Next Article in Journal
Strigolactone Signaling Genes Showing Differential Expression Patterns in Arabidopsis max Mutants
Previous Article in Journal
Antimicrobial Activity, Antioxidant Potential, Cytotoxicity and Phytochemical Profiling of Four Plants Locally Used against Skin Diseases
Open AccessArticle

H2O2 Induces Association of RCA with the Thylakoid Membrane to Enhance Resistance of Oryza meyeriana to Xanthomonas oryzae pv. oryzae

1
College of Plant Protection, Shenyang Agricultural University, Shenyang 210095, China
2
State Key Laboratory for Quality and Safety of Agro-products, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China
3
College of Plant Protection, Yunnan Agricultural University, Kunming 650000, China
4
Institute of Plant Virology, Ningbo University, Ningbo 315211, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Plants 2019, 8(9), 351; https://doi.org/10.3390/plants8090351
Received: 13 August 2019 / Revised: 4 September 2019 / Accepted: 10 September 2019 / Published: 16 September 2019
(This article belongs to the Section Plant Physiology and Metabolism)
Oryza meyeriana is a wild species of rice with high resistance to Xanthomonas oryzae pv. oryzae (Xoo), but the detailed resistance mechanism is unclear. Ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) activase (RCA) is an important enzyme that regulates photosynthesis by activating Rubisco. We have previously reported that Xoo infection induced the relocation of RCA from the chloroplast stroma to the thylakoid membrane in O. meyeriana, but the underlying regulating mechanism and physiological significance of this association remains unknown. In this study, “H2O2 burst” with rapid and large increase in the amount of H2O2 was found to be induced by Xoo invasion in the leaves of O. meyeriana. 3, 3-diaminobenzidine (DAB) and oxidative 2, 7-Dichlorodi-hydrofluorescein diacetate (H2DCFDA) staining experiments both showed that H2O2 was generated in the chloroplast of O. meyeriana, and that this H2O2 generation as well as Xoo resistance of the wild rice were dramatically dependent on light. H2O2, methyl viologen with light, and the xanthine-xanthine oxidase system all induced RCA to associate with the thylakoid membrane in vitro, which showed that H2O2 could induce the relocation of RCA. In vitro experiments also showed that H2O2 induced changes in both the RCA and thylakoid membrane that were required for them to associate and that this association only occurred in O. meyeriana and not in the susceptible cultivated rice. These results suggest that the association of RCA with the thylakoid membrane helps to protect the thylakoid membrane against oxidative damage from H2O2. Therefore, in addition to its universal function of activating Rubisco, RCA appears to play a novel role in the resistance of O. meyeriana to Xoo. View Full-Text
Keywords: H2O2; Rubisco activase; thylakoid membrane; chloroplast stoma; Xanthomonas oryzae pv. oryzae; Oryza meyeriana H2O2; Rubisco activase; thylakoid membrane; chloroplast stoma; Xanthomonas oryzae pv. oryzae; Oryza meyeriana
Show Figures

Figure 1

MDPI and ACS Style

Mei, Q.; Yang, Y.; Ye, S.; Liang, W.; Wang, X.; Zhou, J.; Yu, C.; Yan, C.; Chen, J. H2O2 Induces Association of RCA with the Thylakoid Membrane to Enhance Resistance of Oryza meyeriana to Xanthomonas oryzae pv. oryzae. Plants 2019, 8, 351.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop