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Open AccessArticle

Heterologous Expression of a Soybean Gene RR34 Conferred Improved Drought Resistance of Transgenic Arabidopsis

1
Applied Biotechnology for Crop Development Research Unit, School of Biotechnology, International University, Ho Chi Minh City 700000, Vietnam
2
Vietnam National University, Ho Chi Minh City 700000, Vietnam
3
Institute of Research and Development, Duy Tan University, 03 Quang Trung, Da Nang 550000, Vietnam
4
Stress Adaptation Research Unit, RIKEN Center for Sustainable Resource Science, 1-7-22, Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan
*
Author to whom correspondence should be addressed.
Plants 2020, 9(4), 494; https://doi.org/10.3390/plants9040494
Received: 12 March 2020 / Revised: 8 April 2020 / Accepted: 9 April 2020 / Published: 12 April 2020
Two-component systems (TCSs) have been identified as participants in mediating plant response to water deficit. Nevertheless, insights of their contribution to plant drought responses and associated regulatory mechanisms remain limited. Herein, a soybean response regulator (RR) gene RR34, which is the potential drought-responsive downstream member of a TCS, was ectopically expressed in the model plant Arabidopsis for the analysis of its biological roles in drought stress response. Results from the survival test revealed outstanding recovery ratios of 52%–53% in the examined transgenic lines compared with 28% of the wild-type plants. Additionally, remarkedly lower water loss rates in detached leaves as well as enhanced antioxidant enzyme activities of catalase and superoxide dismutase were observed in the transgenic group. Further transcriptional analysis of a subset of drought-responsive genes demonstrated higher expression in GmRR34-transgenic plants upon exposure to drought, including abscisic acid (ABA)-related genes NCED3, OST1, ABI5, and RAB18. These ectopic expression lines also displayed hypersensitivity to ABA treatment at germination and post-germination stages. Collectively, these findings indicated the ABA-associated mode of action of GmRR34 in conferring better plant performance under the adverse drought conditions. View Full-Text
Keywords: drought; GmRR34; response regulator; soybean; two-component system drought; GmRR34; response regulator; soybean; two-component system
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Nghia, D.H.T.; Chuong, N.N.; Hoang, X.L.T.; Nguyen, N.C.; Tu, N.H.C.; Huy, N.V.G.; Ha, B.T.T.; Nam, T.N.H.; Thu, N.B.A.; Tran, L.-S.P.; Thao, N.P. Heterologous Expression of a Soybean Gene RR34 Conferred Improved Drought Resistance of Transgenic Arabidopsis. Plants 2020, 9, 494.

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