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Open AccessArticle

The Plant NF-Y DNA Matrix In Vitro and In Vivo

Dipartimento di Bioscienze, Università degli Studi di Milano, Via Celoria 26, 20133 Milano, Italy
Author to whom correspondence should be addressed.
Plants 2019, 8(10), 406;
Received: 1 August 2019 / Revised: 24 September 2019 / Accepted: 8 October 2019 / Published: 10 October 2019
(This article belongs to the Section Plant Genetics and Genomics)
Nuclear Factor Y (NF-Y) is an evolutionarily conserved trimer formed by a Histone-Fold Domain (HFD) heterodimeric module shared by core histones, and the sequence-specific NF-YA subunit. In plants, the genes encoding each of the three subunits have expanded in number, giving rise to hundreds of potential trimers. While in mammals NF-Y binds a well-characterized motif, with a defined matrix centered on the CCAAT box, the specificity of the plant trimers has yet to be determined. Here we report that Arabidopsis thaliana NF-Y trimeric complexes, containing two different NF-YA subunits, bind DNA in vitro with similar affinities. We assayed precisely sequence-specificity by saturation mutagenesis, and analyzed genomic DNA sites bound in vivo by selected HFDs. The plant NF-Y CCAAT matrix is different in nucleotides flanking CCAAT with respect to the mammalian matrix, in vitro and in vivo. Our data point to flexible DNA-binding rules by plant NF-Ys, serving the scope of adapting to a diverse audience of genomic motifs. View Full-Text
Keywords: Transcription Factor; plant NF-Y; TFBS; DNA matrix; LEC1 Transcription Factor; plant NF-Y; TFBS; DNA matrix; LEC1
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Gnesutta, N.; Chiara, M.; Bernardini, A.; Balestra, M.; Horner, D.S.; Mantovani, R. The Plant NF-Y DNA Matrix In Vitro and In Vivo. Plants 2019, 8, 406.

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