Functional Characterization of Physcomitrella patens Glycerol-3-Phosphate Acyltransferase 9 and an Increase in Seed Oil Content in Arabidopsis by Its Ectopic Expression
Abstract
:1. Introduction
2. Results
2.1. Isolation of GPAT9 From P. patens
2.2. Expression of PpGPAT9 in the Protonema and Gametophores
2.3. Subcellular Localization of PpGPAT9
2.4. Generation of Arabidopsis Transgenic Plants Ectopically Expressing PpGPAT9
2.5. Morphological and Microscopic Analyses of Transgenic Arabidopsis Seeds Ectopically Expressing PpGPAT9
2.6. Fatty Acid Analysis of Arabidopsis Seeds and Leaves Ectopically Expressing PpGPAT9
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Phylogenetic Tree Construction
4.3. RNA Isolation and Gene Expression Analysis
4.4. Subcellular Localization Using Fluorescent Reporter Proteins
4.5. Ectopic Expression and Transformation
4.6. Protein Extraction and Western Blotting
4.7. Microscopic Analysis of Seeds
4.8. Fatty Acid Analysis
4.9. Transient Expression in N. benthamiana Leaves
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Yang, S.U.; Kim, J.; Kim, H.; Suh, M.C. Functional Characterization of Physcomitrella patens Glycerol-3-Phosphate Acyltransferase 9 and an Increase in Seed Oil Content in Arabidopsis by Its Ectopic Expression. Plants 2019, 8, 284. https://doi.org/10.3390/plants8080284
Yang SU, Kim J, Kim H, Suh MC. Functional Characterization of Physcomitrella patens Glycerol-3-Phosphate Acyltransferase 9 and an Increase in Seed Oil Content in Arabidopsis by Its Ectopic Expression. Plants. 2019; 8(8):284. https://doi.org/10.3390/plants8080284
Chicago/Turabian StyleYang, Sun Ui, Juyoung Kim, Hyojin Kim, and Mi Chung Suh. 2019. "Functional Characterization of Physcomitrella patens Glycerol-3-Phosphate Acyltransferase 9 and an Increase in Seed Oil Content in Arabidopsis by Its Ectopic Expression" Plants 8, no. 8: 284. https://doi.org/10.3390/plants8080284