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Article

Efficacy of Antimicrobial and Larvicidal Activities of Green Synthesized Silver Nanoparticles Using Leaf Extract of Plumbago auriculata Lam

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CAS in Botany, University of Madras, Guindy Campus, Chennai 600 025, India
2
Bharath Institute of Higher Education and Research, Chennai 600 073, India
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Food Sciences Department, College of Agiculture, University of Basrah, Basrah 61004, Iraq
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ToxiVen Biotech Private Limited, Siva Nagar, Kovaipudhur 641 042, India
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Department of Chemistry, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, Tanjung Malim 35900, Malaysia
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Food, Nutrition & Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, BC V6T 1Z4, Canada
*
Authors to whom correspondence should be addressed.
Plants 2020, 9(11), 1577; https://doi.org/10.3390/plants9111577
Received: 5 October 2020 / Revised: 5 November 2020 / Accepted: 10 November 2020 / Published: 14 November 2020
This work reports the synthesis of silver nanoparticles (AgNPs) using aqueous extract of Plumbago auriculata, and evaluates their antibacterial and larvicidal activities. The synthesized silver nanoparticles were characterized by various spectroscopy techniques, such as FTIR, XRD, TEM, EDX, Zeta potential, and DLS. The synthesized AgNPs exhibited significant antibacterial activity against Gram-positive and Gram-negative bacteria, such as Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae. Furthermore, synthesized nanoparticles inhibited the fourth instars larvae of Aedes aegypti and Culex quinquefasciatus at the concentration of 45.1 and 41.1 µg/mL respectively. Results of dose-dependent studies showed that synthesized nanoparticles were also effective at low concentrations. Molecular docking studies performed with the salivary protein and odorant-binding protein of Aedes aegypti and Culex quinquefasciatus demonstrated that the naphthoquinone compound plumbagin exhibited reliable binding affinity towards the two enzymes. The findings thus reveal that the plant extract and its nanoparticles can be a better alternative to available chemicals to control mosquitos. View Full-Text
Keywords: silver nanoparticles; mosquito repellant; Plumbago auriculata; XRD; TEM; antibacterial; larvicidal activity silver nanoparticles; mosquito repellant; Plumbago auriculata; XRD; TEM; antibacterial; larvicidal activity
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MDPI and ACS Style

Govindan, L.; Anbazhagan, S.; Altemimi, A.B.; Lakshminarayanan, K.; Kuppan, S.; Pratap-Singh, A.; Kandasamy, M. Efficacy of Antimicrobial and Larvicidal Activities of Green Synthesized Silver Nanoparticles Using Leaf Extract of Plumbago auriculata Lam. Plants 2020, 9, 1577. https://doi.org/10.3390/plants9111577

AMA Style

Govindan L, Anbazhagan S, Altemimi AB, Lakshminarayanan K, Kuppan S, Pratap-Singh A, Kandasamy M. Efficacy of Antimicrobial and Larvicidal Activities of Green Synthesized Silver Nanoparticles Using Leaf Extract of Plumbago auriculata Lam. Plants. 2020; 9(11):1577. https://doi.org/10.3390/plants9111577

Chicago/Turabian Style

Govindan, Lakshmanan, Sathiyaseelan Anbazhagan, Ammar B. Altemimi, Karthik Lakshminarayanan, Sivaranjan Kuppan, Anubhav Pratap-Singh, and Murugesan Kandasamy. 2020. "Efficacy of Antimicrobial and Larvicidal Activities of Green Synthesized Silver Nanoparticles Using Leaf Extract of Plumbago auriculata Lam" Plants 9, no. 11: 1577. https://doi.org/10.3390/plants9111577

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