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Open AccessArticle

Alleviation of Phytophthora infestans Mediated Necrotic Stress in the Transgenic Potato (Solanum tuberosum L.) with Enhanced Ascorbic acid Accumulation

1
Department of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University, Seoul 05029, Korea
2
Department of Biotechnology, Bharathiar University, Coimbatore 641046, Tamil Nadu, India
3
Laboratory of Plant Molecular Biology, Department of Biotechnology, Dr. Harisingh Gour Central University, Sagar 470003, Madhya Pradesh, India
4
Department of Botany, Bharathiar University, Coimbatore 641046, Tamil Nadu, India
*
Author to whom correspondence should be addressed.
Plants 2019, 8(10), 365; https://doi.org/10.3390/plants8100365
Received: 9 June 2019 / Revised: 2 September 2019 / Accepted: 4 September 2019 / Published: 23 September 2019
Potato is the most widely cultivated non-cereal crop in the world, and like any other crop, it is susceptible to yield losses because of various factors, including pathogen attacks. Among the various diseases of potato, late blight caused by the oomycete Phytophthora infestans is considered as the most devastating disease worldwide. In this study, transgenic potato plants overexpressing the D-galacturonic acid reductase (GalUR) gene with an enhanced level of cellular L-ascorbate (L-AsA) were challenged with Phytophthora infestans to determine the level of stress tolerance induced in those plants. With the onset of pathogen infection, necrotic lesions progressively expanded and became necrotic in the control plants. The transgenic potato lines with enhanced ascorbic acid showed reduced necrotic lesions. Hydrogen peroxide (H2O2) and malondialdehyde (MDA) levels were relatively lower in transgenic plants compared to the untransformed control (UT) plants. The mRNA expressions of pathogenesis-related (PR) genes, such as pathogenesis related 1 (PR1) and phenylalanine ammonia-lyase (PAL) were slightly higher in GalUR overexpressing transgenic lines as compared to the untransformed control plants. Pathogen infection also altered the mRNA expression of genes associated with gibberellic acid (GA) and abscisic acid (ABA) biosynthesis. Furthermore, the increase in various antioxidant enzymes was also observed in the gene expression analysis with the transgenic plants. The complete loss of the pathogen growth and disease occurrence was not observed in our study; however, the findings indicated that an increase in the level of cellular L-ascorbate in the transgenic potato leads to enhanced cellular antioxidants, PR genes and plant defense hormones, such as GA and ABA resulting in the reduction of the disease symptoms caused by the Phytophthora infestans. View Full-Text
Keywords: ascorbic acid; Phytophthora infestans; reactive oxygen species; Solanum tuberosum; transgenic plants ascorbic acid; Phytophthora infestans; reactive oxygen species; Solanum tuberosum; transgenic plants
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Chung, I.-M.; Venkidasamy, B.; Upadhyaya, C.P.; Packiaraj, G.; Rajakumar, G.; Thiruvengadam, M. Alleviation of Phytophthora infestans Mediated Necrotic Stress in the Transgenic Potato (Solanum tuberosum L.) with Enhanced Ascorbic acid Accumulation. Plants 2019, 8, 365.

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