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Technical Note

CRISPR/Cas9-Mediated Mutagenesis of RCO in Cardamine hirsuta

1
Department of Comparative Development and Genetics, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829 Cologne, Germany
2
Hudson River Biotechnology, Nieuwe Kanaal 7V, 6709 PA Wageningen, The Netherlands
*
Authors to whom correspondence should be addressed.
Plants 2020, 9(2), 268; https://doi.org/10.3390/plants9020268
Received: 2 February 2020 / Revised: 13 February 2020 / Accepted: 14 February 2020 / Published: 18 February 2020
(This article belongs to the Special Issue From Genes to Shape and Function: Leaf Morphogenesis at Play)
The small crucifer Cardamine hirsuta bears complex leaves divided into leaflets. This is in contrast to its relative, the reference plant Arabidopsis thaliana, which has simple leaves. Comparative studies between these species provide attractive opportunities to study the diversification of form. Here, we report on the implementation of the CRISPR/Cas9 genome editing methodology in C. hirsuta and with it the generation of novel alleles in the RCO gene, which was previously shown to play a major role in the diversification of form between the two species. Thus, genome editing can now be deployed in C. hirsuta, thereby increasing its versatility as a model system to study gene function and evolution. View Full-Text
Keywords: CRISPR/Cas9; Cardamine hirsuta; RCO; leaf development CRISPR/Cas9; Cardamine hirsuta; RCO; leaf development
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MDPI and ACS Style

Alvim Kamei, C.L.; Pieper, B.; Laurent, S.; Tsiantis, M.; Huijser, P. CRISPR/Cas9-Mediated Mutagenesis of RCO in Cardamine hirsuta. Plants 2020, 9, 268. https://doi.org/10.3390/plants9020268

AMA Style

Alvim Kamei CL, Pieper B, Laurent S, Tsiantis M, Huijser P. CRISPR/Cas9-Mediated Mutagenesis of RCO in Cardamine hirsuta. Plants. 2020; 9(2):268. https://doi.org/10.3390/plants9020268

Chicago/Turabian Style

Alvim Kamei, Claire L., Bjorn Pieper, Stefan Laurent, Miltos Tsiantis, and Peter Huijser. 2020. "CRISPR/Cas9-Mediated Mutagenesis of RCO in Cardamine hirsuta" Plants 9, no. 2: 268. https://doi.org/10.3390/plants9020268

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