Next Article in Journal
Organic Certification is Not Enough: The Case of the Methoxydecane Frankincense
Previous Article in Journal
Light-Induced Vitamin C Accumulation in Tomato Fruits is Independent of Carbohydrate Availability
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessArticle

Sequencing and Structural Analysis of the Complete Chloroplast Genome of the Medicinal Plant Lycium chinense Mill

DNA Barcoding Laboratory for TCM Authentication, Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
Pharmaceutical School, YouJiang Medical University for Nationalities, Baise 533000, China
Authors to whom correspondence should be addressed.
These authors have contributed equally to this work.
Plants 2019, 8(4), 87;
Received: 21 February 2019 / Revised: 29 March 2019 / Accepted: 31 March 2019 / Published: 3 April 2019
(This article belongs to the Special Issue Plant Bioinformatics)
PDF [3955 KB, uploaded 17 April 2019]
  |     |  


Lycium chinense Mill, an important Chinese herbal medicine, is widely used as a dietary supplement and food. Here the chloroplast (CP) genome of L. chinense was sequenced and analyzed, revealing a size of 155,756 bp and with a 37.8% GC content. The L. chinense CP genome comprises a large single copy region (LSC) of 86,595 bp and a small single copy region (SSC) of 18,209 bp, and two inverted repeat regions (IRa and IRb) of 25,476 bp separated by the single copy regions. The genome encodes 114 genes, 16 of which are duplicated. Most of the 85 protein-coding genes (CDS) had standard ATG start codons, while 3 genes including rps12, psbL and ndhD had abnormal start codons (ACT and ACG). In addition, a strong A/T bias was found in the majority of simple sequence repeats (SSRs) detected in the CP genome. Analysis of the phylogenetic relationships among 16 species revealed that L. chinense is a sister taxon to Lycium barbarum. Overall, the complete sequence and annotation of the L. chinense CP genome provides valuable genetic information to facilitate precise understanding of the taxonomy, species and phylogenetic evolution of the Solanaceae family. View Full-Text
Keywords: Lycium chinense Mill; chloroplast genome; phylogenetic analysis Lycium chinense Mill; chloroplast genome; phylogenetic analysis

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Yang, Z.; Huang, Y.; An, W.; Zheng, X.; Huang, S.; Liang, L. Sequencing and Structural Analysis of the Complete Chloroplast Genome of the Medicinal Plant Lycium chinense Mill. Plants 2019, 8, 87.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Plants EISSN 2223-7747 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top