Nitric Oxide Enhances Cytotoxicity of Lead by Modulating the Generation of Reactive Oxygen Species and Is Involved in the Regulation of Pb2+ and Ca2+ Fluxes in Tobacco BY-2 Cells
Abstract
:1. Introduction
2. Results
2.1. Pb Induced PCD in Tobacco BY-2 Cells
2.2. Pb Triggered ROS and NO Bursts in Tobacco BY-2 Cells
2.3. NO Contributed to Pb-Induced ROS Production in Tobacco BY-2 Cells
2.4. NO Increased Pb2+ Influx in Tobacco BY-2 Cells
2.5. NO Promoted Pb Uptake to Aggravate Pb Toxicity
2.6. NO Enhanced Pb-Induced Calcium Homeostasis Disorder
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Hoechst and PI Double Staining
4.3. Detection of NO and ROS Production
4.4. Determination of Pb Content
4.5. Measurement of Pb2+ and Ca2+ Fluxes
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Wu, J.; Zhang, Y.; Hao, R.; Cao, Y.; Shan, X.; Jing, Y. Nitric Oxide Enhances Cytotoxicity of Lead by Modulating the Generation of Reactive Oxygen Species and Is Involved in the Regulation of Pb2+ and Ca2+ Fluxes in Tobacco BY-2 Cells. Plants 2019, 8, 403. https://doi.org/10.3390/plants8100403
Wu J, Zhang Y, Hao R, Cao Y, Shan X, Jing Y. Nitric Oxide Enhances Cytotoxicity of Lead by Modulating the Generation of Reactive Oxygen Species and Is Involved in the Regulation of Pb2+ and Ca2+ Fluxes in Tobacco BY-2 Cells. Plants. 2019; 8(10):403. https://doi.org/10.3390/plants8100403
Chicago/Turabian StyleWu, Jiaye, Yue Zhang, Ruizhi Hao, Yuan Cao, Xiaoyi Shan, and Yanping Jing. 2019. "Nitric Oxide Enhances Cytotoxicity of Lead by Modulating the Generation of Reactive Oxygen Species and Is Involved in the Regulation of Pb2+ and Ca2+ Fluxes in Tobacco BY-2 Cells" Plants 8, no. 10: 403. https://doi.org/10.3390/plants8100403