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Open AccessArticle

The Plant NF-Y DNA Matrix In Vitro and In Vivo

Dipartimento di Bioscienze, Università degli Studi di Milano, Via Celoria 26, 20133 Milano, Italy
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Plants 2019, 8(10), 406; https://doi.org/10.3390/plants8100406
Received: 1 August 2019 / Revised: 24 September 2019 / Accepted: 8 October 2019 / Published: 10 October 2019
(This article belongs to the Section Plant Genetics and Genomics)
Nuclear Factor Y (NF-Y) is an evolutionarily conserved trimer formed by a Histone-Fold Domain (HFD) heterodimeric module shared by core histones, and the sequence-specific NF-YA subunit. In plants, the genes encoding each of the three subunits have expanded in number, giving rise to hundreds of potential trimers. While in mammals NF-Y binds a well-characterized motif, with a defined matrix centered on the CCAAT box, the specificity of the plant trimers has yet to be determined. Here we report that Arabidopsis thaliana NF-Y trimeric complexes, containing two different NF-YA subunits, bind DNA in vitro with similar affinities. We assayed precisely sequence-specificity by saturation mutagenesis, and analyzed genomic DNA sites bound in vivo by selected HFDs. The plant NF-Y CCAAT matrix is different in nucleotides flanking CCAAT with respect to the mammalian matrix, in vitro and in vivo. Our data point to flexible DNA-binding rules by plant NF-Ys, serving the scope of adapting to a diverse audience of genomic motifs. View Full-Text
Keywords: Transcription Factor; plant NF-Y; TFBS; DNA matrix; LEC1 Transcription Factor; plant NF-Y; TFBS; DNA matrix; LEC1
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Gnesutta, N.; Chiara, M.; Bernardini, A.; Balestra, M.; Horner, D.S.; Mantovani, R. The Plant NF-Y DNA Matrix In Vitro and In Vivo. Plants 2019, 8, 406.

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