Molecular Detection of Potato Viruses in Bangladesh and Their Phylogenetic Analysis
Abstract
1. Introduction
2. Results
2.1. Detection of Potato Viruses
2.2. Phylogenetic Analysis
3. Discussion
4. Materials and Methods
4.1. Collection of Potato Tuber Samples
4.2. RT-PCR Detection
4.3. Cloning and Sequence Analysis
4.4. Phylogenetic Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Sample Collected Regions | No. of Collected Tuber Samples | Associated Virus Name | RT-PCR Detection | |
---|---|---|---|---|
No. of Infected Tubers | Infection Ratio (%) | |||
Munshiganj | 50 | PLRV/PVX | 7 | 14 |
PVX/PVS | 5 | 10 | ||
PLRV/PVX/PVS | 7 | 14 | ||
PLRV/PVX/PVY | 6 | 12 | ||
PLRV/PVY/PVS | 6 | 12 | ||
Jessore | 50 | PLRV/PVX | 3 | 6 |
PVX/PVS | 5 | 10 | ||
PVX/PAMV | 6 | 12 | ||
PLRV/PVX/PVS | 3 | 6 | ||
PLRV/PVY/PVS | 5 | 10 | ||
PVX/PVH/PAMV | 4 | 8 | ||
PLRV/PVX/PVH/PAMV | 5 | 10 | ||
PVX/PVS/PVH/PAMV | 3 | 6 | ||
PLRV/PVX/PVS/PVH/PAMV | 1 | 2 | ||
Bogra | 120 | PVX/PAMV | 4 | 3.33 |
PLRV/PVX/PVS | 5 | 4.17 | ||
PLRV/PVX/PVY | 4 | 3.33 | ||
PLRV/PVY/PVS | 4 | 3.33 | ||
PVX/PVH/PAMV | 11 | 9.17 | ||
PLRV/PVX/PVH/PAMV | 15 | 12.50 | ||
PVX/PVS/PVH/PAMV | 7 | 5.83 | ||
PLRV/PVX/PVS/PVH/PAMV | 4 | 3.33 | ||
PLRV/PVX/PVS/PVH/PAMV/PVM | 10 | 8.33 |
Virus Name | GenBank Accession No. | Sample Collected Regions | ||
---|---|---|---|---|
Bogra | Munshiganj | Jessore | ||
PLRV | MK445318 | + | - | - |
PLRV | MK445319 | + | + | + |
PVX | MK587458 | + | - | - |
PVX | MK587459 | + | - | - |
PVX | MF589763 | - | + | + |
PVY | MF589764 | + | + | + |
PVS | MF589762 | - | + | + |
PVS | MK587460 | + | - | - |
PVH | MK561855 | + | - | + |
PAMV | MK445320 | + | - | + |
PAMV | MK445321 | + | - | - |
PAMV | MK445322 | + | - | - |
PVM | MK445316 | + | - | - |
PVM | MK445317 | + | - | - |
Primer | Sequence (5′ to 3′) |
---|---|
PLRV-CPF | ATGAGTACGGTCGTGGTTAA |
PLRV-CPR | CTATTTGGGGTTTTGCAAAG |
PVX-CPF | TAGCCACAACACAGGCCACAG |
PVX-CPR | TTATGGTGGTGGGAGAGTGACAA |
PVY-CPF | ACGTCCAAAATGAGAATGCC |
PVY-CPR | TGGTGTTCGTGATGTGACCT |
PVS-CPF | ATGCCGCCCAAACCGGAT |
PVS-CPR | TCATTGGTTGATCGCATTAC |
PVH-CPF | ATGGATGTTGCTACTTC |
PVH-CPR | ATTGCCGCTTGTTATTC |
PAMV-CPF | ATGGTTGATTCTAAGAAAAC |
PAMV-CPR | TTAGGATGGTGGTGCCATGA |
PVM-CPF | ATGGGAGATTCAACTAAGAA |
PVM-CPR | CTTCATTTGTTATTCGACTT |
PVA-CPF | AGCCGAAACTCTTGATGCAA |
PVA-CPR | GCACTTCGGTTACACCCCCT |
PVH-TGB1F | AACTGCAGATGGATGTGCTGGTAG |
PVH-TGB1R | CGGGATCAGGCGGCGGTGAAAGTG |
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Rashid, M.-O.; Wang, Y.; Han, C.-G. Molecular Detection of Potato Viruses in Bangladesh and Their Phylogenetic Analysis. Plants 2020, 9, 1413. https://doi.org/10.3390/plants9111413
Rashid M-O, Wang Y, Han C-G. Molecular Detection of Potato Viruses in Bangladesh and Their Phylogenetic Analysis. Plants. 2020; 9(11):1413. https://doi.org/10.3390/plants9111413
Chicago/Turabian StyleRashid, Mamun-Or, Ying Wang, and Cheng-Gui Han. 2020. "Molecular Detection of Potato Viruses in Bangladesh and Their Phylogenetic Analysis" Plants 9, no. 11: 1413. https://doi.org/10.3390/plants9111413
APA StyleRashid, M.-O., Wang, Y., & Han, C.-G. (2020). Molecular Detection of Potato Viruses in Bangladesh and Their Phylogenetic Analysis. Plants, 9(11), 1413. https://doi.org/10.3390/plants9111413