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Open AccessArticle

Assessment of Genetic Relationships between Streptocarpus x hybridus V. Parents and F1 Progenies Using SRAP Markers and FT-IR Spectroscopy

1
Life Sciences Institute, Research Centre for Biotechnology in Agriculture affiliated to Romanian Academy, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5 Mănăştur St., 400372 Cluj-Napoca, Romania
2
AgroTransilvania Cluster, Dezmir, Crișeni St., FN, Dezmir 407039, Romania
3
National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donat St., 400293 Cluj-Napoca, Romania
4
Life Sciences Institute, Research Centre for Biophysics, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Faculty of Veterinary Medicine, 3-5 Mănăştur St., 400372 Cluj-Napoca, Romania
*
Authors to whom correspondence should be addressed.
Plants 2020, 9(2), 160; https://doi.org/10.3390/plants9020160
Received: 17 December 2019 / Revised: 21 January 2020 / Accepted: 25 January 2020 / Published: 28 January 2020
(This article belongs to the Section Plant Genetics, Genomics and Biotechnology)
The genetic relationship among three Streptocarpus parents and twelve F1 hybrids was assessed using sequence-related amplified polymorphism (SRAP) molecular markers and Fourier-transform infrared (FT-IR) spectroscopy. Both methods were able to discriminate F1 hybrids and parents as revealed by cluster analysis. For hybrid identification, the type III SRAP marker was the most effective due to the presence of male-specific bands in the hybrids. Different behaviors in the biochemical variability of DNA samples have been observed by FT-IR spectral analysis, which might be attributed to the inherent nature of the genomic DNA from parents and their F1 progenies. Mantel test was also carried out to compare morphological, SRAP, and FT-IR results based on genetic distances. The highest correlation coefficient was found between morphological and SRAP marker distances (R = 0.607; p ≤ 0.022). A lower correlation was observed between the morphological and FT-IR distance matrix (R = 0.231; p ≤0.008). Moreover, a positive correlation was found between the distances generated with SRAP and FT-IR analyses (R = 0.026) but was not statistically significant. These findings show that both SRAP and FT-IR techniques combined with morphological descriptions can be used effectively for nonconventional breeding programs for Streptocarpus to obtain new and valuable varieties. View Full-Text
Keywords: cape primrose; F1 hybrids; gDNA; multivariate analysis; discrimination cape primrose; F1 hybrids; gDNA; multivariate analysis; discrimination
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MDPI and ACS Style

Hârţa, M.; Borsai, O.; Muntean, C.M.; Dina, N.E.; Fǎlǎmaş, A.; Olar, L.E.; Szabo, K.; Pamfil, D.; Ştefan, R. Assessment of Genetic Relationships between Streptocarpus x hybridus V. Parents and F1 Progenies Using SRAP Markers and FT-IR Spectroscopy. Plants 2020, 9, 160. https://doi.org/10.3390/plants9020160

AMA Style

Hârţa M, Borsai O, Muntean CM, Dina NE, Fǎlǎmaş A, Olar LE, Szabo K, Pamfil D, Ştefan R. Assessment of Genetic Relationships between Streptocarpus x hybridus V. Parents and F1 Progenies Using SRAP Markers and FT-IR Spectroscopy. Plants. 2020; 9(2):160. https://doi.org/10.3390/plants9020160

Chicago/Turabian Style

Hârţa, Monica; Borsai, Orsolya; Muntean, Cristina M.; Dina, Nicoleta E.; Fǎlǎmaş, Alexandra; Olar, Loredana E.; Szabo, Katalin; Pamfil, Doru; Ştefan, Răzvan. 2020. "Assessment of Genetic Relationships between Streptocarpus x hybridus V. Parents and F1 Progenies Using SRAP Markers and FT-IR Spectroscopy" Plants 9, no. 2: 160. https://doi.org/10.3390/plants9020160

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