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Article

Dissecting the Genetic Basis of Lateral and Central Spikelet Development and Grain Traits in Intermedium-Spike Barley (Hordeum vulgare Convar. Intermedium)

1
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, OT Gatersleben, 06466 Seeland, Germany
2
Faculty of Agriculture, Cairo University, Giza 12613, Egypt
3
Institute of Agricultural and Nutritional Sciences, Faculty of Natural Sciences III, Martin Luther University Halle-Wittenberg, 06120 Halle, Germany
4
Faculty of Agriculture, Assuit University, Assuit 71526, Egypt
5
Institute of Arid Lands of Medenine, Route du Djorf km 22.5, Médénine 4100, Tunisia
*
Authors to whom correspondence should be addressed.
Plants 2020, 9(12), 1655; https://doi.org/10.3390/plants9121655
Received: 19 October 2020 / Revised: 19 November 2020 / Accepted: 23 November 2020 / Published: 26 November 2020
(This article belongs to the Section Plant Genetics, Genomics and Biotechnology)
Barley (Hordeum vulgare L.) is one of the major grain crops worldwide and considered as a model plant for temperate cereals. One of the barley row-type groups, named intermedium-barley, was used in our previous study where we reported that other genetic loci rather than vrs1 and Int-c could play a role in lateral spikelet development and even in setting grains. To continue this work, we used phenotypic and genotypic data of 254 intermedium-spike barley accessions aimed at dissecting the genetic basis of development and grain traits of lateral and central spikelet using genome wide association (GWAS) analysis. After genotypic data filtering, 8,653 single-nucleotide polymorphism (SNPs) were used for GWAS analysis. A total of 169 significant associations were identified and we focused only on the subset of associations that exceeded the p < 10−4 threshold. Thirty-three highly significant marker-trait-associations (MTAs), represented in 28 different SNPs on all seven chromosomes for the central and/or lateral spikelet traits; such as kernel length, width, area, weight, unfilled spikelet and 1000-kernel weight, were detected. Highly significant associated markers were anchored physically using barley genome sequencing to identify candidate genes to either contain the SNPs or the closest gene to the SNP position. The results showed that 12 MTAs were specific for lateral spikelet traits, nine MTAs were specific for central spikelet traits and seven MTAs for both central and lateral traits. All together, the GWAS and candidate gene results support our hypothesis that lateral spikelet development could be regulated by loci different from those regulating central spikelet development. View Full-Text
Keywords: intermedium barley; spikelet development; fertility; central spikelet; lateral spikelet; GWAS intermedium barley; spikelet development; fertility; central spikelet; lateral spikelet; GWAS
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MDPI and ACS Style

Youssef, H.M.; Allam, M.; Boussora, F.; Himmelbach, A.; Milner, S.G.; Mascher, M.; Schnurbusch, T. Dissecting the Genetic Basis of Lateral and Central Spikelet Development and Grain Traits in Intermedium-Spike Barley (Hordeum vulgare Convar. Intermedium). Plants 2020, 9, 1655. https://doi.org/10.3390/plants9121655

AMA Style

Youssef HM, Allam M, Boussora F, Himmelbach A, Milner SG, Mascher M, Schnurbusch T. Dissecting the Genetic Basis of Lateral and Central Spikelet Development and Grain Traits in Intermedium-Spike Barley (Hordeum vulgare Convar. Intermedium). Plants. 2020; 9(12):1655. https://doi.org/10.3390/plants9121655

Chicago/Turabian Style

Youssef, Helmy M., Mohamed Allam, Faiza Boussora, Axel Himmelbach, Sara G. Milner, Martin Mascher, and Thorsten Schnurbusch. 2020. "Dissecting the Genetic Basis of Lateral and Central Spikelet Development and Grain Traits in Intermedium-Spike Barley (Hordeum vulgare Convar. Intermedium)" Plants 9, no. 12: 1655. https://doi.org/10.3390/plants9121655

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