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Molecular Insights into FaEG1, a Strawberry Endoglucanase Enzyme Expressed during Strawberry Fruit Ripening

1
Instituto de Ciencias Biológicas, Universidad de Talca, Talca 3465548, Chile
2
Multidisciplinary Agroindustry Research Laboratory, Instituto de Químicas Aplicadas, Universidad Autónoma de Chile, Talca 3460000, Chile
3
Núcleo Científico Multidiciplinario-DI, Universidad de Talca, Talca 3465548, Chile
4
Multidisciplinary Agroindustry Research Laboratory, Instituto de Ciencias Biomédicas, Facultado de Ingeniería, Universidad Autónoma de Chile, Talca 3460000, Chile
*
Authors to whom correspondence should be addressed.
Plants 2019, 8(6), 140; https://doi.org/10.3390/plants8060140
Received: 23 April 2019 / Revised: 18 May 2019 / Accepted: 25 May 2019 / Published: 28 May 2019
(This article belongs to the Special Issue Plant Cell Wall Biology)
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Abstract

The endo-β-1,4-glucanases (EGs) that belong to the glycosyl hydrolase family 9 (GH9) have roles in cell wall synthesis, remodeling and degradation. Previous studies have suggested that EGs may play a key role in the ripening of different fruits including strawberries. In this study, we used reverse-transcription quantitative polymerase chain reaction (RT-qPCR) assays to determine the transcript accumulation of an endo-β-1,4-glucanase (FaEG1) during fruit development in two different strawberry ‘Camarosa’ and ‘Monterey’ with contrasting softening ratios. Phylogenetic analyses suggest that FaEG1 belongs to the α group of the GH9 family with other proteins previously described with roles in elongation, abscission and ripening. Comparative modeling was used to obtain the FaEG1 structure. The model displays a α-barrel–type structure that is typical of the GH9 enzyme family, and comprises 12 α-helices, 2 310 helices and 6 β-sheets. The catalytic residues were oriented to the solvent in the middle of an open groove. Protein–ligand interactions were explored with cellulose and two xyloglucans as ligands; the results suggest that the FaEG1-cellulose and FaEG1-XXXGXXXG (the most abundant xyloglucan in strawberries) complexes were more stable complexes than XXFGXXFG. The cell wall degradation was observed by scanning electron microscopy (SEM). The data are congruent with the probable role of the FaEG1 protein in the dissembly of the cellulose-hemicellulose fraction during the ripening of strawberry fruit. View Full-Text
Keywords: endoglucanase; cell wall disassembly; molecular modeling; molecular dynamic simulations; strawberry endoglucanase; cell wall disassembly; molecular modeling; molecular dynamic simulations; strawberry
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Jara, K.; Castro, R.I.; Ramos, P.; Parra-Palma, C.; Valenzuela-Riffo, F.; Morales-Quintana, L. Molecular Insights into FaEG1, a Strawberry Endoglucanase Enzyme Expressed during Strawberry Fruit Ripening. Plants 2019, 8, 140.

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