Genome-Wide Identification, Expression Analysis and Functional Study of CCT Gene Family in Medicago truncatula
Abstract
:1. Introduction
2. Results
2.1. Identification of CCT Genes in M. truncatula Genome
2.2. Analyses of Gene Structure, Conserved Domain and Phylogenetic Relationship of MtCCT Genes
2.3. Analyses of Chromosomal Distribution and Synteny of MtCCT Genes
2.4. Phylogenetic Analysis of CCT Proteins in Plants
2.5. Temporal and Spatial Expression Profiling of MtCCT Genes
2.6. Expression Patterns of MtCCT Genes under Light Circle
2.7. Expression Patterns of MtCCT Genes in Response to Hormones
2.8. Expression of MtCCT Genes Which Responded to Abiotic Stresses
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Identification of CCT Genes in M. truncatula
4.3. Analyses of Gene Structure and Conserved Motif
4.4. Analyses of Chromosomal Distribution, Synteny and Cis-Elements of MtCCT Genes
4.5. Phylogenetic Analysis of CCT Proteins in Representative Plants
4.6. RNA Extraction and Real-Time Quantitative PCR
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Gene | Chromosome | CDS (Coding Sequence) | Protein (aa) | Molecular Weight (D) | Theoretical Pi | Subfamily |
---|---|---|---|---|---|---|
MtCCT1 | Chr1 | Medtr1g008220.1 | 282 | 31927 | 4.94 | CMF |
MtCCT2 | Chr1 | Medtr1g013450.1 | 316 | 35496 | 6.31 | COL |
MtCCT3 | Chr1 | Medtr1g044785.1 | 230 | 25360 | 5.6 | CMF |
MtCCT4 | Chr1 | Medtr1g067110.1 | 748 | 81610 | 7.27 | PRR |
MtCCT5 | Chr1 | Medtr1g073350.1 | 414 | 45719 | 4.47 | CMF |
MtCCT6 | Chr1 | Medtr1g110870.1 | 436 | 48793 | 5.45 | COL |
MtCCT7 | Chr2 | Medtr2g068730.1 | 266 | 30816 | 5.21 | CMF |
MtCCT8 | Chr2 | Medtr2g088900.1 | 521 | 57571 | 5.85 | COL |
MtCCT9 | Chr2 | Medtr2g096080.1 | 251 | 28657 | 6.17 | CMF |
MtCCT10 | Chr3 | Medtr3g037390.1 | 575 | 64621 | 6.05 | PRR |
MtCCT11 | Chr3 | Medtr3g082630.2 | 411 | 45277 | 4.89 | COL |
MtCCT12 | Chr3 | Medtr3g091340.1 | 281 | 31916 | 5.23 | CMF |
MtCCT13 | Chr3 | Medtr3g092780.1 | 685 | 75851 | 6.11 | PRR |
MtCCT14 | Chr3 | Medtr3g100040.1 | 359 | 38847 | 4.47 | CMF |
MtCCT15 | Chr3 | Medtr3g100050.1 | 309 | 34206 | 5.62 | CMF |
MtCCT16 | Chr3 | Medtr3g105710.1 | 290 | 31869 | 7.11 | COL |
MtCCT17 | Chr4 | Medtr4g008090.1 | 121 | 13589 | 9.86 | CMF |
MtCCT18 | Chr4 | Medtr4g061360.1 | 796 | 87246 | 6.72 | PRR |
MtCCT19 | Chr4 | Medtr4g061823.1 | 242 | 27580 | 5.93 | CMF |
MtCCT20 | Chr4 | Medtr4g061910.1 | 242 | 27580 | 5.93 | CMF |
MtCCT21 | Chr4 | Medtr4g093730.1 | 334 | 37563 | 5.04 | CMF |
MtCCT22 | Chr4 | Medtr4g108880.1 | 585 | 68781 | 5.80 | PRR |
MtCCT23 | Chr4 | Medtr4g127420.1 | 379 | 42006 | 9.00 | CMF |
MtCCT24 | Chr4 | Medtr4g128930.1 | 375 | 41119 | 5.86 | COL |
MtCCT25 | Chr5 | Medtr5g010120.1 | 251 | 29195 | 5.83 | CMF |
MtCCT26 | Chr5 | Medtr5g066510.1 | 286 | 31424 | 5.53 | CMF |
MtCCT27 | Chr5 | Medtr5g069480.1 | 410 | 45027 | 5.70 | COL |
MtCCT28 | Chr5 | Medtr5g072780.1 | 217 | 25897 | 5.07 | CMF |
MtCCT29 | Chr7 | Medtr7g018170.1 | 396 | 43757 | 5.10 | COL |
MtCCT30 | Chr7 | Medtr7g032240.1 | 352 | 40433 | 9.53 | CMF |
MtCCT31 | Chr7 | Medtr7g083540.1 | 390 | 44311 | 6.28 | COL |
MtCCT32 | Chr7 | Medtr7g108150.1 | 372 | 42581 | 6.21 | COL |
MtCCT33 | Chr7 | Medtr7g118260.1 | 596 | 66654 | 6.54 | PRR |
MtCCT34 | Chr8 | Medtr8g024260.1 | 640 | 73001 | 6.74 | PRR |
MtCCT35 | Chr8 | Medtr8g098725.1 | 324 | 37326 | 5.34 | CMF |
MtCCT36 | Chr8 | Medtr8g104190.1 | 416 | 47041 | 5.10 | COL |
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Ma, L.; Yi, D.; Yang, J.; Liu, X.; Pang, Y. Genome-Wide Identification, Expression Analysis and Functional Study of CCT Gene Family in Medicago truncatula. Plants 2020, 9, 513. https://doi.org/10.3390/plants9040513
Ma L, Yi D, Yang J, Liu X, Pang Y. Genome-Wide Identification, Expression Analysis and Functional Study of CCT Gene Family in Medicago truncatula. Plants. 2020; 9(4):513. https://doi.org/10.3390/plants9040513
Chicago/Turabian StyleMa, Lin, Dengxia Yi, Junfeng Yang, Xiqiang Liu, and Yongzhen Pang. 2020. "Genome-Wide Identification, Expression Analysis and Functional Study of CCT Gene Family in Medicago truncatula" Plants 9, no. 4: 513. https://doi.org/10.3390/plants9040513