H2O2 Induces Association of RCA with the Thylakoid Membrane to Enhance Resistance of Oryza meyeriana to Xanthomonas oryzae pv. oryzae
Abstract
:1. Introduction
2. Results
2.1. Resistance Analysis
2.2. Effect of Xoo Inoculation on RCA Distribution
2.3. Effect of Xoo Inoculation on H2O2 Generation
2.4. Subcellular Localization of H2O2
2.5. Light-Dependent H2O2 Generation and Resistance in Wild Rice
2.6. Effect of H2O2 on RCA Redistribution in Vitro
2.7. In Vitro Investigation of the Association between RCA and Thylakoid Membrane
3. Discussion
3.1. High Resistance of O. meyeriana to Xoo
3.2. H2O2 Generation in Chloroplasts of O. meyeriana Infected by Xoo
3.3. Chloroplast H2O2 Induced the Association of RCA with Thylakoid Membrane Following Infection of O. meyeriana with Xoo
3.4. Potential Novel Role of RCA in the Xoo Resistance of O. meyeriana
4. Materials and Methods
4.1. Plant Materials
4.2. Pathogen Inoculation and Resistance Identification
4.3. Measurements of Chlorophyll Fluorescence
4.4. Isolation and Sub-Fractionation of Chloroplasts
4.5. SDS-PAGE and Immunological Analyses
4.6. Determination of H2O2 in Leaf extracts
4.7. Subcellular Localization of H2O2
4.8. Salt Washing and Thermolysin Treatment of Isolated Thylakoids
4.9. Oxidative Treatment of Ruptured Chloroplasts
4.10. Shading Treatment of Wild Rice Leaves
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Mei, Q.; Yang, Y.; Ye, S.; Liang, W.; Wang, X.; Zhou, J.; Yu, C.; Yan, C.; Chen, J. H2O2 Induces Association of RCA with the Thylakoid Membrane to Enhance Resistance of Oryza meyeriana to Xanthomonas oryzae pv. oryzae. Plants 2019, 8, 351. https://doi.org/10.3390/plants8090351
Mei Q, Yang Y, Ye S, Liang W, Wang X, Zhou J, Yu C, Yan C, Chen J. H2O2 Induces Association of RCA with the Thylakoid Membrane to Enhance Resistance of Oryza meyeriana to Xanthomonas oryzae pv. oryzae. Plants. 2019; 8(9):351. https://doi.org/10.3390/plants8090351
Chicago/Turabian StyleMei, Qiong, Yong Yang, Shenhai Ye, Weifang Liang, Xuming Wang, Jie Zhou, Chulang Yu, Chengqi Yan, and Jianping Chen. 2019. "H2O2 Induces Association of RCA with the Thylakoid Membrane to Enhance Resistance of Oryza meyeriana to Xanthomonas oryzae pv. oryzae" Plants 8, no. 9: 351. https://doi.org/10.3390/plants8090351