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Open AccessArticle

Comparative Physiological and Proteomic Analysis Reveals Different Involvement of Proteins during Artificial Aging of Siberian Wildrye Seeds

1
College of Animal science and Technology, Sichuan Agricultural University, Chengdu 611130, China
2
Sichuan Academy of Grassland Science, Chengdu 611731, China
3
Key Laboratory of Superior Forage Germplasm in the Qinghai-Tibetan Plateau / Qinghai Academy of Animal Science and Veterinary Medicine, Xining 810016, Qinghai, China
4
College of Environmental Sciences, Sichuan Agricultural University, Chengdu 611130, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Plants 2020, 9(10), 1370; https://doi.org/10.3390/plants9101370
Received: 27 September 2020 / Revised: 13 October 2020 / Accepted: 13 October 2020 / Published: 15 October 2020
(This article belongs to the Special Issue Responses of Plants to Environmental Stresses)
Seed aging has an important effect on the germplasm preservation and industrialized production of Siberian wildrye (Elymus sibiricus) in the Qinghai-Tibet Plateau. However, so far its underlying molecular mechanisms still largely remain unknown. To shed light on this topic, one-year stored seeds of E. sibiricus were exposed to artificial aging treatments (AAT), followed by seed vigor characteristics and physiological status monitoring. Then global proteomics analysis was undertaken by the tandem mass tags (TMT) technique, and the proteins were quantified with liquid chromatography-tandem mass spectrometry on three aging time points (0 h, 36 h and 72 h). Finally, we verified the expression of related proteins by parallel reaction monitoring (PRM). Our results demonstrated that the seed vigor decreased remarkably in response to artificial aging, but the relative ion-leakage and malondialdehyde content, superoxide anion and hydrogen peroxide showed the opposite situation. Proteomic results showed that a total of 4169 proteins were identified and quantified. Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that a series of key pathways including carbohydrate metabolism, lipid metabolism, and antioxidant activity were severely damaged by aging treatments. Numerous key proteins such as glyceraldehyde triphosphate glyceraldehyde dehydrogenase, succinate dehydrogenase, lipoxygenase, peroxidase, glutathione-s-transferase and late embryogenesis abundant proteins were significantly down-regulated. However, the up-regulation of the heat shock protein family has made a positive contribution to oxidative stress resistance in seeds. This study provides a useful catalog of the E. sibiricus proteomes with insights into the future genetic improvement of seed storability. View Full-Text
Keywords: Elymus sibiricus, seed aging; isobaric tandem mass tag labeling; reactive oxygen species; parallel reaction monitoring Elymus sibiricus, seed aging; isobaric tandem mass tag labeling; reactive oxygen species; parallel reaction monitoring
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Lei, X.; Liu, W.; Zhao, J.; You, M.; Xiong, C.; Xiong, Y.; Xiong, Y.; Yu, Q.; Bai, S.; Ma, X. Comparative Physiological and Proteomic Analysis Reveals Different Involvement of Proteins during Artificial Aging of Siberian Wildrye Seeds. Plants 2020, 9, 1370.

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