Molecular Insights into FaEG1, a Strawberry Endoglucanase Enzyme Expressed during Strawberry Fruit Ripening
Abstract
:1. Introduction
2. Methods
2.1. Plant Material
2.2. RNA Isolation, Reverse Transcription and Reverse-Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) Analysis
2.3. Scanning Electron Microscopy (SEM)
2.4. Sequence Comparison and Phylogenic Analysis
2.5. FaEG1 Structural Model Obtained by Comparative Modeling
2.6. Protein–Ligand Interaction Determinations
3. Results and Discussion
3.1. Sequence and Phylogenetic Analyses
3.2. Transcriptional Profile Analysis of the FaEG1 Gene
3.3. SEM Studies of the Surface Morphology of Strawberries
3.4. Obtaining the FaEG1 Protein Model
3.5. 3D Structure of the FaEG1 Protein Model
3.6. Protein–Ligand Interactions
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Complex | Binding Energy (Autodock Vina Score) | |
---|---|---|
FcEG1 | cellodextrin 8-mer | −7.5 a ± 0.4 |
XXXGXXXG | −6.9 ab ± 0.5 | |
XXFGXXFG | −5.9 b ± 0.5 |
Complex | ΔHvdWMM (kcal mol−1) | ΔHelecMM (kcal mol−1) | ΔGsol–pol (kcal mol−1) | ΔGsol–npol (kcal mol−1) | ΔGbind (kcal mol−1) | |
---|---|---|---|---|---|---|
FcEG1 | Cellulose | −67.21 | −71.11 | 67.30 | −52.30 | −123.32 ± 9 a |
XXXGXXXG | −55.21 | −58.14 | 63.10 | −25.06 | −75.31 ± 5 ab | |
XXFGXXFG | −52.85 | −26.51 | 46.68 | −30.97 | −63.65 ± 8 b |
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Jara, K.; Castro, R.I.; Ramos, P.; Parra-Palma, C.; Valenzuela-Riffo, F.; Morales-Quintana, L. Molecular Insights into FaEG1, a Strawberry Endoglucanase Enzyme Expressed during Strawberry Fruit Ripening. Plants 2019, 8, 140. https://doi.org/10.3390/plants8060140
Jara K, Castro RI, Ramos P, Parra-Palma C, Valenzuela-Riffo F, Morales-Quintana L. Molecular Insights into FaEG1, a Strawberry Endoglucanase Enzyme Expressed during Strawberry Fruit Ripening. Plants. 2019; 8(6):140. https://doi.org/10.3390/plants8060140
Chicago/Turabian StyleJara, Karla, Ricardo I. Castro, Patricio Ramos, Carolina Parra-Palma, Felipe Valenzuela-Riffo, and Luis Morales-Quintana. 2019. "Molecular Insights into FaEG1, a Strawberry Endoglucanase Enzyme Expressed during Strawberry Fruit Ripening" Plants 8, no. 6: 140. https://doi.org/10.3390/plants8060140