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Volume 9
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10.3390/plants9111601
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Article

DNA-Based Authentication and Metabolomics Analysis of Medicinal Plants Samples by DNA Barcoding and Ultra-High-Performance Liquid Chromatography/Triple Quadrupole Mass Spectrometry (UHPLC-MS)

by
Marta Sánchez
,
Elena González-Burgos
,
Pradeep Kumar Divakar
and
M. Pilar Gómez-Serranillos
*
Department of Pharmacology, Pharmacognosy and Botany, Faculty of Pharmacy, Universidad Complutense de Madrid (UCM), 28040 Madrid, Spain
*
Author to whom correspondence should be addressed.
Plants 2020, 9(11), 1601; https://doi.org/10.3390/plants9111601
Submission received: 28 October 2020 / Revised: 14 November 2020 / Accepted: 17 November 2020 / Published: 18 November 2020
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Abstract

There is growing interest for medicinal plants in the world drug market. Particularly, Matricaria recutita L., Valeriana officinalis L., Tilia spp., and Camellia sinensis (L.) Kuntze are some of the most consumed medicinal plants for treatment of minor health problems. Medicinal plants are seen as natural and safe; however, they can cause interactions and produce adverse reactions. Moreover, there is lack of consensus in medicinal plants regulation worldwide. DNA barcoding and UHPLC-MS technique are increasingly used to correctly identify medicinal plants and guarantee their quality and therapeutic safety. We analyzed 33 samples of valerian, linden, tea, and chamomile acquired in pharmacies, supermarkets, and herbal shops by DNA barcoding and UHPLC-MS. DNA barcoding, using matk as a barcode marker, revealed that CH1 sold as Camellia sinensis was Blepharocalyx tweediei, and sample TS2 sold as linden belong to Malvales. On the other hand, UHPLC-MS analysis revealed the presence of bioactive compounds (apigenin-7-glucoside, acetoxy valerenic acid, valerenic acid, epigallocatechin, and tiliroside). However, none of samples met minimum content of these active principles (except for valerenic acid in VF3) according to the European Medicines Agency (EMA) and Real Spanish Pharmacopeia. In conclusion, this study revealed the need to incorporate DNA barcoding and HPLC-MS techniques in quality controls of medicinal plants.
Keywords: medicinal plants; DNA barcoding; matk; HPLC-MS method medicinal plants; DNA barcoding; matk; HPLC-MS method
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MDPI and ACS Style

Sánchez, M.; González-Burgos, E.; Divakar, P.K.; Gómez-Serranillos, M.P. DNA-Based Authentication and Metabolomics Analysis of Medicinal Plants Samples by DNA Barcoding and Ultra-High-Performance Liquid Chromatography/Triple Quadrupole Mass Spectrometry (UHPLC-MS). Plants 2020, 9, 1601. https://doi.org/10.3390/plants9111601

AMA Style

Sánchez M, González-Burgos E, Divakar PK, Gómez-Serranillos MP. DNA-Based Authentication and Metabolomics Analysis of Medicinal Plants Samples by DNA Barcoding and Ultra-High-Performance Liquid Chromatography/Triple Quadrupole Mass Spectrometry (UHPLC-MS). Plants. 2020; 9(11):1601. https://doi.org/10.3390/plants9111601

Chicago/Turabian Style

Sánchez, Marta, Elena González-Burgos, Pradeep Kumar Divakar, and M. Pilar Gómez-Serranillos. 2020. "DNA-Based Authentication and Metabolomics Analysis of Medicinal Plants Samples by DNA Barcoding and Ultra-High-Performance Liquid Chromatography/Triple Quadrupole Mass Spectrometry (UHPLC-MS)" Plants 9, no. 11: 1601. https://doi.org/10.3390/plants9111601

APA Style

Sánchez, M., González-Burgos, E., Divakar, P. K., & Gómez-Serranillos, M. P. (2020). DNA-Based Authentication and Metabolomics Analysis of Medicinal Plants Samples by DNA Barcoding and Ultra-High-Performance Liquid Chromatography/Triple Quadrupole Mass Spectrometry (UHPLC-MS). Plants, 9(11), 1601. https://doi.org/10.3390/plants9111601

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