruit Crops)

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Abstract

Low temperature causes injuries to plants during winter, thereby it affects kiwi fruit quality and yield. However, the changes in metabolites and gene expression during cold acclimation (CA) and deacclimation (DA) in kiwi fruit remain largely unknown. In this study, freezing tolerance, carbohydrate metabolism, and β-amylase gene expression in two Actinidia arguta cv. “CJ-1” and “RB-3” were detected from CA to DA stages. In all acclimation stages, the “CJ-1” was hardier than “RB-3” and possessed lower semi-lethal temperature (LT50). Furthermore, “CJ-1” had a more rapid acclimation speed than “RB-3”. Changes of starch, β-amylase, and soluble sugars were associated with freezing tolerance in both cultivars. Starch contents continued to follow a declining trend, while soluble sugars contents continuously accumulated in both cultivars during CA stages (from October to January). To investigate the possible molecular mechanism underlying cold response in A. arguta, in total, 16 AcBAMs genes for β-amylase were identified in the kiwi fruit genome. We carried out localization of chromosome, gene structure, the conserved motif, and the analysis of events in the duplication of genes from AcBAMs. Finally, a strong candidate gene named AaBAM3 from AcBAMs was cloned in Actinidia arguta (A. arguta), The real-time qPCR showed that AaBAM3 gene expression in seasonal changes was consistent with changes of soluble sugars. These results reveal that AaBAM3 may enhance the freezing tolerance of A. arguta through increasing soluble sugar content. View Full-Text

Keywords: kiwi fruit; freezing tolerance; electrolyte leakage; β-amylase gene

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MDPI and ACS Style

Sun, S.; Fang, J.; Lin, M.; Qi, X.; Chen, J.; Wang, R.; Li, Z.; Li, Y.; Muhammad, A. Freezing Tolerance and Expression of β-amylase Gene in Two Actinidia arguta Cultivars with Seasonal Changes. Plants 2020, 9, 515. https://doi.org/10.3390/plants9040515

AMA Style

Sun S, Fang J, Lin M, Qi X, Chen J, Wang R, Li Z, Li Y, Muhammad A. Freezing Tolerance and Expression of β-amylase Gene in Two Actinidia arguta Cultivars with Seasonal Changes. Plants. 2020; 9(4):515. https://doi.org/10.3390/plants9040515

Chicago/Turabian Style

Sun, Shihang, Jinbao Fang, Miaomiao Lin, Xiujuan Qi, Jinyong Chen, Ran Wang, Zhi Li, Yukuo Li, and Abid Muhammad. 2020. "Freezing Tolerance and Expression of β-amylase Gene in Two Actinidia arguta Cultivars with Seasonal Changes" Plants 9, no. 4: 515. https://doi.org/10.3390/plants9040515

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Freezing Tolerance and Expression of β-amylase Gene in Two Actinidia arguta Cultivars with Seasonal Changes

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