Heterologous Expression of a Soybean Gene RR34 Conferred Improved Drought Resistance of Transgenic Arabidopsis
Abstract
:1. Introduction
2. Results
2.1. Ectopic Expression of GmRR34 Altered the Growth Characters in Transgenic Arabidopsis Plants
2.2. Ectopic Expression of GmRR34 Improved Post-Drought Survival Rates in Transgenic Arabidopsis
2.3. GmRR34-Ectopic Expression Lines Displayed More Active Enzymatic ROS-Scavenging Activities
2.4. Stress-Related Genes with Enhanced Expression in GmRR34-Transgenic Arabidopsis
2.5. GmRR34 Ectopic Expression Lines Displayed Hypersensitivity to ABA
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Growth Conditions
4.3. Phenotypic Analyses of Ectopic Expression Lines under Normal Conditions
4.4. Survival Test
4.5. Determination of Excised-Leaf Water Loss Rate
4.6. Evaluation of H2O2 Content and ROS-Scavenging Enzymatic Activities
4.7. Expression Analysis of Transgene and Drought Response-Related Genes
4.8. ABA Sensitivity Assays
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Nghia, D.H.T.; Chuong, N.N.; Hoang, X.L.T.; Nguyen, N.C.; Tu, N.H.C.; Huy, N.V.G.; Ha, B.T.T.; Nam, T.N.H.; Thu, N.B.A.; Tran, L.-S.P.; et al. Heterologous Expression of a Soybean Gene RR34 Conferred Improved Drought Resistance of Transgenic Arabidopsis. Plants 2020, 9, 494. https://doi.org/10.3390/plants9040494
Nghia DHT, Chuong NN, Hoang XLT, Nguyen NC, Tu NHC, Huy NVG, Ha BTT, Nam TNH, Thu NBA, Tran L-SP, et al. Heterologous Expression of a Soybean Gene RR34 Conferred Improved Drought Resistance of Transgenic Arabidopsis. Plants. 2020; 9(4):494. https://doi.org/10.3390/plants9040494
Chicago/Turabian StyleNghia, Duong Hoang Trong, Nguyen Nguyen Chuong, Xuan Lan Thi Hoang, Nguyen Cao Nguyen, Nguyen Huu Cam Tu, Nguyen Van Gia Huy, Bui Thi Thanh Ha, Thai Nguyen Hoang Nam, Nguyen Binh Anh Thu, Lam-Son Phan Tran, and et al. 2020. "Heterologous Expression of a Soybean Gene RR34 Conferred Improved Drought Resistance of Transgenic Arabidopsis" Plants 9, no. 4: 494. https://doi.org/10.3390/plants9040494