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Open AccessArticle

Incidence and Molecular Identification of Apple Necrotic Mosaic Virus (ApNMV) in Southwest China

Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China
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Author to whom correspondence should be addressed.
The first two authors contributed equally to this research.
Plants 2020, 9(4), 415; https://doi.org/10.3390/plants9040415
Received: 10 February 2020 / Revised: 25 March 2020 / Accepted: 25 March 2020 / Published: 28 March 2020
(This article belongs to the Special Issue Diagnosis and Control of Plant Viral Diseases)
Apple mosaic disease has a great influence on apple production. In this study, an investigation into the incidence of apple mosaic disease in southwest China was performed, and the pathogen associated with the disease was detected. The results show that 2869 apple trees with mosaic disease were found in the Sichuan, Yunnan, and Guizhou Provinces, with an average incidence of 9.6%. Although apple mosaic virus (ApMV) is widespread in apples worldwide, the diseased samples were negative when tested for ApMV. However, a novel ilarvirus (apple necrotic mosaic virus, ApNMV) was identified in mosaic apple leaves which tested negative for ApMV. RT-PCR analysis indicated that ApNMV was detected in 322 out of 357 samples with mosaic symptoms. Phylogenetic analysis of coat protein (CP) sequences of ApNMV isolates suggested that, compared with ApMV, ApNMV was closer to prunus necrotic ringspot virus (PNRSV). The CP sequences of the isolates showed the diversity of ApNMV, which may enable the virus to adapt to the changeable environments. In addition, the pathology of mosaic disease was observed by microscope, and the result showed that the arrangement of the tissue and the shape of the cell, including the organelle, were seriously destroyed or drastically changed. View Full-Text
Keywords: ApNMV; apple mosaic disease; mosaic symptom; coat protein ApNMV; apple mosaic disease; mosaic symptom; coat protein
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MDPI and ACS Style

Shi, W.; Yao, R.; Sunwu, R.; Huang, K.; Liu, Z.; Li, X.; Yang, Y.; Wang, J. Incidence and Molecular Identification of Apple Necrotic Mosaic Virus (ApNMV) in Southwest China. Plants 2020, 9, 415.

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